GENOME EVOLUTION
Kuenzanisa genome ongororo inosimbisa transposon-mediated genome kuwedzera uye evolutionary architecture ye 3D genomic kupeta mudonje.
Nanopore sequencing |Hi-C |PacBio sequencing |Ilumina |RNA-sequencing |3D genome architecture |Transposon |Kuenzanisa genomics
Muchidzidzo ichi, Biomarker Technologies yakapa rutsigiro rwehunyanzvi paNanopore sequencing, Hi-C uye yakakodzera bioinformatic ongororo.
Abstract
Transposable element (TE) amplification yave ichizivikanwa sesimba rekufambisa rinoyananisa genome saizi yekuwedzera uye shanduko, asi mhedzisiro yekuumba 3D genomic architecture inoramba isingazivikanwe muzvirimwa.Pano, tinoshuma mareferensi-giredhi genome magungano emhando nhatu dzedonje dzakapetwa katatu muhukuru hwegenome, dzinoti.Gossypium rotundifolium(K2),G. arboreum(A2) uyeG. raimondii(D5), uchishandisa Oxford Nanopore Technologies.Kuenzanisa genome inoongorora zvinyorwa zvemutsara-zvakananga TE kukwidziridzwa kunobatsira kune yakakura genome saizi misiyano (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), uye inoratidza kuchengetedzwa kwakachengetedzeka gene content uye synteny hukama pakati pema genomes.Takaona kuti inenge 17% yemajini emagetsi anoratidza shanduko yechromatin pakati pezvinoshanda ("A") uye zvisingashande ("B") makamuri, uye kukwidziridzwa kweTE kwakabatana nekuwedzera kwechikamu cheA compartment munzvimbo dzemajini (~ 7,000 majini. ) muK2 uye A2 hukama neD5.Chete 42% yetopologically asociating domain (TAD) miganhu yakachengetedzwa pakati peatatu genomes.Yedu data inobata ichangoburwa kukwidziridzwa kweTEs zvichitevera kuumbwa kwemutsara-chaiwo miganhu yeTAD.Ichi chidzidzo chinopa chiedza pamusoro pebasa re transposon-mediated genome kuwedzera mukushanduka kwepamusoro-soro chromatin chimiro mumiti.
Nhamba dzakakosha dzegungano rejenome
Mufananidzo.Genome assembly uye ficha tsananguro yeG. rotundifolium (K2)
Nhau Nezvakakosha inovavarira kugovera nyaya dzichangoburwa dzakabudirira neBiomarker Technologies, kutora budiriro yesainzi pamwe nemaitiro ane mukurumbira akashandiswa panguva yekudzidza.
Nguva yekutumira: Jan-05-2022