Fa'aitiitiga Fa'atusa o le Bisulfite Sequencing (RRBS)
Fa'amatalaga Auaunaga
Tulaga: NovaSeq tulaga, PE150
Ituaiga faletusi: 200-400bp fa'aofi le faletusi DNA fa'aogaina le bisulfite
Fuafuaga fa'asologa: Fa'atasi-Fa'ai'uga 150 bp
Fautuaina fa'amatalaga fa'amatalaga: 10 Gb raw data/fa'ata'ita'iga
Fa'ata'ita'iga Manaoga
DNA aofaʻi: ≥ 2ug
DNA fa'amauina: ≥ 20ng/μl.
Mama: OD260/280 = 1.8 i le 2.0 e aunoa ma le faʻaleagaina poʻo le RNA faʻaleagaina
Bioinformatics su'esu'ega
Auala o Galuega Au'aunaga
Fa'ata'ita'iga tu'uina atu
Fauga faletusi
Fa'asologa
Iloiloga fa'amaumauga
Au'aunaga pe a uma ona fa'atau
a.Fa'asinoga fa'asologa o fa'amaumauga o le genome
Fa'amaumauga o fa'afanua faitau
Sfuainumera o le loloto o le fa'asologa ma le fa'asalalauina o le nofoaga C
Afa'aputuina fa'asalalauga
Dfa'atosina lelei o fa'ata'ita'iga ta'itasi
b.Su'eina nofoaga o le methylation
Lisi o maualuga o le methylation ile C site
BS liua
Methylated cytosine
Tufatufaina tulaga o le methylation
c.Methylation faafanua
Genome-lautele
Gelemene elemene
d.Fa'atusatusaga o tulaga methylation i le va o fa'ata'ita'iga
PAirwise Fa'atasiga ole CG methylation
PCA-CG methylation
Clustering o le CG methylation
d.Su'esu'ega methylation eseese
Sfuainumera o DMR
DMR tufatufaina umi
H'ai faafanua
DTufatufaina o le MR ile itulagi fa'atatau
