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Metagenomic Sequencing -NGS
Metagenome (Metagenome) inoreva muunganidzwa wezvakakwana zvezvipenyu zvemusanganiswa wenharaunda yezvipenyu, zvakaita semetagenome yezvakatipoteredza, metagenome yevanhu, zvichingodaro. Ine majenomes ezvese tupukanana tunorimika uye tusingarimike.Metagenomic sequencing is a molecular tool inoshandiswa kuongorora zvakasanganiswa genomic zvinhu zvakatorwa kubva kune zvakatipoteredza samples, izvo zvinopa ruzivo rwakadzama mumhando dzakasiyana-siyana uye kuwanda, chimiro chehuwandu hwevanhu, hukama hwe phylogenetic, kushanda kwemajini uye correlation network nemamiriro ekunze.
Platform:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics is a molecular tool inoshandiswa kuongorora zvakasanganiswa genomic zvinhu zvakatorwa kubva kune zvakatipoteredza samples, izvo zvinopa ruzivo rwakadzama mukusiyana kwemarudzi uye kuwanda, chimiro chehuwandu hwevanhu, phylogenetic ukama, kushanda kwemajini uye correlation network nezvakatipoteredza, etc. Nanopore sequencing platforms ichangobva kuiswa kune zvidzidzo zvemetagenomic.Kuita kwayo kwakatanhamara mukureba kwekuverenga kwakanyanya kukwidziridzwa pasi perukova metagenomic ongororo, kunyanya metagenome musangano.Kutora mabhenefiti ekuverenga-kureba, Nanopore-yakavakirwa metagenomic kudzidza inokwanisa kuwana yakawanda inoenderera gungano ichienzanisa nepfuti-pfuti metagenomics.Yakaburitswa kuti Nanopore-yakavakirwa metagenomics yakabudirira kuburitsa yakazara uye yakavharwa mabhakitiriya genomes kubva kumamicrobiomes (Moss, EL, et. al,Nature Biotech, 2020)
Platform:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Iyo subunit pa16S uye 18S rRNA ine ese akachengetedzeka zvakanyanya uye hyper-anochinjika matunhu ndeye yakakwana molecular zvigunwe zvekuzivikanwa kweprokaryotic uye eukaryotic zvipenyu.Tichitora mukana wekutevedzana, aya amplicon anogona kunangwa zvichibva pazvikamu zvakachengetwa uye matunhu anochinja-chinja anogona kunyatsoratidzwa hutachiona huchibatsira muzvidzidzo zvinofukidza hutachiona hwekusiyana kwekuongorora, taxonomy, phylogeny, nezvimwe. Imwe-morekuru real-time(SMRT) ) kutevedzana kwePacBio papuratifomu inogonesa kuwana kuverenga kwakareba kwakaringana, uko kunogona kuvhara maamplicon akazara (approx. 1.5 KB).Maonero akatambanudzwa enzvimbo yemajini akasimudzira zvakanyanya kugadziriswa kwezvipenyu mumutsara wemabhakitiriya kana fungi.
Platform:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing inovavarira kuburitsa phylogeny, taxonomy, uye huwandu hwemhando munharaunda yemicrobial nekuongorora PCR zvigadzirwa zvekuchengetedza imba mamakisi ane zvese zvinokurukurwa uye hypervariable zvikamu.Kuunzwa kweaya akakwana mamorekuru zvigunwe naWoeses et al, (1977) anopa simba rekuzvimiririra-isina microbiome profiling.Sequencing ye16S (mabhakitiriya), 18S (fungi) uye Internal transcribed spacer (ITS, fungi) inobvumira kuzivikanwa kwemarudzi ari maviri akawanda pamwe chete neasingawanzo uye asingazivikanwe marudzi.Iyi tekinoroji yave chinhu chinoshandiswa zvakanyanya uye chikuru chishandiso mukuona mutsauko wekuumbwa kwehutachiona munzvimbo dzakasiyana siyana, senge muromo wemunhu, ura, tsvina, nezvimwe.
Platform:Illumina NovaSeq Platform
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Bhakitiriya uye Fungal Yese Genome Re-Sequencing
Bhakitiriya uye fungal yese genome re-sequencing chishandiso chakakosha kupedzisa genomes yeanozivikanwa bhakitiriya uye fungi, pamwe nekuenzanisa akawanda genomes kana kumepu genomes ezvipenyu zvitsva.Izvo zvakakosha zvikuru kutevedza genomes rose rebhakitiriya uye fungi kuitira kuti ugadzire mareferensi chaiwo genomes, kuita microbial identification uye zvimwe kuenzanisa genome zvidzidzo.
Platform:Illumina NovaSeq Platform
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Metatranscriptome Sequencing
Metatranscriptome sequencing inozivisa gene expression yemicrobes (zvese eukaryotes uye prokaryotes) mukati mezvakatipoteredza zvechisikigo (kureva ivhu, mvura, gungwa, tsvina, uye ura.) Kunyanya, masevhisi aya anobvumidza iwe kuwana yakazara gene kutaura profiling yenharaunda dzakaoma hutachiona, taxonomic analysis. yezvipenyu, mashandiro ekusimudzira ongororo yemajini anoratidzwa akasiyana, nezvimwe.
Platform: Illumina NovaSeq Platform
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Fungal Genome
Biomarker Technologies inopa genome ongororo, yakanaka genome uye pene-yakazara genome yefungal zvichienderana nechinangwa chekutsvaga.Genome sequencing, kuungana uye kushanda annotation inogona kuwanikwa nekubatanidza Inotevera-chizvarwa kutevedzana + Yechitatu chizvarwa kutevedzana kuti iwane yakakwirira-level genome ungano.Hi-C tekinoroji inogona zvakare kushandiswa kufambisa genome kusangana pakromosome level.
Platform:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Utachiona Hwakazara Genome
Biomarker Technologies inopa kutevedzana sevhisi pakuvaka yakazara genome yebhakitiriya ine zero gap.Kufambiswa kukuru kwebhakitiriya kupedzisa kuvaka genome kunosanganisira kutevedzana kwechizvarwa chechitatu, kuungana, dhizaini inoshanda uye yepamberi bioinformatic ongororo inozadzisa zvakanangana zvinangwa zvekutsvagisa.Kurongeka kwakadzama kwebhakitiriya genome inopa simba kuburitsa maitiro akakosha ari pasi pemaitiro avo ebhayoloji, ayo anogona kupawo kukosha kwakakosha kutsvagurudzo dzegenomic mumhando dzepamusoro eukaryotic.
Platform:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
