Yese transcriptome sequencing - Illumina
Features
● Dual raibhurari yekuteedzera iyo yakazara transcriptome: rRNA depletion inoteverwa nePE150 raibhurari kugadzirira uye saizi sarudzo inoteverwa neSE50 raibhurari kugadzirira.
● Zadzisa bioinformatics ongororo yemRNA, lncRNA, circRNA uye miRNA mune akasiyana bioinformatics mishumo.
● Kuongororwa kwakabatana kwese RNA kutaura mushumo yakasanganiswa, kusanganisira ceRNA network analysis.
Basa Rinobatsira
●Kuongorora kwakadzama kwema network network: ceRNA network yekuongorora inogoneswa nekutevedzana kwakabatana kwemRNA, lncRNA, circRNA uye miRNA uye nekupedza bioinformatic workflow.
●Comprehensive Annotation: Isu tinoshandisa akawanda dhatabhesi kuti tinyatso tsanangura iyo Differentially Inoratidzwa Genes (DEGs) uye kuita inoenderana ongororo yekuvandudza, ichipa ruzivo pane maserura uye mamorekuru maitiro ari pasi pemhinduro yezvinyorwa.
●Hunyanzvi Hwakawanda: ine rekodhi rekodhi yekuvhara zvakabudirira pamusoro pe2000 yakazara mapurojekiti ezvinyorwa munzvimbo dzakasiyana siyana dzekutsvagisa, timu yedu inounza hupfumi hweruzivo kupurojekiti yega yega.
●Rigorous Quality Control: Isu tinoshandisa epakati kudzora mapoinzi pamatanho ese, kubva kumuenzaniso uye kugadzirira raibhurari kune kutevedzana uye bioinformatics.Uku kutarisisa kwakadzama kunovimbisa kuburitswa kwezvinogara zvemhando yepamusoro mhedzisiro.
● Chirevo Chakakwana: Isu tinoshandisa akawanda dhatabhesi kuti tinyatso tsanangura iyo Differentially Inoratidzwa Genes (DEGs) uye kuita inoenderana ongororo yekuvandudza, ichipa ruzivo pane maserura uye mamorekuru maitiro ari pasi pemhinduro yezvinyorwa.
●Post-Sales Support: Kuzvipira kwedu kunowedzera kupfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi nguva.Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.
Sample Zvinodiwa uye Delivery
| Library | Sequencing strategy | Data inokurudzirwa | Quality Control |
| rRNA yakapera | Illumina PE150 | 16 Gb | Q30≥85% |
| Saizi yakasarudzwa | Illumina SE50 | 10-20M inoverengwa |
Sample Zvinodiwa:
Nucleotides:
| Conc.(ng/μl) | Mari (μg) | Kuchena | Kuperera |
| ≥ 100 | ≥ 1 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel. | Zvirimwa: RIN≥6.5 Mhuka: RIN≥7.0 5.0≥28S/18S≥1.0; kukwirira kwakadzika kana kusina kwekutanga |
Yakakurudzirwa Sample Delivery
Container:
2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)
Sample labeling: Boka+ replicate eg A1, A2, A3;B1, B2, B3...
Shipment:
1.Dry-ice: Samples dzinoda kuiswa mumabhegi uye kuvigwa muoma-ice.
2.RNAstable tubes: RNA samples inogona kuomeswa muRNA stabilization tube (eg RNAstable®) uye inotumirwa mukupisa kwekamuri.
Kuyerera Kwebasa Rebasa
Kuedza kugadzira
Muenzaniso wekutumira
RNA kubviswa
Kuvakwa kweraibhurari
Sequencing
Data analysis
Mushure mekutengesa masevhisi
Bioinformatics
RNA kutaura muchidimbu
Differentially Akaratidza majini
ceRNA kuongorora
Yakasiyana inoratidzwa miRNAs uye ine hukama RNAs
Ongorora kufambira mberi kwekutsvagisa kunofambiswa neBMKGene 'yose transcriptome sequencing masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.
Dai, Y. et al.(2022) 'Comprehensive expression profiles of mRNAs, lncRNAs uye miRNAs muKashin-Beck chirwere chakaonekwa neRNA-sequencing', Molecular Omics, 18 (2), pp. 154-166.doi: 10.1039/D1MO00370D.
Liu, N. nan et al.(2022) 'Full urefu transcriptomes wongororo yekutonhora-kuramba kweApis cerana muChangbai Mountain panguva yechando.', Gene, 830, pp. 146503–146503.doi: 10.1016/J.GENE.2022.146503.
Wang, XJ nevamwe.(2022) 'Multi-Omics Kubatanidzwa-Yakavakirwa Kutungamira Kwekukwikwidza Endogenous RNA Regulation Networks muDiki Cell Lung Cancer: Molecular Characteristics uye Drug Candidates', Frontiers muOncology, 12, p.904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xu, P. et al.(2022) 'Kuongorora kwakabatanidzwa kwelncRNA/circRNA-miRNA-mRNA kutaura kweprofiles kunoratidza ruzivo rutsva mune zvingangoita nzira mukupindura midzi-knot nematodes munzungu', BMC Genomics, 23 (1), mapeji 1-12.doi: 10.1186/S12864-022-08470-3/FIGURES/7.
Yan, Z. et al.(2022) 'Whole-transcriptome RNA sequencing inosimbisa nzira dzemamorekuru dzine chekuita nekuchengetedzwa kwemhando yepostharvest mu broccoli nered LED irradiation', Postharvest Biology and Technology, 188, p.111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.
