Spatial transcriptomics inomira kumberi kwehunyanzvi hwesainzi, ichipa simba vaongorori kuti vatarise mumatanho ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epakati.Pakati pemapuratifomu akasiyana, BMKGene yakagadzira iyo BMKManu S1000 Spatial Transcriptome Chip, ichizvikudza.kugadzirisa kwakawedzerwaye5µM, ichisvika kune subcellular renji, uye inogonesamulti-level resolution settings.Iyo S1000 chip, ine nzvimbo dzinosvika mamirioni maviri, inoshandisa madiki madiki akaturikidzwa nemabhero akaiswa ane spatially barcoded capture probes.Iyo cDNA raibhurari, yakafumiswa ne spatial barcode, inogadzirwa kubva kuS1000 chip uye inozoteedzerwa paIllumina NovaSeq papuratifomu.Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa.Iyo BMKManu S1000 chip yakasarudzika hunhu iri mukusiyana kwayo, ichipa akawanda-level resolution marongero ayo anogona kunyatso gadziridzwa kune akasiyana matishu uye mazinga ezveruzivo.Uku kuchinjika kunoisa chip sechisarudzo chakatanhamara chezvakasiyana zve spatial transcriptomics zvidzidzo, kuve nechokwadi chakanyatso sangana chepakati neruzha rushoma.
Uchishandisa iyo BMKManu S1000 chip uye mamwe epamhepo transcriptomics tekinoroji, vatsvakurudzi vanogona kuwana kunzwisisa kuri nani kwesangano renzvimbo yemasero uye kuwirirana kwema molecular kunoitika mukati mematishu, zvichipa ruzivo rwakakosha mumaitiro ari pasi pebiological process mune dzakasiyana siyana minda, kusanganisira. oncology, neuroscience, developmental biology, immunology uye botanical zvidzidzo.
Platform: BMKManu S1000 chip uye Illumina NovaSeq