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Single-nucleus RNA Sequencing
Iko kufambira mberi mukutora sero rimwe chete uye nzira yekuvaka raibhurari yega-inosanganiswa neyepamusoro-kuburikidza kutevedzana inobvumira gene expression zvidzidzo pasero-ne-sero.Iyo inogonesa yakadzama uye yakazara sisitimu yekuongorora pane yakaoma cell populations, iyo inodzivirira zvakanyanya masking yehterogeneity yavo nekutora mavhareji emasero ese.
Zvisinei, mamwe masero haana kukodzera kuitwa mu-single-sero suspension, saka dzimwe nzira dzekugadzirira sampuli - nucleus extraction kubva kumatissue inodiwa, kureva kuti, nucleus inotorwa zvakananga kubva mumatishu kana sero uye inogadzirirwa mu-single-nucleus suspension for single- cell sequencing.
BMK inopa gumi × Genomics Chromium TM yakavakirwa imwe-sero RNA sequencing sevhisi.Iyi sevhisi yakashandiswa zvakanyanya muzvidzidzo pazvidzidzo zvine chekuita nechirwere, senge immune cell kusiyanisa, tumor heterogeneity, kukura kwetishu, nezvimwe.
Spatial transcriptome chip: 10 × Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Spatial transcriptomics inomira kumberi kwehunyanzvi hwesainzi, ichipa simba vaongorori kuti vatarise mumatanho ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epakati.Pakati pemapuratifomu akasiyana, BMKGene yakagadzira iyo BMKManu S1000 Spatial Transcriptome Chip, ichizvikudza.kugadzirisa kwakawedzerwaye5µM, ichisvika kune subcellular renji, uye inogonesamulti-level resolution settings.Iyo S1000 chip, ine nzvimbo dzinosvika mamirioni maviri, inoshandisa madiki madiki akaturikidzwa nemabhero akaiswa ane spatially barcoded capture probes.Iyo cDNA raibhurari, yakafumiswa ne spatial barcode, inogadzirwa kubva kuS1000 chip uye inozoteedzerwa paIllumina NovaSeq papuratifomu.Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa.Iyo BMKManu S1000 chip yakasarudzika hunhu iri mukusiyana kwayo, ichipa akawanda-level resolution marongero ayo anogona kunyatso gadziridzwa kune akasiyana matishu uye mazinga ezveruzivo.Uku kuchinjika kunoisa chip sechisarudzo chakatanhamara chezvakasiyana zve spatial transcriptomics zvidzidzo, kuve nechokwadi chakanyatso sangana chepakati neruzha rushoma.
Uchishandisa iyo BMKManu S1000 chip uye mamwe epamhepo transcriptomics tekinoroji, vatsvakurudzi vanogona kuwana kunzwisisa kuri nani kwesangano renzvimbo yemasero uye kuwirirana kwema molecular kunoitika mukati mematishu, zvichipa ruzivo rwakakosha mumaitiro ari pasi pebiological process mune dzakasiyana siyana minda, kusanganisira. oncology, neuroscience, developmental biology, immunology uye botanical zvidzidzo.
Platform: BMKManu S1000 chip uye Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics tekinoroji yekucheka-kumucheto inobvumira vaongorori kuti vaongorore maitiro ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epamhepo.Imwe puratifomu ine simba mune ino domain ndeye 10x Genomics Visium yakasanganiswa neIllumina kutevedzana.Iyo musimboti we10X Visium iri pane yakasarudzika chip ine yakasarudzika nzvimbo yekubata inoiswa zvikamu zvetishu.Iyi nzvimbo yekubata ine barcoded mavara, imwe neimwe inoenderana nenzvimbo yakasarudzika mukati mematishu.Iwo akabatwa maRNA mamorekuru kubva munyama anobva anyorwa neakasarudzika mamorekuru identifiers (UMIs) panguva yekudzokesa kunyorwa maitiro.Aya mabarcoded spots uye UMIs anogonesa chaiyo spatial mepu uye quantification yegeneti kutaura pane imwechete-sero resolution.Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa.Nekushandisa iyi Spatial Transcriptomics tekinoroji, vaongorori vanogona kuwana nzwisiso yakadzama yekurongeka kwenzvimbo yemasero uye kupindirana kwakaoma kwema molecular kunoitika mukati mematishu, zvichipa ruzivo rwakakosha mumatanho ari pasi pemaitiro ezvipenyu mundima dzakawanda, kusanganisira oncology, neuroscience, developmental biology, immunology. , uye zvidzidzo zvebotanical.
Platform: 10X Genomics Visium uye Illumina NovaSeq
