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Single-nucleus RNA Sequencing
Iko kufambira mberi mukutora sero rimwe chete uye nzira yekuvaka raibhurari yega-inosanganiswa neyepamusoro-kuburikidza kutevedzana inobvumira gene expression zvidzidzo pasero-ne-sero.Iyo inogonesa yakadzama uye yakazara sisitimu yekuongorora pane yakaoma cell populations, iyo inodzivirira zvakanyanya masking yehterogeneity yavo nekutora mavhareji emasero ese.
Zvisinei, mamwe masero haana kukodzera kuitwa mu-single-sero suspension, saka dzimwe nzira dzekugadzirira sampuli - nucleus extraction kubva kumatissue inodiwa, kureva kuti, nucleus inotorwa zvakananga kubva mumatishu kana sero uye inogadzirirwa mu-single-nucleus suspension for single- cell sequencing.
BMK inopa gumi × Genomics Chromium TM yakavakirwa imwe-sero RNA sequencing sevhisi.Iyi sevhisi yakashandiswa zvakanyanya muzvidzidzo pazvidzidzo zvine chekuita nechirwere, senge immune cell kusiyanisa, tumor heterogeneity, kukura kwetishu, nezvimwe.
Spatial transcriptome chip: 10 × Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Spatial transcriptomics inomira kumberi kwehunyanzvi hwesainzi, ichipa simba vaongorori kuti vatarise mumatanho ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epakati.Pakati pemapuratifomu akasiyana, BMKGene yakagadzira iyo BMKManu S1000 Spatial Transcriptome Chip, ichizvikudza.kugadzirisa kwakawedzerwaye5µM, ichisvika kune subcellular renji, uye inogonesamulti-level resolution settings.Iyo S1000 chip, ine nzvimbo dzinosvika mamirioni maviri, inoshandisa madiki madiki akaturikidzwa nemabhero akaiswa ane spatially barcoded capture probes.Iyo cDNA raibhurari, yakafumiswa ne spatial barcode, inogadzirwa kubva kuS1000 chip uye inozoteedzerwa paIllumina NovaSeq papuratifomu.Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa.Iyo BMKManu S1000 chip yakasarudzika hunhu iri mukusiyana kwayo, ichipa akawanda-level resolution marongero ayo anogona kunyatso gadziridzwa kune akasiyana matishu uye mazinga ezveruzivo.Uku kuchinjika kunoisa chip sechisarudzo chakatanhamara chezvakasiyana zve spatial transcriptomics zvidzidzo, kuve nechokwadi chakanyatso sangana chepakati neruzha rushoma.
Uchishandisa iyo BMKManu S1000 chip uye mamwe epamhepo transcriptomics tekinoroji, vatsvakurudzi vanogona kuwana kunzwisisa kuri nani kwesangano renzvimbo yemasero uye kuwirirana kwema molecular kunoitika mukati mematishu, zvichipa ruzivo rwakakosha mumaitiro ari pasi pebiological process mune dzakasiyana siyana minda, kusanganisira. oncology, neuroscience, developmental biology, immunology uye botanical zvidzidzo.
Platform: BMKManu S1000 chip uye Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics tekinoroji yekucheka-kumucheto inobvumira vaongorori kuti vaongorore maitiro ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epamhepo.Imwe puratifomu ine simba mune ino domain ndeye 10x Genomics Visium yakasanganiswa neIllumina kutevedzana.Iyo musimboti we10X Visium iri pane yakasarudzika chip ine yakasarudzika nzvimbo yekubata inoiswa zvikamu zvetishu.Iyi nzvimbo yekubata ine barcoded mavara, imwe neimwe inoenderana nenzvimbo yakasarudzika mukati mematishu.Iwo akabatwa maRNA mamorekuru kubva munyama anobva anyorwa neakasarudzika mamorekuru identifiers (UMIs) panguva yekudzokesa kunyorwa maitiro.Aya mabarcoded spots uye UMIs anogonesa chaiyo spatial mepu uye quantification yegeneti kutaura pane imwechete-sero resolution.Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa.Nekushandisa iyi Spatial Transcriptomics tekinoroji, vaongorori vanogona kuwana nzwisiso yakadzama yekurongeka kwenzvimbo yemasero uye kupindirana kwakaoma kwema molecular kunoitika mukati mematishu, zvichipa ruzivo rwakakosha mumatanho ari pasi pemaitiro ezvipenyu mundima dzakawanda, kusanganisira oncology, neuroscience, developmental biology, immunology. , uye zvidzidzo zvebotanical.
Platform: 10X Genomics Visium uye Illumina NovaSeq
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Yakazara-Kureba mRNA Sequencing-Nanopore
Nepo NGS-yakavakirwa mRNA sequencing inoshanda sechombo chakasiyana-siyana cheiyo gene expression quantification, kuvimba kwayo nekuverenga kupfupi kunodzikisira kushanda kwayo mukuongorora kwakaoma kwezvinyorwa.Nanopore sequencing, kune rumwe rutivi, inoshandisa tekinoroji yekuverenga kwenguva refu, ichigonesa kutevedzana kwehurefu hwakazara mRNA zvinyorwa.Iyi nzira inogonesa kuongororwa kwakadzama kweimwe nzira yekubatanidza, gene fusions, poly-adenylation, uye quantification yemRNA isoforms.
Nanopore sequencing inotsamira pane nanopore single-morekuru real-time magetsi masaini.Inotungamirwa nemapuroteni emota, DNA ine tambo mbiri inosunga kune nanopore mapuroteni akaiswa mubiofilm, achisununguka sezvainopfuura nemu nanopore chiteshi pasi pekusiyana kwevoltage.Iwo akasiyana masaini emagetsi anogadzirwa nemabhesi akasiyana paDNA strand anoonekwa uye akaiswa muchikamu-chaiyo-nguva, kufambisa kwakaringana uye kuenderera nucleotide sequencing.Iyi nzira yekuvandudza inokunda zvipimo zvekuverenga kwenguva pfupi uye inopa chikuva chine simba chekuomesesa genomic kuongororwa, inosanganisira yakaoma transcriptomic zvidzidzo.
Platform: Nanopore Promethion P48
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Yakazara-kureba mRNA kutevedzana -PacBio
Nepo NGS-yakavakirwa mRNA sequencing iri chishandiso chinogoneka chekuenzanisa gene kutaura, kuvimba kwayo nekuverenga kupfupi kunodzora kushandiswa kwayo mukuomarara kwekuongorora.PacBio sequencing (Iso-Seq), kune rumwe rutivi, inoshandisa tekinoroji yekuverenga kwenguva refu, ichigonesa kutevedzana kwehurefu hwakazara mRNA zvinyorwa.Iyi nzira inogonesa kuongororwa kwakadzama kweimwe nzira splicing, gene fusions uye poly-adenylation kunyangwe isiri iyo yekutanga sarudzo yegene expression quantification, nekuda kwehuwandu hwedata hunodiwa.
PacBio sequencing tekinoroji inotsamira pane imwe-morekuru, chaiyo-nguva (SMRT) kutevedzana, ichipa mukana wakasiyana mukutora yakazara-kureba mRNA zvinyorwa.Iyi nzira yekuvandudza inosanganisira kushandiswa kwezero-mode waveguides (ZMWs), matsime ane microfabricated anogonesa kutarisa-nguva chaiyo yeDNA polymerase chiitiko panguva yekutevedzana.Mukati meaya maZMWs, PacBio's DNA polymerase inogadzira tambo inopindirana yeDNA, ichigadzira kuverenga kwakareba kunosvika kuzere kwezvinyorwa zvemRNA.PacBio oparesheni muCircular Consensus sequencing (CCS) modhi inosimudzira kurongeka nekudzokorodza kuteedzera mamorekuru mamwe chete.Iwo akagadzirwa HiFi anoverenga ane huchokwadi hunofananidzwa neNGS, ichienderera mberi ichipa kune yakazara uye yakavimbika ongororo yeakaoma transcriptomic maficha.Platform: PacBio Sequel II
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Eukaryotic mRNA Sequencing-Illumina
mRNA sequencing inogonesa kukwenenzverwa kwezvese mRNA zvinyorwa mukati memasero pasi pemamiriro chaiwo.Iyi tekinoroji yekucheka-kumucheto inoshanda sechishandiso chine simba, kuvheneka akaomesesa gene kutaura profiles, majini zvimiro, uye mamorekuru maitiro ane chekuita neakasiyana maitiro ebhayoloji.Yakagamuchirwa zvakanyanya mukutsvagisa kwakakosha, kuongororwa kwekiriniki, uye kukura kwezvinodhaka, kutevedzana kwemRNA kunopa manzwisisiro mukati mekuoma kwemaserura dynamics uye genetic regulation.
Platform: Illumina NovaSeq X
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Non-Reference based mRNA Sequencing-Illumina
mRNA sequencing inogonesa kukwenenzverwa kwezvese mRNA zvinyorwa mukati memasero pasi pemamiriro chaiwo.Iyi tekinoroji yekucheka-kumucheto inoshanda sechishandiso chine simba, kuvheneka akaomesesa gene kutaura profiles, majini zvimiro, uye mamorekuru maitiro ane chekuita neakasiyana maitiro ebhayoloji.Yakagamuchirwa zvakanyanya mukutsvagisa kwakakosha, kuongororwa kwekiriniki, uye kukura kwezvinodhaka, kutevedzana kwemRNA kunopa manzwisisiro mukati mekuoma kwemaserura dynamics uye genetic regulation.
Platform: Illumina NovaSeq X
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Yakareba isiri-coding kutevedzana-Illumina
Marefu asiri ekukodha maRNA (lncRNAs) maRNA akareba kupfuura mazana maviri nucleotides ane mashoma ekukodha anogona uye ari akakosha zvinhu mukati isiri-coding RNA.Inowanikwa mune nucleus uye cytoplasm, aya maRNA anoita basa rakakosha mune epigenetic, transcriptal, uye post-transcriptal regulation, zvichisimbisa kukosha kwavo mukugadzira maserura uye mamorekuru maitiro.LncRNA sequencing chishandiso chine simba mukusiyanisa Cell, Ontogenesis, uye Zvirwere zvevanhu.
Platform: Illumina NovaSeq
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Diki RNA sequencing-Illumina
Madiki eRNA (sRNA) mamorekuru, anowanzo pasi pe200 nucleotides pakureba, anosanganisira microRNAs (miRNAs), maRNA madiki anopindira (siRNAs), uye piwi-interacting RNAs (piRNAs).Pakati peaya, miRNAs, yakatenderedza 20-24 nucleotides kureba, inonyanya kukosha pamabasa avo akakosha ekutonga mumaserura akasiyana maitiro.Nematishu-chaiwo uye nhanho-chaiwo maitiro ekutaura, miRNAs inoratidza kuchengetedza kwepamusoro pamarudzi akasiyana.
Platform: Illumina NovaSeq
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circRNA sequencing-Illumina
Circular RNA sequencing (circRNA-seq) ndeyekufananidza uye kuongorora maRNA akatenderera, kirasi yemamorekuru eRNA anoumba zvishwe zvakavharwa nekuda kwezviitiko zvisiri zvecanonical splicing, zvichipa RNA idzi nekuwedzera kugadzikana.Nepo mamwe ma circRNA akaratidzwa kuita semicroRNA sponges, sequering microRNAs uye kuvadzivirira kubva pakudzora vavanoda mRNAs, mamwe ma circRNA anogona kudyidzana nemapuroteni, modulate gene expression, kana kuita mabasa mumaserura.circRNA kutaura kwekuongorora kunopa ruzivo mumabasa ekutonga emamorekuru aya uye kukosha kwawo mumaitiro akasiyana-siyana emasero, nhanho dzekusimudzira, uye mamiriro echirwere, zvichipa kunzwisisa kwakadzama kwekuoma kwemutemo weRNA mumamiriro ekutaura kwejeni.
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Yese transcriptome sequencing - Illumina
Yese transcriptome sequencing inopa yakazara nzira yekunyora akasiyana mamorekuru eRNA, anosanganisira ese macoding (mRNA) uye asiri-coding RNAs (lncRNA, circRNA, uye miRNA).Iyi tekinoroji inobata iyo yakazara transcriptome yemaseru chaiwo panguva yakapihwa, ichibvumira kunzwisisa kwakazara kwemaserura maitiro.Iyo inozivikanwawo se "yakazara RNA kutevedzana," ine chinangwa chekufumura akaomesesa ekutonga network padanho rekunyora, ichigonesa kuongororwa kwakadzama sekukwikwidza endo native RNA (ceRNA) uye yemubatanidzwa RNA kuongororwa.Izvi zvinoratidzira danho rekutanga rekuita hunhu hwekuita, kunyanya mukufumura mataneti ekudzora anosanganisira circRNA-miRNA-mRNA-yakavakirwa ceRNA kusangana.
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Prokaryotic mRNA kutevedzana
mRNA sequencing inogonesa kukwenenzverwa kwezvese mRNA zvinyorwa mukati memasero pasi pemamiriro chaiwo.Iyi tekinoroji yekucheka-kumucheto inoshanda sechishandiso chine simba, kuvheneka akaomesesa gene kutaura profiles, majini zvimiro, uye mamorekuru maitiro ane chekuita neakasiyana maitiro ebhayoloji.Yakagamuchirwa zvakanyanya mukutsvagisa kwakakosha, kuongororwa kwekiriniki, uye kukura kwezvinodhaka, kutevedzana kwemRNA kunopa manzwisisiro mukati mekuoma kwemaserura dynamics uye genetic regulation.Yedu prokaryotic mRNA sampuli yekugadzirisa yakagadzirirwa prokaryotic transcriptomes, inosanganisira rRNA kupera uye inotungamira raibhurari kugadzirira.
Platform: Illumina NovaSeq X
