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Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira Kuburikidza Sequencing (ATAC-seq)

Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira throughput Sequencing (ATAC-seq) Featured Image
  • Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira Kuburikidza Sequencing (ATAC-seq)
  • Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira Kuburikidza Sequencing (ATAC-seq)
  • Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira Kuburikidza Sequencing (ATAC-seq)
  • Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira Kuburikidza Sequencing (ATAC-seq)
  • Assay yeTransposase-Inowanikwa Chromatin ine Yakakwira Kuburikidza Sequencing (ATAC-seq)

ATAC-seq inzira yepamusoro-yekutevedzana yekuongorora genome-wide chromatin accessibility, iyo yakakosha kupasi rose epigenetic control ye gene expression.Sequencing adapters inoiswa munzvimbo dzakavhurika chromatin ne hyperactive Tn5 transposase.Mushure mePCR kukwidziridzwa, raibhurari yekutevedza inogadzirwa.Ese akavhurika chromatin matunhu anogona kuwanikwa pasi peiyo nzvimbo-yenguva mamiriro, kwete chete inogumira kune inosunga nzvimbo dzechinyorwa chinhu, kana imwe histone yakagadziridzwa dunhu.


Service Details

Bioinformatics

Demo Results

Basa Rinobatsira

● Comprehensive Analysis: Inyanzvi yekuongorora pombi yekusangana nemhando dzakasiyana-siyana dzekuongorora zvinodiwa;

● Zvakaitika Zvakawanda: Mazana emapurojekiti akapedzwa zvinobudirira kusvika zvino, kusanganisira mhuka nezvirimwa samples.

Sevhisi Zvinotsanangurwa

Platform: Illumina NovaSeq chikuva

Raibhurari mhando: ATAC-seq

Inokurudzirwa kuburitsa data: ≥20M inoverengwa

Muenzaniso Zvinodiwa

Sample type: Matishu, masero mhenyu, kana maseru akaomeswa nechando zvichingodaro

Sero nhamba: ≥ 5×105masero, nhamba chaiyo yesero ndiyo kiyi yekubudirira kwekuyedza;

Kurema kwenyama: ≥ 200mg itsva tishu;

Ropa: ≥ 2 mL

Kuyerera Kwebasa Rebasa

Muenzaniso QC

Kuedza kugadzira

sampuli yekuendesa

Muenzaniso wekutumira

Pilot experiment

Kutorwa kweDNA

Kugadzirira Raibhurari

Kuvakwa kweraibhurari

Sequencing

Sequencing

Data analysis

Data analysis

Mushure mekutengesa Services

Mushure mekutengesa masevhisi

  • Zvakapfuura:
  • Zvinotevera:

    • 表观-02

    1.Heatmap paATAC inoverenga kugovera kuTSS nenzvimbo dziri pedyo (±3 kb)

    3 (1)2.Kuparidzirwa kwekromatin yakazaruka munzvimbo dzakasiyana-siyana dzejenome

    4 (1)

    3.Differential peak kudana pakati pemapoka

    5 (1)

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