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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
ChIP-Seq inopa genome-wide profiling yeDNA tarisiro yekuchinja kwehistone, zvinyorwa zvinyorwa, uye mamwe mapuroteni ane DNA.Inosanganisa kusarudzwa kwechromatin immuno-precipitation (ChIP) kuti idzore chaiwo mapuroteni-DNA complexes, nesimba reinotevera-chizvarwa sequencing (NGS) yekumusoro-kuburikidza kutevedzana kweiyo yakadzoserwa DNA.Pamusoro pezvo, nekuti maprotein-DNA complexes anodzoserwa kubva kumaseru mhenyu, nzvimbo dzinosunga dzinogona kuenzaniswa mumhando dzakasiyana dzesero uye matishu, kana pasi pemamiriro akasiyana.Zvikumbiro zvinotangira kubva pakunyorwa kwegwaro kuenda kunzira dzekusimudzira kune nzira dzechirwere uye nekupfuura.
Platform: Illumina NovaSeq Platform
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Metagenomic Sequencing -NGS
Metagenome (Metagenome) inoreva muunganidzwa wezvakakwana zvezvipenyu zvemusanganiswa wenharaunda yezvipenyu, zvakaita semetagenome yezvakatipoteredza, metagenome yevanhu, zvichingodaro. Ine majenomes ezvese tupukanana tunorimika uye tusingarimike.Metagenomic sequencing is a molecular tool inoshandiswa kuongorora zvakasanganiswa genomic zvinhu zvakatorwa kubva kune zvakatipoteredza samples, izvo zvinopa ruzivo rwakadzama mumhando dzakasiyana-siyana uye kuwanda, chimiro chehuwandu hwevanhu, hukama hwe phylogenetic, kushanda kwemajini uye correlation network nemamiriro ekunze.
Platform:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics is a molecular tool inoshandiswa kuongorora zvakasanganiswa genomic zvinhu zvakatorwa kubva kune zvakatipoteredza samples, izvo zvinopa ruzivo rwakadzama mukusiyana kwemarudzi uye kuwanda, chimiro chehuwandu hwevanhu, phylogenetic ukama, kushanda kwemajini uye correlation network nezvakatipoteredza, etc. Nanopore sequencing platforms ichangobva kuiswa kune zvidzidzo zvemetagenomic.Kuita kwayo kwakatanhamara mukureba kwekuverenga kwakanyanya kukwidziridzwa pasi perukova metagenomic ongororo, kunyanya metagenome musangano.Kutora mabhenefiti ekuverenga-kureba, Nanopore-yakavakirwa metagenomic kudzidza inokwanisa kuwana yakawanda inoenderera gungano ichienzanisa nepfuti-pfuti metagenomics.Yakaburitswa kuti Nanopore-yakavakirwa metagenomics yakabudirira kuburitsa yakazara uye yakavharwa mabhakitiriya genomes kubva kumamicrobiomes (Moss, EL, et. al,Nature Biotech, 2020)
Platform:Nanopore PromethION P48
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Yese genome bisulfite kutevedzana
DNA methylation panzvimbo yechishanu mu cytosine (5-mC) ine simba guru pakutaura kwemajini uye kushandiswa kwemasero.Abnormal methylation maitiro akave akabatanidzwa neanoverengeka mamiriro uye zvirwere, senge kenza.WGBS yave chiyero chegoridhe chekudzidza genome-wide methylation pane imwechete base resolution.
Platform: Illumina NovaSeq Platform
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Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira Kuburikidza Sequencing (ATAC-seq)
ATAC-seq inzira yepamusoro-yekutevedzana yekuongorora genome-wide chromatin accessibility, iyo yakakosha kupasi rose epigenetic control ye gene expression.Sequencing adapters inoiswa munzvimbo dzakavhurika chromatin ne hyperactive Tn5 transposase.Mushure mePCR kukwidziridzwa, raibhurari yekutevedza inogadzirwa.Ese akavhurika chromatin matunhu anogona kuwanikwa pasi peiyo nzvimbo-yenguva mamiriro, kwete chete inogumira kune inosunga nzvimbo dzechinyorwa chinhu, kana imwe histone yakagadziridzwa dunhu.
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16S/18S/ITS Amplicon Sequencing-PacBio
Iyo subunit pa16S uye 18S rRNA ine ese akachengetedzeka zvakanyanya uye hyper-anochinjika matunhu ndeye yakakwana molecular zvigunwe zvekuzivikanwa kweprokaryotic uye eukaryotic zvipenyu.Tichitora mukana wekutevedzana, aya amplicon anogona kunangwa zvichibva pazvikamu zvakachengetwa uye matunhu anochinja-chinja anogona kunyatsoratidzwa hutachiona huchibatsira muzvidzidzo zvinofukidza hutachiona hwekusiyana kwekuongorora, taxonomy, phylogeny, nezvimwe. Imwe-morekuru real-time(SMRT) ) kutevedzana kwePacBio papuratifomu inogonesa kuwana kuverenga kwakareba kwakaringana, uko kunogona kuvhara maamplicon akazara (approx. 1.5 KB).Maonero akatambanudzwa enzvimbo yemajini akasimudzira zvakanyanya kugadziriswa kwezvipenyu mumutsara wemabhakitiriya kana fungi.
Platform:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing inovavarira kuburitsa phylogeny, taxonomy, uye huwandu hwemhando munharaunda yemicrobial nekuongorora PCR zvigadzirwa zvekuchengetedza imba mamakisi ane zvese zvinokurukurwa uye hypervariable zvikamu.Kuunzwa kweaya akakwana mamorekuru zvigunwe naWoeses et al, (1977) anopa simba rekuzvimiririra-isina microbiome profiling.Sequencing ye16S (mabhakitiriya), 18S (fungi) uye Internal transcribed spacer (ITS, fungi) inobvumira kuzivikanwa kwemarudzi ari maviri akawanda pamwe chete neasingawanzo uye asingazivikanwe marudzi.Iyi tekinoroji yave chinhu chinoshandiswa zvakanyanya uye chikuru chishandiso mukuona mutsauko wekuumbwa kwehutachiona munzvimbo dzakasiyana siyana, senge muromo wemunhu, ura, tsvina, nezvimwe.
Platform:Illumina NovaSeq Platform
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Bhakitiriya uye Fungal Yese Genome Re-Sequencing
Bhakitiriya uye fungal yese genome re-sequencing chishandiso chakakosha kupedzisa genomes yeanozivikanwa bhakitiriya uye fungi, pamwe nekuenzanisa akawanda genomes kana kumepu genomes ezvipenyu zvitsva.Izvo zvakakosha zvikuru kutevedza genomes rose rebhakitiriya uye fungi kuitira kuti ugadzire mareferensi chaiwo genomes, kuita microbial identification uye zvimwe kuenzanisa genome zvidzidzo.
Platform:Illumina NovaSeq Platform
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PacBio-Yakazara-kureba 16S/18S/ITS Amplicon Sequencing
Amplicon (16S/18S/ITS) papuratifomu inogadzirwa nemakore ehunyanzvi mukuongororwa kweprojekiti yakasiyana-siyana, iyo ine yakajairwa ongororo yekuongorora uye ongororo yemunhu: ongororo yekutanga inovhara iyo huru yekuongorora zvirimo yezvino hutachiona tsvakiridzo, iyo yekuongorora yemukati yakapfuma uye yakazara, uye mhedzisiro yekuongorora inoratidzwa muchimiro chemishumo yeprojekiti;Izvo zvemukati ongororo yemunhu zvakasiyana.Masamples anogona kusarudzwa uye ma paramita anogona kusetwa achichinjika zvinoenderana neiyo yekutanga ongororo rondedzero uye chinangwa chekutsvagisa, kuti uwane zvinodiwa nemunhu.Windows operating system, iri nyore uye inokurumidza.
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PacBio-Yakazara-kureba Transcriptome (Isiri Reference)
Kutora Pacific Bioscience (PacBio) Isoform yekuteedzera data seyekuisa, iyi App inokwanisa kuona yakazara-yakareba zvinyorwa zvinoteedzana (pasina kuungana).Nekuita mepu yakazara-yakareba sequence inopesana nereferenzi genome, zvinyorwa zvinogona kugadziridzwa neanozivikanwa majini, zvinyorwa, matunhu ekodhi, nezvimwewo. Muchiitiko ichi, kunyatsozivikanwa kwemRNA zvimiro, seimwe nzira yekubatanidza, nezvimwewo, inogona kuwanikwa.Kuongororwa kwakabatana neNGS transcriptome sequencing data inogonesa chirevo chakazara uye chakanyanya kurongeka mukutaura padanho rekunyora, iro rinobatsira zvakanyanya kudzika kwemusiyano kutaura uye ongororo yekushanda.
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Yakaderedzwa Representation Bisulfite Sequencing (RRBS)
Tsvagiridzo yeDNA methylation yagara iri nyaya inopisa mukutsvaga kwechirwere, uye yakabatana zvakanyanya nekutaura kwemajini uye pheno-typic maitiro.RRBS inzira yakarurama, inoshanda uye ine mari yeDNA methylation research.Kufumiswa kweanosimudzira uye CpG chitsuwa matunhu ne enzymatic cleavage (Msp I), yakasanganiswa neBisulfite sequencing, inopa yakakwirira resolution DNA methylation yekuona.
Platform: Illumina NovaSeq Platform
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Prokaryotic mRNA kutevedzana
mRNA sequencing inogonesa kukwenenzverwa kwezvese mRNA zvinyorwa mukati memasero pasi pemamiriro chaiwo.Iyi tekinoroji yekucheka-kumucheto inoshanda sechishandiso chine simba, kuvheneka akaomesesa gene kutaura profiles, majini zvimiro, uye mamorekuru maitiro ane chekuita neakasiyana maitiro ebhayoloji.Yakagamuchirwa zvakanyanya mukutsvagisa kwakakosha, kuongororwa kwekiriniki, uye kukura kwezvinodhaka, kutevedzana kwemRNA kunopa manzwisisiro mukati mekuoma kwemaserura dynamics uye genetic regulation.Yedu prokaryotic mRNA sampuli yekugadzirisa yakagadzirirwa prokaryotic transcriptomes, inosanganisira rRNA kupera uye inotungamira raibhurari kugadzirira.
Platform: Illumina NovaSeq X
