Utachiona Hwakazara Genome
Basa Rinobatsira
● Nzira dzakawanda dzekutevedzana dziripo dzezvinangwa zvekutsvaga zvakasiyana
● Utachiona hwakakwana genome ne 0 Gap yakavimbiswa.
● Ane ruzivo rwakanyanya mukusangana kwemabhakitiriya ejenome uye anopfuura 10,000 akazara genome akaunganidzwa.
● Nyanzvi mushure mekutengesa tekinoroji yetsigiro timu inozadzisa zvakanangana tsvakiridzo zvinodiwa.
Sevhisi Zvinotsanangurwa
| SequencingStrategy | Library | Hunhu hunovimbiswa | Inofungidzirwa kutendeuka-nguva |
| Nanopore 100X + Illumina 50X | Nanopore 20K PE150 | 0 Gap | 30 Mazuva |
| PacBio HiFi 30X | PacBio 10K |
Bioinformatics inoongorora
● Raw data quality control
● Gungano reGenome
● Genome chikamu chekuongorora
● Gene basa rondedzero
● Kuenzanisa genomic kuongorora
Sample Zvinodiwa uye Delivery
Sample Zvinodiwa:
ForDNA zvinyorwa:
| Muenzaniso Type | Mari | Concentration | Kuchena |
| DNA zvinyorwa | ＞ 2 μg | ＞ 20 ng/μl | OD260/280= 1.6-2.5 |
Kune matishu samples:
| Muenzaniso mhando | Yakakurudzirwa kurapwa kwemuenzaniso | Sample kuchengetedza uye kutumira |
| Utachiona | Tarisa mabhakitiriya pasi pemaikorosikopu uye unganidza mabhakitiriya muchikamu chavo cheexponential Shandura tsika yebhakitiriya (ine approx. 3-4.5e9 masero) mu 1.5 kana 2 ml eppendorf.(Ramba uri muchando) Centrifuge chubhu ye1 min pa14000 g kuunganidza mabhakitiriya uye kubvisa supernatant nekungwarira. Nama chubhu uye omesa mabhakitiriya mune mvura nitrogen kweinenge 30 min.Chengetedza chubhu mu -80 ℃ firiji. | Svitsa masampula munitrogen yemvura kwemaawa 3-4 uye chengetedza mune yemvura nitrogen kana -80 dhigirii kusvika kurebesa kwenguva refu.Sample shipping with dry-ice inodiwa. |
Kuyerera Kwebasa Rebasa
Muenzaniso wekutumira
Kuvakwa kweraibhurari
Sequencing
Data analysis
Mushure mekutengesa masevhisi
1.Circos yebhakitiriya genome
2.Coding gene kufanotaura
3.Comparative genomics analysis: phylogenetic tree
