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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
O le ChIP-Seq e tu'uina atu ai le fa'avasegaina o le genome-lautele o taula'iga DNA mo suiga fa'asolopito, fa'asologa o tusitusiga, ma isi polotini e feso'ota'i DNA.E tu'ufa'atasia le filifilia o le chromatin immuno-precipitation (ChIP) e toe fa'aleleia ai fa'alavelave fa'apolotini-DNA fa'apitoa, fa'atasi ai ma le mana o le fa'asologa o le isi augatupulaga (NGS) mo le fa'asologa maualuga o le DNA toe maua.E le gata i lea, talu ai o le protein-DNA lavelave e toe maua mai sela ola, e mafai ona faʻatusatusa nofoaga o loʻo fusifusia i ituaiga sela eseese ma aano, pe i lalo o tulaga eseese.O talosaga e amata mai i tulafono faatonutonu tusitusia i auala tau atina'e i faiga fa'ama'i ma tua atu.
Tulaga: Illumina NovaSeq Platform
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Metagenomic Sequencing -NGS
Metagenome e fa'atatau i le aofa'i o mea fa'atupu o se fa'alapotopotoga fefiloi o meaola, e pei o le si'osi'omaga metagenome, tagata metagenome, ma isi. O lo'o iai genomes o meaola ninii e mafai ona fa'ato'aina ma mea e le mafai ona fa'ato'aina.Metagenomic sequencing ose meafaigaluega mole faʻaaogaina e suʻesuʻe ai mea faʻafefiloi genomic na maua mai faʻataʻitaʻiga o le siosiomaga, lea e maua ai faʻamatalaga auiliili i ituaiga eseese ma le tele, fausaga o le faitau aofaʻi, sootaga phylogenetic, genes galue ma fesoʻotaʻiga fesoʻotaʻiga ma mea tau le siosiomaga.
Fa'avae:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics o se meafaigaluega mole faʻaaogaina e suʻesuʻe ai mea faʻafefiloi genomic na maua mai faʻataʻitaʻiga o le siosiomaga, lea e maua ai faʻamatalaga auiliili i ituaiga eseese ma le tele, fausaga o le faitau aofaʻi, sootaga phylogenetic, genes galue ma fesoʻotaʻiga fesoʻotaʻiga ma mea tau siosiomaga, ma isi. i suʻesuʻega metagenomic.O lona fa'atinoga mata'ina i le umi faitau na fa'asilisiliina i lalo ole vaitafe metagenomic su'esu'ega, aemaise metagenome fa'apotopotoga.O le fa'aogaina o le faitau-umi, su'esu'ega metagenomic fa'avae Nanopore e mafai ona ausia atili fa'apotopotoga fa'aauau pe a fa'atusatusa i metagenomics fana fana.Ua faʻasalalauina o le Nanopore-based metagenomics na faʻataunuʻuina manuia ma faʻamaeʻaina siama siama mai microbiomes (Moss, EL, et. al,Natura Biotech, 2020)
Fa'avae:Nanopore PromethION P48
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Fa'asologa atoa o le genome bisulfite
DNA methylation i le tulaga lona lima i le cytosine (5-mC) o loʻo i ai se aʻafiaga taua i le faʻaalia o le kenera ma gaioiga feaveaʻi.O faʻasologa o le methylation e le masani ai ua fesoʻotaʻi ma le tele o tulaga ma faʻamaʻi, e pei o le kanesa.O le WGBS ua avea ma tulaga auro mo le suʻesuʻeina o le genome-wide methylation ile faʻavae tasi.
Tulaga: Illumina NovaSeq Platform
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Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)
ATAC-seq o se metotia faʻasolosolo maualuga mo le suʻesuʻeina o le genome-wide chromatin accessibility, lea e taua mo le pulea epigenetic lalolagi o faʻamatalaga o kenera.E fa'apipi'i fa'asologa fa'asologa i vaega o chromatin matala e ala ile Tn5 transposase.A maeʻa le PCR faʻateleina, e fausia se faletusi faʻasologa.O vaega uma o le chromatin e mafai ona maua i lalo o se tulaga avanoa-taimi fa'apitoa, e le gata i nofoaga o lo'o fusifusia ai se vaega fa'aliliuga, po'o se vaega fa'asologa fa'asolosolo.
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16S/18S/ITS Amplicon Sequencing-PacBio
O le vaega pito i luga ole 16S ma le 18S rRNA o lo'o i ai vaega e lua e sili ona fa'asao ma hyper-feuia'i itulagi ose mole mole tamatamai lima mole prokaryotic ma eukaryotic organisms faailoagofieina.I le faʻaaogaina o le faʻasologa, o nei amplicons e mafai ona faʻatatau i luga o vaega faʻasao ma o le hyper-variable regions e mafai ona faʻamalamalamaina atoatoa mo microbial identification e fesoasoani i suʻesuʻega e aofia ai microbial diversity analysis, taxonomy, phylogeny, ma isi. ) fa'asologa o PacBio platform e mafai ai ona maua le sa'o sa'o umi o faitau, lea e mafai ona aofia ai amplicons atoa (pe. 1.5 Kb).O le fa'alauteleina o le va'aiga o fa'atupu kenera na matua fa'aleleia atili ai le fa'ai'uga i fa'amatalaga o ituaiga i siama po'o sigi nu'u.
Fa'avae:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing e fa'amoemoe e fa'aalia phylogeny, taxonomy, ma le tele o ituaiga o meaola i totonu o le microbial community e ala i le su'esu'eina o oloa PCR o fa'ailoga fa'ailoga o le fale o lo'o aofia uma ai vaega e sili ona felafolafoa'i ma hypervariable.O le fa'aofiina o nei fa'ata'ita'iga mole mole lelei atoatoa e Woeses et al, (1977) fa'amalosia ai le fa'avasegaina o microbiome e leai se tu'ufua.O le fa'asologa o le 16S (bacteria), 18S (fungi) ma le Internal transcribed spacer(ITS, fungi) e mafai ai ona fa'ailoaina le tele o ituaiga fa'apea fo'i ma ituaiga e seasea fa'ailoaina.O lenei tekinolosi ua avea ma mea faigaluega lautele ma tele i le faailoaina o eseesega microbial tuufaatasiga i siosiomaga eseese, e pei o le gutu o le tagata, inumaga, feces, ma isi.
Fa'avae:Illumina NovaSeq Platform
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Fa'asologa o Genome Atoa Fa'ama'i ma Fungal
O le toe fa'asologa o genome atoa o siama ma fungal ose meafaigaluega taua e fa'amae'a ai genomes o siama ma sigi ua iloa, fa'apea fo'i ma le fa'atusatusaina o le tele o genomes po'o le fa'afanua genomes o meaola fou.E taua tele le fa'asologa o genomes atoa o siama ma sigi ina ia mafai ona maua ai genomes fa'asino sa'o, e fai ai fa'amatalaga microbial ma isi su'esu'ega fa'atusa o le genome.
Tulaga: Illumina NovaSeq Platform
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PacBio-Umi atoa 16S/18S/ITS Amplicon Sequencing
Amplicon (16S / 18S / ITS) faʻavae ua atiaʻe ma tausaga o le poto masani i suʻesuʻega faʻavae microbial diversity, lea e aofia ai suʻesuʻega faʻapitoa ma auʻiliʻiliga faʻapitoa: auʻiliʻiliga faʻavae e aofia ai le auʻiliʻiliga autu o suʻesuʻega microbial o loʻo i ai nei, o le auʻiliʻiliga o mea e tamaoaiga ma atoatoa, ma fa'ai'uga au'ili'ili o lo'o tu'uina atu ile tulaga o lipoti o poloketi;O le anotusi o au'ili'iliga fa'apitoa e 'ese'ese.E mafai ona filifilia faʻataʻitaʻiga ma faʻamaufaʻailoga e mafai ona faʻatulagaina fetuutuunai e tusa ai ma le lipoti o suʻesuʻega autu ma le faʻamoemoega o suʻesuʻega, e iloa ai manaʻoga faʻapitoa.Windows operating system, faigofie ma vave.
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PacBio-Full-length Transcriptome (Leai Fa'asinoga)
Avea fa'amaumauga ole fa'asologa o le Pacific Biosciences (PacBio) Isoform e fai ma fa'aoga, e mafai e lenei App ona fa'ailoa fa'asologa fa'asologa atoa (e aunoa ma se fa'apotopotoga).E ala i le fa'avasegaina o fa'asologa atoa e fa'atatau i le genome fa'asinomaga, e mafai ona fa'amaninoina fa'amaumauga e genes ua iloa, transcripts, coding regions, ma isi. I lenei tulaga, e sili atu le fa'amaoniaina o fausaga mRNA, e pei o isi splicing, ma isi, e mafai ona ausia.O su'esu'ega fa'atasi ma fa'amaumauga fa'asologa fa'asologa o le NGS e mafai ai ona sili atu fa'amatalaga fa'amatalaga ma sili atu le sa'o fa'atatau i fa'amatalaga i le tulaga fa'amaumauga, lea e tele fa'amanuiaga fa'aaliga fa'a-va'e ma su'esu'ega fa'atino.
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Fa'aitiitiga Fa'atusa o le Bisulfite Sequencing (RRBS)
Ole su'esu'ega ole DNA methylation e masani lava ona avea ma autu vevela ile su'esu'ega fa'ama'i, ma e feso'ota'i vavalalata ma fa'amatalaga o kenera ma uiga fa'apitoa.RRBS o se auala saʻo, lelei ma taugofie mo suʻesuʻega DNA methylation.Fa'atamaoaigaina o le fa'alauiloaina ma le CpG motu motu e ala i le enzymatic cleavage (Msp I), fa'atasi ma le fa'asologa o le Bisulfite, e maua ai le su'esu'ega o le DNA methylation maualuga.
Tulaga: Illumina NovaSeq Platform
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Prokaryotic mRNA fa'asologa
MRNA sequencing e faʻamalosia ai le faʻamalamalamaina atoatoa o tusitusiga mRNA uma i totonu o sela i lalo o tulaga faʻapitoa.O lenei tekonolosi fa'aonaponei e avea o se meafaigaluega malosi, fa'aalia fa'amatalaga lavelave fa'aaliga o kenera, fausaga o kenera, ma masini mole mole e feso'ota'i ma faiga fa'aola eseese.Fa'aaoga lautele i su'esu'ega fa'avae, su'esu'ega fa'afoma'i, ma le atina'eina o vaila'au, fa'asologa o le mRNA e maua ai fa'amatalaga i le lavelave o le fa'aogaina o le telefoni feavea'i ma le fa'atonutonuina o kenera.O la matou fa'ata'ita'iga fa'ata'ita'iga o le mRNA prokaryotic ua fa'atulagaina mo transcriptomes prokaryotic, e aofia ai le fa'aitiitiga o le rRNA ma le sauniaina o le faletusi.
Fa'avae: Illumina NovaSeq X
