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Chromatin Immunoprecipitation Sequencing (ChIP-seq)

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Featured Image

ChIP-Seq inopa genome-wide profiling yeDNA tarisiro yekuchinja kwehistone, zvinyorwa zvinyorwa, uye mamwe mapuroteni ane DNA.Inosanganisa kusarudzwa kwechromatin immuno-precipitation (ChIP) kudzoreredza chaiwo mapuroteni-DNA complexes, nesimba reinotevera-chizvarwa sequencing (NGS) yekumusoro-kuburikidza kutevedzana kweiyo yakadzoserwa DNA.Pamusoro pezvo, nekuti maprotein-DNA complexes anodzoserwa kubva kumaseru mhenyu, nzvimbo dzinosunga dzinogona kuenzaniswa mumhando dzakasiyana dzemasero uye matishu, kana pasi pemamiriro akasiyana.Zvikumbiro zvinotangira kubva pakunyorwa kwegwaro kuenda kunzira dzekusimudzira kune nzira dzechirwere uye nekupfuura.

Platform: Illumina NovaSeq Platform

Taura nesu

Service Details

Bioinformatics

Demo Results

Basa Rinobatsira

● Platform: Illumina NovaSeq Platform, PE150

● Rudzi rweraibhurari: 100-500bp isa raibhurari yeDNA (zvinoenderana nekuparadzirwa kwepamusoro)

● Sequencing strategy: Paired-End 150 bp

● Kurudzira kubudiswa kwemashoko: 6 Gb data raw / samples.

Muenzaniso Zvinodiwa

DNA huwandu: ≥ 70 ng, huru yepamusoro ye100 kusvika 500 bp

Sample volume: ≥ 10 μl

OD260/280 = 1.8 kusvika 2.0 pasina kuora kana kusvibiswa kweRNA

Experimental workflow

Kuyerera Kwebasa Rebasa

sampuli yekuendesa

Muenzaniso wekutumira

Kugadzirira Raibhurari

Kuvakwa kweraibhurari

Sequencing

Sequencing

Data analysis

Data analysis

Mushure mekutengesa Services

Mushure mekutengesa masevhisi

Zvakapfuura: Metagenomic Sequencing -NGS

Zvinotevera: Yese transcriptome sequencing - Illumina

1. Peak hupfumi kugovera

2. MA chirongwa chekusiyanisa peak

3.KEGG chirevo

4. GO classification

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