Kuongorora maitiro ekutaura kwemajini panzvimbo yepakutanga yechinhu chipenyu kwakakosha pakunzwisisa mhando nemashandiro emaseru ayo.Nekudaro, ikozvino spatial transcriptome yekuongorora nzira dzine painogumira senge yakaderera throughput kana kusakwana kugadzirisa.Iyo BMKMANU S1000 Spatial Chip, yakagadziridzwa neBMKMANU, inogona kuona in-situ gene kutaura ruzivo muzvikamu zvakakwana zvetishu pane subcellular resolution, ichigonesa kududzira kwakasiyana kwechimiro chetishu.
Gadzirira kuona chigadzirwa chedu chitsva!Kupemberera kuburitswa kwayo, tiri kupa yakakoshaTenga Ina Tora Imwe Yemaharakusimudzirwa kwenguva shoma chete.
Zvigadzirwa Zvikamu
BMKMANU S1000 spatial chip uye inoenderana reagent kit inosanganisira:
1) Tissue Optimization Kit, ine ma reagents ekugadzirisa tishu (yakanyanya nguva yekuongorora yetishu permeabilization).
2) Gene Expression Kit, ine ma reagents ekutora mRNA mumatishu zvidimbu zveanotevera kugadzirira raibhurari uye kutevedzana.
3) Kutanga Kit: Inosanganisira thermostatic adapta uye transcriptome magnetic separator.
Technical Principle
Iyo chip yakavakirwa pamamicropores uye microbeads, uye mRNA inogona kutorwa in situ kuburikidza neOligo pane microbeads.Iyo Oligo inoumbwa nezvikamu zvina: Read1, Spatial Barcode, UMI, uye Poly(dT)VN.Mushure mekunge matishu akasungirirwa kune chip, mRNA inoburitswa kubva munyama ne permeabilization enzyme.Sezvo mazhinji mRNA 3' anoguma aine Poly-A muswe, anotorwa naOligo nePoly(dT)VN.Mushure meRT-PCR uye cDNA kukwidziridzwa, kugadzirira raibhurari uye kutevedzana, nzvimbo yekutsvagisa nzvimbo inowanikwa kuburikidza neSpatial Barcode.Izvi zvinobvumira kuongororwa kwemajini ekutaura munzvimbo dzepasi uye tishu heterogeneity.
Product Advantages
Applications
Inogona kushandiswa kune dzakawanda nzvimbo dzekutsvagisa biological uye zvekurapa, kusanganisira bundu, chirwere, immunology, uye budiriro biology, zvichibatsira kuwana mabudiro matsva mundima idzi nekuongorora kwekutaura kwenzvimbo pakugadziriswa kwe subcellular.
●Bundu uye Chirwere:
Spatial heterogeneity uye microenvironment yemamota uye zvirwere
Kutanga uye kukura kwemamota uye zvirwere
Kurapwa mhinduro yemamota uye zvirwere
●Developmental Biology
Spatiotemporal atlas yenhengo
Gene regulatory mechanisms panguva yekuvandudza
●Kupindura Kushungurudzika
Biotic stress mhinduro
Abiotic stress mhinduro
●Immunology
Kupindura kwekuzvidzivirira kwemuviri mukuiswa kwenhengo
Immune nzira dzemamota uye zvirwere
Pathogenesis yezvirwere zve autoimmune
●Drug resistance analysis
Nzira dzekudzivisa zvinodhaka
Tsvagiridzo nekuvandudzwa kwemishonga mitsva
Nyaya uye Demo Data
BMKMANU S1000 yakaitwa-yakasimbiswa mumazana emakesi mumhando dzakasiyana dzematishu.
