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Nanopore-based Yakazara-kureba Transcriptome Sequencing

Nanopore sequencing inozvisiyanisa kubva kune mamwe mapuratifomu ekutevedzana, pakuti nucleotides inoverengwa zvakananga pasina DNA synthesis uye inogadzira kuverenga kwenguva refu pamakumi e kilobases.Izvi zvinopa simba rakananga kuverenga-kunze kweazere-kureba zvinyorwa uye kugadzirisa zvinonetsa muzvidzidzo zvezvinyorwa.

√ Yakaderera kutevedzana-chaiwo kurerekera

√ Yakazara-kureba kuverenga kwecDNA kune yemajini maitiro ezvidzidzo

√ Dhata shoma inodiwa kuvhara nhamba imwechete yezvinyorwa

√ Kuzivikanwa kweakawanda isoforms pajini

√ Kuratidzira kwehuwandu pane isoform-level

nanopore-1

Yakajairika Bioinformatics Pipeline

Yakazara-kureba-trans-bix-1024x254

Dzazvino Dzakabudirira Nyaya neBiomarker Technologies

1. Ongororo yekuenzanisa yezvinyorwa zvehurefu hwakazara zvinoratidza Gnetum luofuense stem development dynamics.

Journal: Frontiers muGenetics

Rakabudiswa: Kurume 2021

Keywords: MinION |Alternative splicing |APA |lncRNA |Zvinyorwa zvinotaridzwa zvakasiyana

Verenga zvizere-zvinyorwa

2. Unidirectional kufamba kwemaRNA madiki kubva panhungirwa kuenda kumidzi mune interspecific heterografts

Nyaya: Zvisikwa Zvirimwa

Rakabudiswa: Ndira 2021

Keywords: Nanopore |Ilumina |hurefu hwakazara mbozha mRNA yekuona

3. Yakazara-yakareba transcriptome kuongororwa kwemidzi yeasparagus inoratidza iyo molecular nzira yekushivirira kwemunyu kunokonzerwa ne arbuscular mycorrhizal fungi.

Journal: Environmental uye Yekuedza Botany

Rakabudiswa: Ndira 2021

Keywords: MinION |Zvinyorwa zvakasiyana-siyana |Transcription factors |Alternative splicing

4. Physiological Characteristic Shanduko uye Yakazara-Kureba Transcriptome yeRose (Rosa chinensis) Midzi uye Mashizha muKupindura Kusanaya Stress.

Journal: Plant uye Cell Physiology

Rakabudiswa: Gumiguru 2020

Keywords: PromethION |Zvinyorwa zvakasiyana-siyana |Functional prediction |Spliced ​​isoforms |TF uye lncRNA

5. Kuongorora uye kuenzaniswa kwakazara kwePacBio uye nanopore-based RNA sequencing yeArabidopsis transcriptome.

Journal: Plant Method

Rakabudiswa: Kubvumbi 2020

Keywords: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |Genome alignment |Transcript identification |Alternative splicing |SSR |LncRNA |Isoform quantification

Nhau Nezvakakosha inovavarira kugovera nyaya dzichangoburwa dzakabudirira neBiomarker Technologies, kutora budiriro yesainzi pamwe nemaitiro ane mukurumbira akashandiswa panguva yekudzidza.


Nguva yekutumira: Jan-08-2022

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