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Biomarker Technologies Yakapupurira 31 YakabudiriraDe novoGenome Research muna 2021

Muna 2021, BMKGENE yakapupurira 31 De novo genome tsvakiridzo yakabudirira kuburitswa mumagazini akanyanya-inobata ane yakazara mhedzisiro zvinhu pamusoro pe320. 15 yezvinyorwa zvakanyorwa pamwe 4 vavo vakabatanidzwa sevanyori vekutanga neBMKGENE.

Mushure mekutanga kwegore ra2022, pakatove nezvinyorwa zviviri zvekutsvagisa zvakaburitswa mujenari "Natural Genetics" uye "Molecular Plant" zvichiteerana.Ndivo, "Two divergent haplotypes kubva kune yakanyanya heterozygous lychee genome inoratidzira yakazvimirira zviitiko zvekumba zvekutanga uye kunonoka-kukura cultivars" (Lychee genome research, yakaitwa neCollege of Horticulture, South China Agricultural yunivhesiti, uye vesainzi vabatsiri, rakabudiswa pa Natural Genetics. ), uye "Genome sequences yemarudzi mashanu eSitopsis eAegilops uye kwakabva polyploid gorosi B-subgenome" (Five Sitopsis species genome, inotungamirirwa naProfessor Bao Liu's research team yeNortheast Normal University.).Tichadzokorora zvakare zvinyorwa zviviri izvi togovana nevaverengi vedu.

Ikozvino, ngatitarisei kune zvakanakisa zvinyorwa zvekutsvagisa zvakaburitswa muna 2021 zvakanyorwa neBMK uye yedu yakabatana zvivakwa.

Plant Genome - Kubudirira pamhando dzakasiyana-siyana.

1.Musangano wemhando yepamusoro wegenome unoratidza rye genomic maitiro uye agronomically akakosha majini.

Nzvimbo Yakabatanidzwa: Henan Agricultural University

Nyaya: Natural Genetics

Impact Factor: 38.31

Muchirongwa ichi, genome yeWeining rye, yemhando yepamusoro yeChinese rye, yakatevedzana.Iwo akaunganidzwa contigs (7.74 Gb) akaverengera 98.47% yeanofungidzirwa genome saizi (7.86 Gb), aine 93.67% yemacontigs (7.25 Gb) akaiswa kune manomwe makromosomes.Zvinhu zvinodzokororwa zvakaumba 90.31% ye genome yakaungana.Kumwezve ongororo yegungano reWeining kwakaratidza kujeka kutsva pakudzokororwa kwegenome-wide gene uye maitiro azvo pane starch biosynthesis majini, kurongeka kwechimiro cheprolamin loci yakaoma, maratidziro egeneti ari pasi pekutanga kwekutanga hunhu uye kuisa mudzimba-kwakabatana chromosomal nzvimbo uye loci mu rye.Iyi genome sequence inovimbisa kukurumidza genomic uye kubereka zvidzidzo mu rye uye zvine hukama zviyo zvirimwa.

2.Rose isina prickle: genomic manzwisisiro akabatanidzwa nekunyorova kugadzirisa

Nzvimbo Yakabatanidzwa: Kunming Institute of botany, Chinese Academy of Sciences

Chinyorwa: National Science Ongororo

Impact Factor: 17.273

Muchirongwa ichi, sampuli dze'Basye's Thornless' (BT, mbeu isina prickle yeRosa wichuraiana), 'Old Blush' (OB, muvambi wegenotype mukukura kwemaruva), mahybrids avo eF1 neBCF1 akaunganidzwa.Uye yemhando yepamusoro referensi genome musangano wakagadzirwa kuti uone ma genetic zvinhu zvine chekuita ne stem prickle development.Saizi yegenome inenge 530.6 Mb.Kuti uone kunaka kweiyo genome yakaunganidzwa, kuongororwa senge genetic mepu kuenzanisa, BUSCO, NGS inoverenga kusanganisa, kuenzanisa neOB haplotype, kuteedzera base kukanganisa mwero wekutonga uye genome-wide LTR Assembly Index kukosha cheki (LAI = 20.03) yakaitwa.Tsvagiridzo iyi inoburitsa maitiro akaomarara enhaka uye mashandiro edzinde prickles uye akatipa hwaro uye novel zviwanikwa zvekudzidza rose biology uye kuchera mamorekuru mamaki ane chekuita nehunhu hwakakosha.

3.Whole-Genome Sequence yeSynthesized Allopolyploids muCucumis Inoburitsa Muono muGenome Evolution yeAllopolyploidization

Nzvimbo Yakabatanidzwa: Nanjing Agricultural University

Nyaya: Advanced Sayenzi

Impact Factor: 16.801

Ichi chidzidzo chakashuma genome yemhando yepamusoro yeallotetraploid yakagadzirwa yakawanikwa pakashandiswa interspecific hybridization pakati pecucumber (C. sativus, 2n = 14) nemhando dzayo dzesango (C. hystrix, 2n = 24) uye kudzokorora kwekromosome yakatevera, inova yekutanga. yakanyatso kutevedzana synthetic allopolyploid.Kuungana kweiyo genome kwakashandisa mafambiro e "PacBio+BioNano+Hi-C+Illumina" kutevedzana, kwakakonzera saizi yegenome ye530.8Mb uye contig N50 = 6.5Mb.Kuverenga kwakagoverwa kune 19 pseudochromosomes uye subgenomes.Mhedzisiro yacho yakaratidza kuti kusanganiswa, kwete kudzokororwa kwegenome, kunokonzeresa kuwanda kwekuchinja kwegenomic mune zvese zvenyukireya uye cp genomes.Yakaratidza kuti iyo yakagadziriswa heterozygosity inopa C. × hytivus nekuwedzera kunetseka kugadzirisa.Mhedzisiro yacho inopa ruzivo rutsva muchirimwa chepolyploidy evolution uye inopa nzira inotarisirwa yekuberekesa zvirimwa zvichauya.

4.Comparative Genome Inoongorora Highlight Transposon Mediated Genome Kuwedzera uye Evolutionary Architecture ye3D Genomic Folding muCotton.

Nzvimbo Yakabatanidzwa: Huazhong Agricultural University

Nyaya: Molecular Biology uye Evolution

Impact Factor: 16.242

Ichi chirongwa chakashandisa Nanopore Sequencing kuunganidza genome yemhando nhatu dzedonje dzinoti: Gossypium rotundifolium (K2, genome size = 2.44Gb, contigN50 = 5.33Mb), G. arboreum (A2, genome size = 1.62Gb, 1.62Gb, contig16), uye 1. G. raimondii (D5, genome size = 0.75Gb, contigN50 = 17.04 Gb).Pamusoro pe99% yeese matatu genomes akaunganidzwa kuburikidza neHi-C.Mhedzisiro yeBUSCO ongororo ndeye 92.5%, 93.9% uye 95.4% zvichiteerana.Nhamba idzi dzese dzakaratidza kuti matatu egungano genomes are reference-grade.Kuenzanisa genome ongororo yakanyorwa zvinyorwa zvemutsara-zvakananga TE kukwidziridzwa zvichibatsira kune yakakura genome saizi misiyano.Ichi chidzidzo chinopa chiedza pamusoro pebasa re transposon-mediated genome kuwedzera mukushanduka kwepamusoro-soro chromatin chimiro mumiti.

5.Kusangana kweChromosome-scale uye kuongororwa kwechirimwa chebiomass Miscanthus lutarioriparius genome

Yakabatanidzwa Nzvimbo: CAS Center yekunakisa mune molecular Plant Sayenzi

Nyaya: Nature Communications

Impact Factor: 14.912

Ichi chirongwa chakashuma chromosome-scale musangano weMiscanthus lutarioriparius genome nekubatanidza Oxford Nanopore kutevedzana uye Hi-C tekinoroji.Musangano we2.07Gb unovhara 96.64% yegenome, ine contig N50 ye1.71 Mb.Inenge 94.30% yezvese kutevedzana kwakaiswa mu19 pseudochromosomes.Kuburikidza nekuenzanisa nekutevedzana kweBAC, kuongororwa kweLAI, kuongorora kweBUSCO, kuunganidzazve data reNGS, kuunganidzwazve kwedata retranscriptome, genome yakaongororwa seyemhando yepamusoro uye kuenderera mberi.Mabviro eAllotetraploid yeM. lutarioriparius inosimbiswa uchishandisa centromeric satellite inodzokorora.Tandem duplicate majini eM. lutarioriparius anoshanda akafumiswa kwete chete maererano nemhinduro yekushushikana, asi cell wall biosynthesis.Gene duplicates pamwe inobata basa muC4 photosynthesis uye inobatsira kuMiscanthus C4 photosynthesis pakudziya kwakadzika.Tsvagiridzo iyi yakapa chirevo chakakosha chekudzidza miti isingaperi.

6.A chromosome-level Camptotheca acuminata genome assembly inopa ruzivo pamusoro pekushanduka-shanduka kwe camptothecin biosynthesis.

Nzvimbo Yakabatanidzwa: Sichuan University

Nyaya: Nature Communications

Impact Factor: 14.912

Ichi chirongwa chakashuma yemhando yepamusoro, chromosome-level C. acuminata genome assembly, ine genome size ye414.95Mb uye contingN50 1.47Mb.Takaona kuti C. acuminata inosangana nekuzvimiririra kudzokororwa kwejenome rose uye majini akawanda anobva mairi ane chekuita necamptothecin biosynthesis.Kusiyana kwebasa reLAMT gene uye kushanduka kwakanaka kwemajini maviri eSLAS, naizvozvo, zvose zvakabatsira zvikuru ku camptothecin biosynthesis muC. acuminata.Mhedzisiro yacho yakasimbisa kukosha kwekusangana kwemhando yepamusoro yegenome pakuona shanduko yemajini mukushanduka kwekutanga kwechipiri metabolite.

7.Allele-defined genome inoratidza biallelic kusiyana panguva yekushanduka kwecassava

Nzvimbo Yakabatanidzwa: Chinese Academy yeTropical Agricultural Sayenzi

Nyaya: Molecular Plant

Impact Factor: 13.162

Ichi chirongwa chakaunganidza referensi genome yecassava ine contigN50 1.1Mb ichishandisa Pacific Biosciences (PacBio) yekuteedzera chikuva.Mushure mekuongororwa neBUSCO, LAI index, uye yakakwirira-density genetic mepu, iyo yakaunganidzwa genome inosimbiswa serefence-giredhi.Matunhu asina basa akaonekwa uye contigs akasungirirwa pa18 pseudochromosomes vachishandisa Hi-C link.Iyi yemhando yepamusoro uye yakatsanangurwa referensi genome yemufarinya yakakosha pakuziva akasiyana bi-alleles pahomologous chromosomes, zvichibvumira kuongorora kusiyanisa uye kutaura kutonga kwebi-alleles uye iwo ari pasi peiyo evolutionary kutyaira masimba.Yakafambisa nzira itsva dzekuberekesa mucassava nezvimwe zvirimwa zvine heterozygous.

8. Genomic manzwisisiro ekukura nekukurumidza kwepaulownias uye kuumbwa kwePaulownia varoyi 'broom.

Yakabatanidzwa Nzvimbo: Henan Agricultural yunivhesiti

Nyaya: Molecular Plant

Impact Factor: 13.162

Ichi chirongwa chakaunganidza chemhando yepamusoro chenyukireya genome yePaulownia fortunei, 511.6 Mb muhukuru, iine 93.2% yekutevedzana iri anchod kune makumi maviri epseudochromosomes.Yakakwirira photosynthetic kunyatsoshanda kunowanikwa kuburikidza nekubatanidza C3 photosynthesis uye crassulacean acid metabolism nzira, iyo inogona kunge yakakonzera kukurumidza kukurumidza kukura tsika yepaulownia miti.Kuwedzera genome sequencing yePaWB phytoplasma, yakasanganiswa nekuongorora kwekushanda, yakaratidza kuti muitiro wePaWB-SAP54 unobata zvakananga naPaulownia PfSPLa, izvo zvinokonzeresa kuderedzwa kwePfSPLa nenzira yeubiquitin-mediated uye inotungamira pakuumbwa kwemutsvairo wevaroyi.Iyo data yakapa ruzivo rwakakosha mubiology ye paulownias uye nzira yekudzora yekuumbwa kwePaWB.

Mhuka genome - Manzwisisiro akadzama ekushanduka kwemarudzi

1.Genome yeNautilus pompilius inovhenekera shanduko yeziso uye biomineralization.

Yakabatanidzwa Nzvimbo: South China Sea Institute of Oceanology, CAS

Journal: Natural Ecology & evolution

Impact Factor: 15.462

Ichi chirongwa chakaburitsa genome yakazara yeNautilus pompilius.Iyo ine minimalist genome pakati peakateedzana cephalopods, iri 730.58Mb ine contigN50 = 1.1Mb.Iyo BUSCO yekuongorora mhedzisiro ndeye 91.31%.Yakasanganiswa ne transcriptome, proteome, gene mhuri uye phylogenetic kuongororwa, iyi genome yakapa chirevo chakakosha pane cephalopod innovations, senge pinhole ziso uye biomineralization.Tsvagiridzo yakaratidza kuti kukuvadzwa pakukwana kweHox gene cluster kunogona kunge kwakabatana nekunyangarika kwegomba reMollusks.Zvakakosha, kuwanda kwema genomic hunyanzvi hunosanganisira kurasikirwa kwemajini, kudzikamisa kwakazvimirira uye kuwedzera kwemhuri dzemajini uye netiweki yadzo yakabatana inogona kuumba shanduko yeziso re nautilus pinhole.Iyo nautilus genome yakaita sosi yakakosha yekuvaka patsva mamiriro ekushanduka-shanduka uye genomic hutsva hunoumba cephalopods iripo.

2.Seadragon genome analysis inopa nzwisiso mune yayo phenotype uye bonde kutsunga locus

Yakabatanidzwa Nzvimbo: South China Sea Institute of Oceanology, CAS

Nyaya: Sayenzi Kufambira mberi

Impact Factor: 14.132

Iyi purojekiti de novo-yakatevedzana echirume neechikadzi genomes eiyo common seadragon (Phyllopteryx taeniolatus) nemhando dzayo dzine hukama, alligator pipefish (Syngnathoides biaculeatus).Saizi yegenome yePhyllopteryx taeniolatus is ~659 Mb(♂)uye ~663 Mb(♀), ine contigN50 ye10.0Mb ne12.1mb.saizi yegenome yePhyllopteryx taeniolatus i637 Mb(♂)uye ~648 Mb(♀), ine contigN50 ye18.0Mb ne21.0Mb.Kuburikidza nekuongorora phylogenetic, seadragon yakajairika nealligator pipefish ihanzvadzi yemutero yeSyngnathinae, uye yakatsaukana kunosvika 27.3 Ma apfuura.Zvinyorwa zvinyorwa kubva kutsvarakadenga, mashizha-akafanana nemaappendages, anoratidza kuti seti yemajini inowanzobatanidzwa mukuvandudzwa kwekupedzisira yakasarudzwa pamwe nekupfumisa zvinyorwa zvekugona kugadzirisa matishu uye majini ekudzivirira immune.Iyo yekuisa bonde-inosarudzira nzvimbo inokodha yemurume-yakananga amhr2y gene yakagovaniswa neyakajairika seadragon uye alligator pipefish yakaonekwa.Iyi purojekiti yakapa humbowo hwakakosha hwezvidzidzo zvekushanduka-shanduka.


Nguva yekutumira: Sep-19-2022

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