Products

DNA / RNA sequencing -PacBio Sequencer

PacBio sequencing chikuva ipuratifomu yekuverenga kwenguva refu, iyo inozivikanwawo seimwe yeTatu-Generation Sequencing (TGS) matekinoroji.Iyo yakakosha tekinoroji, imwe-morekuru chaiyo-nguva (SMRT), inopa simba chizvarwa chekuverenga nemakumi emakirogiramu kureba.Pachigadziko che "Sequencing-by-Synthesis", single nucleotide resolution inowanikwa neZero-mode waveguide (ZMW), apo chete vhoriyamu shoma pazasi (saiti yemamorekuru synthesis), inovhenekerwa.Pamusoro pezvo, kutevedzana kweSMRT kunonyanya kudzivirira kutevedzana-chaiyo bias muNGS system, mukuti mazhinji ematanho ekukwirisa ePCR haadiwe mukugadzira raibhurari.

Platform: Sequel II, Revio

Taura nesu

Service Details

Demo Results

Features

Maitiro maviri ekutevedzana paPacBio sequencer: Inoenderera Yakareba Kuverenga (CLR) uye Circular Consensus Read (CCS)

Sequencing Mode

Library Size

Theoretical DataGoho (Per Cell)

Single-baseKururama

Applications

CLR

20Kb, 30Kb, zvichingodaro.

80GB kusvika 130GB

Approx.85%

De novo, SV kufona, nezvimwe.

CCS

15-20 KB

14 kusvika 40 Gb/Sero (Sequel II)

70 kusvika 110 Gb/Sero (Revio)

Zvinoenderana nemuenzaniso

Approx.99%

De novo, SNP/Indel/SV kufona, Iso-Seq,

Kuenzanisa kwekuita uye maficha eRevio uye Sequel II

Terms	Sequel II system	Revio system	Wedzera
Higher density	8 miriyoni ZMWs	25 miriyoni ZMWs	3x
Independent matanho	1	4	4x
Nguva pfupi yekumhanya	30 maawa	24 maawa	1.25x
30X HiFi vanhu genomes / gore	88	1,300	15x zvakazara

Basa Rinobatsira

● Kupfuura 8 makore ruzivo paPacBio sequencing chikuva nezviuru zvemapurojekiti akavharwa ane marudzi akasiyana-siyana.

● Yakanyatsoshongedzerwa neazvino PacBio Sequencing mapuratifomu, Revio kuvimbisa zvakakwana kutevedzana kwekupinda.

● Inokurumidza kutendeuka-nguva, goho repamusoro uye data rakanyatsojeka.

● Yakapiwa mumazana ezvinyorwa zvepamusoro zvePacBio-based.

Mienzaniso Zvinodiwa

Muenzaniso Type

Mari

Concentration (Qubit®)

Volume

Kuchena

Vamwe

Genomic DNA

Zvichienderana neData Inodiwa

≥50 ng/μl

≥15μl

OD260/280=1.7-2.2;
OD260/230=1.8-2.5;
Yakajeka peak pa260 nm,hapana kusvibiswa

Concentration inoda kuyerwa neQubit uye Qubit/Nanopore = 0.8-2.5

Zvose RNA

≥1.2μg

≥120 ng/μl

≥15μl

OD260/280=1.7-2.5;
OD260/230=0.5-2.5;hapana kusvibiswa

RIN kukosha ≥7.5

5≥28S/18S≥1

Service Workflow

Kugadzirira kwemuenzaniso

Kuvakwa kweraibhurari

Sequencing

Data analysis

Kuendesa kweprojekiti

Zvakapfuura: OEM mugadziri Mrna Sequencing Service - Yakareba isiri-coding kutevedzana-Illumina - Biomarker

Zvinotevera: Illumina uye BGI

1.In-house Data Kubereka

Data inogadzirwa kubva ku63 CCS maseru (kubva ku26 marudzi)

DATA-PacBio-CCS-15 KB	Avhareji	Max	Min	Median
Goho - zvishoma (Gb)	421.12	544.27	221.38	426.58
Yiled - CCS(Gb)	25.93	38.59	10.86	25.43
Polymerase N50	145,651	175,430	118,118	144,689
Subreads N50	17,509	23,924	12,485	17,584
CCS N50	14,490	19,034	9,876	14,747
Avhareji urefu-Polymerase	67,995	89,379	49,664	66,433
Avhareji urefu-Zvidiki	15,866	21,036	11,657	16,012
Hurefu hweavhareji-CCS	14,489	19,074	8,575	14,655

Dhata inogadzirwa kubva ku16 CLR maseru (kubva ku76 marudzi)

DATA-PacBio-CLR-30Kb	Avhareji	Max	Min	Median
Goho - zvishoma (Gb)	142.20	291.40	50.55	142.49
Polymerase N50	39,456	121,191	15,389	35,231
Subreads N50	28,490	41,012	14,430	29,063
Avhareji urefu-Polymerase	22,063	48,886	8,747	21,555
Avhareji urefu-Zvidiki	17,720	27,225	8,293	17,779

2.Data QC - DemoStatistics pane data goho

Muenzaniso	ccs Inoverenga Num	Zvose ccs Mabhesi (bp)	ccs Inoverenga N50 (bp)	ccs Kureba Kureba (bp)	ccs Yakareba Kuverenga (bp)	subreads Mabhesi (bp)	ccs rate(%)
PB_BMKxxx	3,444,159	54,164,122,586	15,728	15,726	36,110	863,326,330,465	6.27