Avea fa'amaumauga ole fa'asologa o le Pacific Biosciences (PacBio) Isoform e fai ma fa'aoga, e mafai e lenei App ona fa'ailoa fa'asologa fa'asologa atoa (e aunoa ma se fa'apotopotoga).E ala i le fa'avasegaina o fa'asologa atoa e fa'atatau i le genome fa'asinomaga, e mafai ona fa'amaninoina fa'amaumauga e genes ua iloa, transcripts, coding regions, ma isi. I lenei tulaga, e sili atu le fa'amaoniaina o fausaga mRNA, e pei o isi splicing, ma isi, e mafai ona ausia.O su'esu'ega fa'atasi ma fa'amaumauga fa'asologa fa'asologa o le NGS e mafai ai ona sili atu fa'amatalaga fa'amatalaga ma sili atu le sa'o fa'atatau i fa'amatalaga i le tulaga fa'amaumauga, lea e tele fa'amanuiaga fa'aaliga fa'a-va'e ma su'esu'ega fa'atino.