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Metagenomic Sequencing -NGS
Metagenome e fa'atatau i le aofa'i o mea fa'atupu o se fa'alapotopotoga fefiloi o meaola, e pei o le si'osi'omaga metagenome, tagata metagenome, ma isi. O lo'o iai genomes o meaola ninii e mafai ona fa'ato'aina ma mea e le mafai ona fa'ato'aina.Metagenomic sequencing ose meafaigaluega mole faʻaaogaina e suʻesuʻe ai mea faʻafefiloi genomic na maua mai faʻataʻitaʻiga o le siosiomaga, lea e maua ai faʻamatalaga auiliili i ituaiga eseese ma le tele, fausaga o le faitau aofaʻi, sootaga phylogenetic, genes galue ma fesoʻotaʻiga fesoʻotaʻiga ma mea tau le siosiomaga.
Fa'avae:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics o se meafaigaluega mole faʻaaogaina e suʻesuʻe ai mea faʻafefiloi genomic na maua mai faʻataʻitaʻiga o le siosiomaga, lea e maua ai faʻamatalaga auiliili i ituaiga eseese ma le tele, fausaga o le faitau aofaʻi, sootaga phylogenetic, genes galue ma fesoʻotaʻiga fesoʻotaʻiga ma mea tau siosiomaga, ma isi. i suʻesuʻega metagenomic.O lona fa'atinoga mata'ina i le umi faitau na fa'asilisiliina i lalo ole vaitafe metagenomic su'esu'ega, aemaise metagenome fa'apotopotoga.O le fa'aogaina o le faitau-umi, su'esu'ega metagenomic fa'avae Nanopore e mafai ona ausia atili fa'apotopotoga fa'aauau pe a fa'atusatusa i metagenomics fana fana.Ua faʻasalalauina o le Nanopore-based metagenomics na faʻataunuʻuina manuia ma faʻamaeʻaina siama siama mai microbiomes (Moss, EL, et. al,Natura Biotech, 2020)
Fa'avae:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
O le vaega pito i luga ole 16S ma le 18S rRNA o lo'o i ai vaega e lua e sili ona fa'asao ma hyper-feuia'i itulagi ose mole mole tamatamai lima mole prokaryotic ma eukaryotic organisms faailoagofieina.I le faʻaaogaina o le faʻasologa, o nei amplicons e mafai ona faʻatatau i luga o vaega faʻasao ma o le hyper-variable regions e mafai ona faʻamalamalamaina atoatoa mo microbial identification e fesoasoani i suʻesuʻega e aofia ai microbial diversity analysis, taxonomy, phylogeny, ma isi. ) fa'asologa o PacBio platform e mafai ai ona maua le sa'o sa'o umi o faitau, lea e mafai ona aofia ai amplicons atoa (pe. 1.5 Kb).O le fa'alauteleina o le va'aiga o fa'atupu kenera na matua fa'aleleia atili ai le fa'ai'uga i fa'amatalaga o ituaiga i siama po'o sigi nu'u.
Fa'avae:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing e fa'amoemoe e fa'aalia phylogeny, taxonomy, ma le tele o ituaiga o meaola i totonu o le microbial community e ala i le su'esu'eina o oloa PCR o fa'ailoga fa'ailoga o le fale o lo'o aofia uma ai vaega e sili ona felafolafoa'i ma hypervariable.O le fa'aofiina o nei fa'ata'ita'iga mole mole lelei atoatoa e Woeses et al, (1977) fa'amalosia ai le fa'avasegaina o microbiome e leai se tu'ufua.O le fa'asologa o le 16S (bacteria), 18S (fungi) ma le Internal transcribed spacer(ITS, fungi) e mafai ai ona fa'ailoaina le tele o ituaiga fa'apea fo'i ma ituaiga e seasea fa'ailoaina.O lenei tekinolosi ua avea ma mea faigaluega lautele ma tele i le faailoaina o eseesega microbial tuufaatasiga i siosiomaga eseese, e pei o le gutu o le tagata, inumaga, feces, ma isi.
Fa'avae:Illumina NovaSeq Platform
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Fa'asologa o Genome Atoa Fa'ama'i ma Fungal
O le toe fa'asologa o genome atoa o siama ma fungal ose meafaigaluega taua e fa'amae'a ai genomes o siama ma sigi ua iloa, fa'apea fo'i ma le fa'atusatusaina o le tele o genomes po'o le fa'afanua genomes o meaola fou.E taua tele le fa'asologa o genomes atoa o siama ma sigi ina ia mafai ona maua ai genomes fa'asino sa'o, e fai ai fa'amatalaga microbial ma isi su'esu'ega fa'atusa o le genome.
Tulaga: Illumina NovaSeq Platform
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Metatranscriptome Sequencing
Metatranscriptome sequencing fa'ailoaina le fa'aaliga o kenera o microbes (eukaryotes uma ma prokaryotes) i totonu o si'osi'omaga fa'anatura (e pei o le palapala, vai, sami, feces, ma gut.) Aemaise lava, o nei 'au'aunaga e mafai ai ona e maua atoa fa'amatalaga fa'amatalaga fa'amatalaga o nu'u lavelave microbial, iloiloga taxonomic o ituaiga, su'esu'ega fa'atamaoaigaina o ga'o eseese fa'aalia, ma isi mea.
Tulaga: Illumina NovaSeq Platform
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Fungal Genome
Biomarker Technologies e tuʻuina atu suʻesuʻega o le genome, genome lelei ma faʻamaeʻaina genome o gaʻo e faʻatatau ile sini suʻesuʻe.Genome sequencing, faʻapotopotoga ma faʻamatalaga aoga e mafai ona ausia e ala i le tuʻufaʻatasia o le Generation sequencing + Faʻasologa o augatupulaga lona tolu e ausia ai le maualuga o le genome assembly.E mafai fo'i ona fa'aogaina le tekonolosi Hi-C e fa'afaigofie ai le fa'apotopotoina o le genome ile tulaga ole chromosome.
Fa'avae:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Bacteria Complete Genome
Biomarker Technologies e tu'uina atu auaunaga fa'asologa i le fausiaina o genome atoatoa o siama ma leai se avanoa.O galuega autu o siama faʻamaeʻaina fau genome e aofia ai le faʻasologa o augatupulaga lona tolu, faʻapipiʻi, faʻamatalaga galuega ma suʻesuʻega bioinformatic alualu i luma faʻataunuʻuina sini maoti suʻesuʻega.O se fa'amatalaga sili atu ona manino o le genome siama e fa'amalosia ai le fa'aaliaina o faiga fa'avae o lo'o fa'avaeina a latou gaioiga fa'ale-natura, lea e mafai fo'i ona maua ai fa'amatalaga taua mo su'esu'ega genomic i ituaiga eukaryotic maualuga.
Fa'avae:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
