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Illumina uye BGI
Illumina sequencing tekinoroji, yakavakirwa paSequencing by Synthesis (SBS), innovation yakambundirwa pasi rose yeNGS, ine basa rekugadzira inopfuura makumi mapfumbamwe muzana yedata renyika.Nheyo yeSBS inosanganisira kufungidzira fluorescently yakanyorwa reversible terminators sezvo dNTP yega yega inowedzerwa, uye yakazonamirwa kubvumira kubatanidzwa kweiyo chigadziko chinotevera.Nezvose zvina zvinodzoreredzwa zveterminator-yakasungwa dNTPs dziripo mune yega yega kutevedzana kutenderera, makwikwi echisikigo anoderedza kubatanidza kusarerekera.Iyi tekinoroji inosiyana-siyana inotsigira ese ari maviri-averengeka uye maviri-ekupedzisira-maraibhurari, achiitira kune akasiyana genomic application.Illumina sequencing's high-throughput kugona uye nemazvo inoisa iyo senheyo yekona mukutsvagisa genomics, ichipa simba masayendisiti kuburitsa hunyoro hwema genomes ane ruzivo rusingaenzaniswi uye kugona.
DNBSEQ, yakagadziridzwa neBGI, imwe yehunyanzvi hweNGS tekinoroji yakakwanisa kudzikisira pasi mutengo wekutevedzana uye kuwedzera kubuda.Kugadzirira kwemaraibhurari eDNBSEQ kunosanganisira kupatsanurwa kweDNA, kugadzirira ssDNA uye kutenderedza denderedzwa amplification kuti uwane iyo DNA nanoballs (DNB).Izvi zvinobva zvatakurwa panzvimbo yakasimba uye zvichizoteedzerwa necombinatorial Probe-Anchor Synthesis (cPAS).
Yedu yakafanogadzirwa raibhurari yekuteedzera sevhisi inogonesa vatengi mukugadzirira kwavo kuteedzera maraibhurari kubva kwakasiyana masosi (mRNA, yakazara genome, amplicon, pakati pevamwe).Mushure mezvo, maraibhurari aya anogona kutumirwa kunzvimbo dzedu dzekuteedzera kudzora kwemhando uye kutevedzana muIllumina kana BGI mapuratifomu.
