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BSA
Bulked Segregant Analysis chikuva chine nhanho imwe-yakajairwa ongororo uye ongororo yepamberi ine yakagadziridzwa paramende.BSA inzira inoshandiswa kukurumidza kuona phenotype yakabatana genetic mamaki.Main workflow yeBSA ine: 1. kusarudza mapoka maviri evanhu vane phenotypes anopesana zvakanyanya;2. kubatanidza DNA, RNA kana SLAF-seq(Yakagadziridzwa neBiomarker) yevanhu vese kuti vaumbe zvikamu zviviri zveDNA;3. kudoma mitsara yakasiyana maererano nereferensi genome kana pakati, 4. kufanotaura matunhu akabatanidzwa neED uye SNP-index algorithm;5. Kuongorora kwekushanda uye kupfumisa pamajini munzvimbo dzevamiriri, nezvimwewo. Kunyanya kucherwa kwepamusoro mu data kusanganisira genetic marker screening uye primer design inowanikwawo.
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Amplicon (16S/18S/ITS)
Amplicon (16S/18S/ITS) papuratifomu inogadzirwa nemakore ehunyanzvi mukuongororwa kweprojekiti yakasiyana-siyana, iyo ine yakajairwa ongororo yekuongorora uye ongororo yemunhu: ongororo yekutanga inovhara iyo huru yekuongorora zvirimo yezvino hutachiona tsvakiridzo, iyo yekuongorora yemukati yakapfuma uye yakazara, uye mhedzisiro yekuongorora inoratidzwa muchimiro chemishumo yeprojekiti;Izvo zvemukati ongororo yemunhu zvakasiyana.Masamples anogona kusarudzwa uye ma paramita anogona kusetwa achichinjika zvinoenderana neiyo yekutanga ongororo rondedzero uye chinangwa chekutsvagisa, kuti uwane zvinodiwa nemunhu.Windows operating system, iri nyore uye inokurumidza.
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Evolutionary Genetics
Evolutionary genetics ibasa rakazara rekutevedzana rakagadzirirwa kupa dudziro yakazara paruzivo rwekushanduka kwezvinhu zvakapihwa zvichibva mukusiyana kwemajini, kusanganisira SNPs, InDels, SVs uye CNVs.Inopa kuongorora kwese kwakakosha kunodiwa pakutsanangura shanduko yekushanduka-shanduka uye maumbirwo emarudzi evanhu, zvakadai sehuwandu hwevanhu, kusiyana kwemajini, hukama hwefilogeny, nezvimwewo. Iinewo zvidzidzo pamusoro pekuyerera kwemajini, izvo zvinopa simba kufungidzira kwehuwandu hwevanhu hunobudirira, nguva yekusiyana.
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Kuenzanisa Genomics
Comparative genomics zvinoreva kuenzanisa kutevedzana kwegenome yakazara uye zvimiro zvemarudzi akasiyana.Chirango ichi chine chinangwa chekuburitsa kushanduka kwemhando, basa remajini, nzira yekudzora majini padanho rejenome nekuratidza kutevedzana zvimiro uye zvinhu zvakachengetedza kana kusiyanisa marudzi akasiyana.Yakajairika yekuenzanisa genomics yekudzidza inosanganisira ongororo mumhuri yejini, kusimukira kwekushanduka, kudzokorodza kwegenome yakazara, kudzvanywa kwekusarudza, nezvimwe.
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Evolutionary Genetics
Huwandu hwevanhu uye evolutionary genetic analysis platform inovambwa pahwaro hwechiitiko chikuru chakaunganidzwa mukati meBMK R&D timu kwemakore.Icho chishandiso chine hushamwari mushandisi kunyanya kune vaongorori vasiri hukuru mune bioinformatics.Iyi puratifomu inogonesa evolutionary genetics ine chekuita nekuongororwa kwekutanga kunosanganisira phylogenetic muti kuvaka, yekubatanidza disequilibrium kuongororwa, genetic kusiyana kwekuongorora, yakasarudzika kutsvaira, kuongororwa kwehukama, PCA, ongororo yehuwandu hwevanhu, nezvimwe.
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Hi-C yakavakirwa Genome Assembly
Hi-C inzira yakagadzirirwa kubata chromosome kumisikidzwa nekubatanidza kuongorora kudhuze-kwakavakirwa kupindirana uye yakakwirira-kuburikidza sequencing.Kusimba kwekudyidzana uku kunofungidzirwa kuve kwakabatana zvisina kunaka nekureba kwemuviri pamakromosomes.Naizvozvo, Hi-C data inogona kutungamira kuunganidzwa, kurongeka uye kutungamira kweakaunganidzwa akateedzana mugwaro rejenome uye kusimudzira iwo pane imwe nhamba yemakromosomes.Iyi tekinoroji inopa simba kugungano re chromosome-level genome pasina huwandu hwevanhu-based genetic mepu.Yese genome imwe inoda Hi-C.
Platform: Illumina NovaSeq Platform / DNBSEQ
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Chirimwa/Mhuka De Novo Genome Sequencing
De Novokutevedzana kunoreva kuvakwa kwejenome rerudzi rwemhando yose vachishandisa matekinoroji ekutevedzanisa, semuenzaniso PacBio, Nanopore, NGS, zvichingodaro, pasina genome rereferensi.Kuvandudzwa kunoshamisa mukuverenga kureba kwechizvarwa chechitatu kutevedzana matekinoroji kwakaunza mikana mitsva mukuunganidza majenomes akaoma, akadai seaya ane heterozygosity yakakwira, chiyero chepamusoro chenzvimbo dzinodzokororwa, mapolyploid, nezvimwewo. kugadzirisa zvinhu zvinodzokororwa, matunhu ane abnormal GC zvirimo uye mamwe matunhu akaomarara.
Platform: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq Platform
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Kutevedzana Kwemunhu Yese Exome
Whole exome sequencing (WES) inoonekwa senzira inodhura-inoshanda yekuteedzera nzira yekuziva shanduko dzinokonzera chirwere.Kunyangwe maexons achingotora angangoita 1.7% yegenome yese, inomiririra chimiro chese mapuroteni anoshanda zvakananga.Mune genome yemunhu, zvakashumwa kuti inopfuura 85% yezvirwere zvinoenderana nekuchinja kunoitika munharaunda yeprotein coding.
BMKGENE inopa yakazara uye inochinjika yemunhu yese exome sequencing masevhisi ane akasiyana exon ekutora nzira dziripo dzekuzadzisa zvinangwa zvakasiyana zvekutsvaga.
Platform: Illumina NovaSeq Platform
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, kunyanya pahuwandu hwevanhu, inhanho inokosha muzvidzidzo zvekubatana kwemajini, izvo zvinopa genetic nheyo yekushanda kwemajini ekutsvaga, evolutionary analysis, etc. Panzvimbo yekudzika kwese genome re-sequencing, kuderedza kumiririra genome sequencing (RRGS). ) inounzwa kuti ideredze mutengo wekuteedzana pasample, uku uchichengetedza kugona kwakanaka pakuwana genetic marker.Izvi zvinowanzo wanikwa nekubvisa chimedu chezvirambidzo mukati mehukuru hwakapihwa, iyo inonzi yakaderedzwa yekumiririra raibhurari (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) inzira yekuzvigadzira yega yeSNP genotyping ine kana isina referensi genome.
Platform: Illumina NovaSeq Platform -
Illumina uye BGI
Illumina sequencing tekinoroji, yakavakirwa paSequencing by Synthesis (SBS), innovation yakambundirwa pasi rose yeNGS, ine basa rekugadzira inopfuura makumi mapfumbamwe muzana yedata renyika.Nheyo yeSBS inosanganisira kufungidzira fluorescently yakanyorwa reversible terminators sezvo dNTP yega yega inowedzerwa, uye yakazonamirwa kubvumira kubatanidzwa kweiyo chigadziko chinotevera.Nezvose zvina zvinodzoreredzwa zveterminator-yakasungwa dNTPs dziripo mune yega yega kutevedzana kutenderera, makwikwi echisikigo anoderedza kubatanidza kusarerekera.Iyi tekinoroji inosiyana-siyana inotsigira ese ari maviri-averengeka uye maviri-ekupedzisira-maraibhurari, achiitira kune akasiyana genomic application.Illumina sequencing's high-throughput kugona uye nemazvo inoisa iyo senheyo yekona mukutsvagisa genomics, ichipa simba masayendisiti kuburitsa hunyoro hwema genomes ane ruzivo rusingaenzaniswi uye kugona.
DNBSEQ, yakagadziridzwa neBGI, imwe yehunyanzvi hweNGS tekinoroji yakakwanisa kudzikisira pasi mutengo wekutevedzana uye kuwedzera kubuda.Kugadzirira kwemaraibhurari eDNBSEQ kunosanganisira kupatsanurwa kweDNA, kugadzirira ssDNA uye kutenderedza denderedzwa amplification kuti uwane iyo DNA nanoballs (DNB).Izvi zvinobva zvatakurwa panzvimbo yakasimba uye zvichizoteedzerwa necombinatorial Probe-Anchor Synthesis (cPAS).
Yedu yakafanogadzirwa raibhurari yekuteedzera sevhisi inogonesa vatengi mukugadzirira kwavo kuteedzera maraibhurari kubva kwakasiyana masosi (mRNA, yakazara genome, amplicon, pakati pevamwe).Mushure mezvo, maraibhurari aya anogona kutumirwa kunzvimbo dzedu dzekuteedzera kudzora kwemhando uye kutevedzana muIllumina kana BGI mapuratifomu.
