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Proteomics
Proteomics inosanganisira mashandisirwo ematekinoroji ehuwandu hwehuwandu hwemapuroteni aripo mukati mesero, tishu kana chipenyu.MaProteomics-based tekinoroji anoshandiswa munzvimbo dzakasiyana siyana dzekutsvagisa kwakasiyana sekuonekwa kweakasiyana-siyana anocherekedza mamaki, vanoda kugadzirwa kwejekiseni, kunzwisisa pathogenicity nzira, kuchinjwa kwemaitiro ekupindura kune akasiyana masaini uye kududzira inoshanda mapuroteni nzira muzvirwere zvakasiyana.Parizvino, matekinoroji ehuwandu hweproteomics akakamurwa zvakanyanya kuita TMT, Label Yemahara uye DIA nzira dzehuwandu.
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Metabolomics
Iyo metabolome ndiyo inoguma yakadzika chigadzirwa chejenome uye ine yakazara inokwana yeakaderera-molecular-uremu mamorekuru (metabolites) musero, tishu, kana chipenyu.Metabolomics inovavarira kuyera hupamhi hwemamorekuru madiki mumamiriro ekukurudzira kwemuviri kana mamiriro ezvirwere.Metabolomics methodologies inowira mumapoka maviri akasiyana: metabolomics isina kunanga, ongororo yakazara yeese anoyerwa analytes mumuenzaniso kusanganisira kemikari isingazivikanwe uchishandisa GC-MS/LC-MS, uye yakanangwa metabolomics, kuyerwa kwemapoka akatsanangurwa emakemikari anoratidzwa uye. biochemically annotated metabolites.
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Bulked Segregant analysis
Bulked segregant analysis (BSA) inzira inoshandiswa kukurumidza kuona phenotype yakabatana genetic markers.Kufambiswa kukuru kweBSA kune kusarudza mapoka maviri evanhu vane phenotypes anopokana zvakanyanya, kubatanidza DNA yevanhu vese kuti vaumbe maviri akawanda eDNA, ichizivisa mutsauko wakatevedzana pakati pemadziva maviri.Iyi tekinoroji yakashandiswa zvakanyanya mukuziva ma genetic mamaki akabatana zvakanyanya neanonangwa majini muchirimwa / mhuka genomes.
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DNA / RNA Sequencing - Nanopore Sequencer
ONT sequencing ndeye imwe chete molecule chaiyo-nguva yemagetsi chiratidzo chekuenzanisa tekinoroji yakavakirwa nanopores, iyo yekuteedzera musimboti wepuratifomu imwe neimwe yakafanana.DNA/RNA yakasungwa kaviri inosungirira kune nanoporous protein yakanyudzwa mubiofilm uye ichisununguka pasi pekutungamira kwemota protein, pasi pekuita kwevoltage musiyano kubva kumativi ese ebiofilm, DNA/RNA tambo dzinopfuura nemu nanopore chiteshi protein pane imwe nzvimbo. rate.Nekuda kwekusiyana kwemakemikari ezvigadziko zvakasiyana paDNA / RNA strand, kana chigadziko chimwe chete kana DNA molecule ichipfuura nemu nanopore chiteshi, zvinokonzeresa shanduko yemagetsi akasiyana masaini.Nekuona uye kuenderana nezviratidzo izvi, mhando dzepasi dzinoenderana dzinogona kuverengerwa, uye chaiyo-nguva yekuona yekutevedzana inogona kupedzwa.
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DNA / RNA sequencing -PacBio Sequencer
PacBio sequencing chikuva ipuratifomu yekuverenga kwenguva refu, iyo inozivikanwawo seimwe yeTatu-Generation Sequencing (TGS) matekinoroji.Iyo yakakosha tekinoroji, imwe-morekuru chaiyo-nguva (SMRT), inopa simba chizvarwa chekuverenga nemakumi emakirogiramu kureba.Pachigadziko che "Sequencing-by-Synthesis", single nucleotide resolution inowanikwa neZero-mode waveguide (ZMW), apo chete vhoriyamu shoma pazasi (saiti yemamorekuru synthesis), inovhenekerwa.Pamusoro pezvo, kutevedzana kweSMRT kunonyanya kudzivirira kutevedzana-chaiyo bias muNGS system, mukuti mazhinji ematanho ekukwirisa ePCR haadiwe mukugadzira raibhurari.
Platform: Sequel II, Revio
