Diki RNA sequencing-Illumina
Features
● Kukura kusarudzwa kweRNA kusati kwagadzirira raibhurari
● Ongororo yeBioinformatic yakanangana nekufembera kwe miRNA uye zvinangwa zvavo
Basa Rinobatsira
●Comprehensive bioinformatics analysis:kugonesa kuzivikanwa kwezvose zvinozivikanwa uye novel miRNAs, kuzivikanwa kwemiRNAs tarisiro uye inoenderana inoshanda annotation uye kupfumisa neakawanda dhatabhesi (KEGG, GO)
●Rigorous Quality Control: Isu tinoshandisa epakati kudzora mapoinzi pamatanho ese, kubva kumuenzaniso uye kugadzirira raibhurari kune kutevedzana uye bioinformatics.Uku kutarisisa kwakadzama kunovimbisa kuburitswa kwezvinogara zvemhando yepamusoro mhedzisiro.
●Post-Sales Support: Kuzvipira kwedu kunowedzera kupfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi nguva.Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.
●Hunyanzvi Hwakawanda: iine rekodhi rekodhi yekuvhara zvakabudirira pamusoro peakawanda sRNA mapurojekiti anofukidza anopfuura zana emarudzi munzvimbo dzakasiyana dzekutsvagisa, timu yedu inounza hupfumi hweruzivo kupurojekiti yega yega.
Sample Zvinodiwa uye Delivery
| Library | Platform | Yakakurudzirwa data | Data QC |
| Saizi yakasarudzwa | Illumina SE50 | 10M-20M inoverengwa | Q30≥85% |
Sample Zvinodiwa:
Nucleotides:
| Conc.(ng/μl) | Mari (μg) | Kuchena | Kuperera |
| ≥ 80 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel. | RIN≥6.5; 5.0≥28S/18S≥1.0; kukwirira kwakadzika kana kusina kwekutanga |
● Zvirimwa:
Mudzi, Dzinde kana Petal: 450 mg
Shizha kana Mbeu: 300 mg
Muchero: 1.2 g
● Mhuka:
Mwoyo kana Ura: 450 mg
Viscera kana Brain: 240 mg
Tsandanyama: 600 mg
Mabhonzo, Bvudzi kana Ganda: 1.5g
● Arthropods:
Zvipembenene: 9g
Crustacea: 450 mg
● Ropa rose: 2 machubhu
● Masero: 106 masero
● Serum uye Plasma:6 mL
Yakakurudzirwa Sample Delivery
Container:
2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)
Sample labeling: Boka+ replicate eg A1, A2, A3;B1, B2, B3...
Shipment:
1.Dry-ice: Samples dzinoda kuiswa mumabhegi uye kuvigwa muoma-ice.
2.RNAstable tubes: RNA samples inogona kuomeswa muRNA stabilization tube (eg RNAstable®) uye inotumirwa mukupisa kwekamuri.
Kuyerera Kwebasa Rebasa
Kuedza kugadzira
Muenzaniso wekutumira
RNA kubviswa
Kuvakwa kweraibhurari
Sequencing
Data analysis
Mushure mekutengesa masevhisi
Bioinformatics
Kuzivikanwa kwe miRNA: chimiro uye kudzika
Kusiyana kwekutaura kwe miRNA - hiearchical clustering
Inoshanda tsinhiro yechinangwa chemiRNA yakaratidzwa zvakasiyana
Ongorora kufambira mberi kwekutsvagisa kunofambiswa neBMKGene 'sRNA kutevedzana masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.
Chen, H. et al.(2023) 'Zvirwere zvehutachiona zvinodzivisa saponin biosynthesis uye photosynthesis muPanax notoginseng', Plant Physiology uye Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al.(2023) 'Chirimwa FYVE domain-ine protein FREE1 inoshamwaridzana nemicroprocessor zvikamu kudzvinyirira miRNA biogenesis', EMBO inoshuma, 24 (1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. nevamwe.(2023) 'The MicroRNA Ame-Bantam-3p Inodzora Larval Pupal Development neTargeting the Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) muHoneybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al.(2018) 'Integrated Analysis yeMiRNA uye Genes Inobatanidza Nehutano Hwenyama Inoratidza kuti Gga-MiR-140-5p Inobata Intramuscular Fat Deposition muHuku', Cellular Physiology uye Biochemistry, 46 (6), mapeji 2421-2433.doi: 10.1159/000489649.
