Fa'asologa o Vaega Fa'aopoopo Fa'apitoa-Locus (SLAF-Seq)
Au'aunaga Fa'amatalaga
Fuafuaga Fa'atekinisi
Fa'agasologa o galuega
Tulaga Auaunaga
Maua maualuga le mauaina o maka- O tekonolosi fa'asologa maualuga e fesoasoani i le SLAF-Seq i le mauaina o le fiaselau afe o pine i totonu o le genome atoa.
Fa'alagolago maualalo ile genome- E mafai ona faʻaoga i ituaiga a le o iai pe leai foi se genome faʻasino.
Fuafuaga fuafuaga fetuutuunai- Tasi-enzyme, lua-enzyme, tele-enzyme digestion ma ituaiga eseese o enzymes, e mafai ona filifilia uma e faʻafetaui sini suʻesuʻega eseese poʻo ituaiga.E fa'aoga muamua le su'esu'ega ile silico e fa'amautinoa ai se mamanu ole enzyme sili.
Fa'amama fa'ama'i lelei- Sa faia muamua le fa'ata'ita'iga e fa'amalieina tulaga, lea e fa'amautu ai le fa'ata'ita'iga aloa'ia ma fa'atuatuaina.E mafai ona ausia le sili atu i le 95% le lelei o le aoina o vaega.
Fa'asoa tutusa fa'ailoga SLAF- O fa'ailoga SLAF o lo'o tufatufaina tutusa i chromosomes uma i le tele, ausia le averesi o le 1 SLAF i le 4 kb.
Aloese lelei mai le toe faia- Faʻasologa faʻasolosolo i faʻamaumauga SLAF-Seq e faʻaititia i lalo ifo o le 5%, aemaise lava i ituaiga o loʻo i ai le maualuga o le toe fai, e pei o saito, saga, ma isi.
Tele poto masani- I luga o le 2000 tapunia SLAF-Seq poloketi i luga o le faitau selau o ituaiga e aofia ai laau, mamame, manulele, iniseti, vai-organisms, ma isi.
Fa'atupuina e le tagata lava ia bioinformatic workflow- O se su'esu'ega fa'akomepiuta tu'ufa'atasia mo le SLAF-Seq na fausia e le BMKGENE e fa'amautinoa ai le fa'amaoni ma le sa'o o galuega fa'ai'u.
Fa'amatalaga Auaunaga
| Fa'avae | Conc.(ng/gl) | Aofa'iga (ug) | OD260/280 |
| Illumina NovaSeq | >35 | >1.6(Volun>15μl) | 1.6-2.5 |
Fuafuaga Fautuaina Fa'asologa
Fa'asologa loloto: 10X/Tag
| Genome Tele | Fautuaina SLAF Tag |
| <500 Mb | 100K poʻo le WGS |
| 500 Mb- 1 Gb | 100 K |
| 1 Gb -2 Gb | 200 K |
| Genomes tetele pe lavelave | 300 - 400K |
| Talosaga
| Fautuaina Fua o Tagata
| Fa'asologa o fuafuaga ma le loloto
| |
| loloto
| Numera Fa'ailoga
| ||
| GWAS
| Fa'ata'ita'iga numera ≥ 200
| 10X
|
Ae faimai foi tele genome
|
| Genetic Evolution
| Tagata ta'ito'atasi vaega laiti ≥ 10; atoa fa'ata'ita'iga ≥ 30
| 10X
| |
Fautuaina Fa'ata'ita'iga Tu'uina atu
Pusa: 2 ml centrifuge paipa
Mo le tele o faʻataʻitaʻiga, matou te fautuaina e aua le faʻasaoina i le ethanol.
Fa'ailoga fa'ata'ita'iga: E mana'omia le fa'ailoga manino o fa'ata'ita'iga ma ia tutusa ma fa'amatalaga fa'ata'ita'iga ua tu'uina atu.
La'uina: Fa'amamago-aisa: Fa'ata'ita'iga e mana'omia ona teu muamua i taga ma tanu i le aisa-mago.
Au'aunaga Galuega
Fa'ata'ita'iga QC
Fa'ata'ita'iga pailate
SLAF-faʻataʻitaʻiga
Sauniuniga Faletusi
Fa'asologa
Fa'amatalaga Fa'amatalaga
Au'aunaga ina ua uma ona fa'atau
1. Fuainumera o taunuuga o faafanua
2. Atina'e fa'ailoga SLAF
3. Fa'amatalaga eseese
| Tausaga | Tusitala | IF | Ulutala | Talosaga |
| 2022 | Fesootaiga faanatura | 17.694 | Genomic faavae o giga-chromosomes ma giga-genome o laau peony Paeonia ostii | SLAF-GWAS |
| 2015 | Phytologist Fou | 7.433 | O tulagavae o fale e taula ai itulagi genomic e taua agronomic i soya | SLAF-GWAS |
| 2022 | Tusitala o Su'esu'ega Maualuga | 12.822 | Genome-lautele fa'aoso introgressions o Gossypium barbadense i G. hirsutum fa'aalia loci sili mo le fa'aleleia fa'atasi o le lelei o alava filo ma fua mai uiga | SLAF-Evolutionary genetics |
| 2019 | La'au mole | 10.81 | Population Genomic Analysis ma De Novo Assembly Fa'aalia le Afuaga o Weedy Alaisa ose Taaloga Evolutionary | SLAF-Evolutionary genetics |
| 2019 | Natura Genetics | 31.616 | Genome sequence ma kenera eseese o le carp masani, Cyprinus carpio | SLAF-So'oga fa'afanua |
| 2014 | Natura Genetics | 25.455 | Ole genome ole pinati fa'ato'aina e maua ai le fa'amatalaga ile karyotypes legume, polyploid evolusione ma fa'ato'aga fa'ato'aga. | SLAF-So'oga fa'afanua |
| 2022 | La'au Biotechnology Journal | 9.803 | O le fa'ailoaina ole ST1 o lo'o fa'ailoa mai ai se filifiliga e a'afia ai le so'oga o fatu fatu ma le suau'u o lo'o iai i le taimi o le fa'atauina o soya | SLAF-Fa'ailoga atina'e |
| 2022 | Tusitala Faava o Malo o Saienisi Molecular | 6.208 | Fa'ailoaina ma le Atina'eina o Fa'ailoga DNA mo se Wheat-Leymus mollis 2Ns (2D) Suiga Disomic Chromosome | SLAF-Fa'ailoga atina'e |
