Small RNA

 Small RNAs are short non-coding RNAs with an average length of 18-30 nt, including miRNA, siRNA and piRNA, which play important roles in regulatory processes. The BMKCloud sRNA pipeline provides both standard and custom analysis for miRNA identification. After read trimming and quality control, reads are aligned against multiple databases to classify sRNAs and select miRNAs and mapped to the reference genome. miRNAs are identified based on known miRNA databases, providing information on secondary structure, miRNA family and target genes. Differential expression analysis identifies differentially expressed miRNAs and the corresponding target genes are functionally annotated to find enriched categories.