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Yese genome bisulfite kutevedzana

DNA methylation panzvimbo yechishanu mu cytosine (5-mC) ine simba guru pakutaura kwemajini uye kushandiswa kwemasero.Abnormal methylation maitiro akave akabatanidzwa neanoverengeka mamiriro uye zvirwere, senge kenza.WGBS yave chiyero chegoridhe chekudzidza genome-wide methylation pane imwechete base resolution.

Platform: Illumina NovaSeq Platform


Service Details

Bioinformatics

Demo Results

Basa Rinobatsira

● Complete platform: ipai imwe-stop yakanakisa sevhisi kubva pakugadziriswa kwemuenzaniso, kuvaka raibhurari, kutevedzana kune bioinformatics kuongororwa.

● Kururamisa kwepamusoro: Kukura methylation kutendeuka kwekugadzirisa teknolojia inogona kunyatsoongorora mamiriro emethylation yeC imwe nheyo.

● Wide coverage: kuonekwa kwe methylation nzvimbo pa genome-wide level.

● Kudzokorora kwakanaka: kushandiswa kwakagadzikana, kwakakodzera kuongororwa kwekuenzanisa pakati pemasampuli akawanda.

 

Sample Specifications

 

Platform

 

 

Read Length

 

 

Sequencing Strategy

 

Illumina

PE150

30X, matatu biological replicates, ane high quality reference genome

Mienzaniso Zvinodiwa

 

Muenzaniso Type

 

 

Mari (μg)

 

 

Conc.(ng/μl)

 

 

Vhoriyamu (μl)

 

 

Kuchena

 

gDNA

≥ 2

≥ 20

 

≥ 20

 

Kuderedza kuderera uye kusvibiswa

 

 

 

Kuyerera Kwebasa Rebasa

sampuli yekuendesa

Muenzaniso wekutumira

Pilot experiment

Kutorwa kweDNA

Kugadzirira Raibhurari

Kuvakwa kweraibhurari

Sequencing

Sequencing

Data analysis

Data analysis

数据上传-01

Kuendesa data


  • Zvakapfuura:
  • Zvinotevera:

  •  Bioinformatics

    mufananidzo1

    1.Genome-wide DNA methylation distribution

    3(1)

    2.Annotation pane yakanyanya-methylated CGI nzvimbo

    4(1)

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