GENOME EVOLUTION
Genome De novo
Fonotaga|Fa'ai'uga fa'afeusuaiga O galuega fa'asologa atoa ma 'au'aunaga o le bioinformatic vaega na tu'uina atu e Biomarker Techonologies.
Pu'upu'u
“O le fa'ailoga iloga o seadragons e aofia ai mea fa'apipi'i pei o lau, o le gutu tubular e leai ni nifo, ma le ma'itaga o tama e aofia ai le fa'atupuina o fuamoa fa'afelalelei i luga o se "fa'ato'aga" matala.Matou te faia fa'asologa fa'asologa o tane ma fafine o le tarako masani (Phyllopteryx taeniolatus) ma ona ituaiga vavalalata vavalalata, o le alligator pipefish (Syngnathoides biaculeatus).Fa'amatalaga fa'aliliuga mai se mea fou fa'afuafua, o fa'aopoopoga e pei o laula'au, o lo'o fa'aalia ai o se seti o kenera e masani ona a'afia i le atina'eina o apa'u ua fa'atasia fa'apea fo'i ma le fa'atamaoaigaina o tusitusiga fa'aliliu mo le toe fa'aleleia o ga'o ma kenera puipuia puipuia.O le zebrafish mutants mo scpp5, lea e leiloa i syngnathids uma, na maua e leai se mea poʻo ua faʻaleagaina nifo o le pharyngeal, e lagolagoina ai le manatu o le leiloa o le scpp5 ua mafua ai le gau o nifo i le syngnathids.O se nofoaga e fa'atatau i itupa e fa'avasegaina ai se kenera amhr2y fa'apitoa tama e fa'asoa e seadragon masani ma i'a paipa alligator na iloa. ”
Mea ma metotia:
Meafaitino
Fla tatou tarako masani (P. taeniolatus) ma i'a paipa alligator e lua (S. biaculeatus) fa'ata'ita'iga.E lua seadragon masani (tasi le tane ma le tasi fafine) ma le lua alligator pipefish (tasi le tane ma le tasi fafine) tagata na faʻaogaina mo le faʻasologa atoa-genome, ae lua isi tane masani seadragon na faʻaogaina mo le genome resequencing.
Genome Sequencing fuafuaga
270 / 350 bp faletusi (Hiseq 2500) + Pacbio 20Kb (Sequel) poʻo Nanopore 30 Kb (MinION) + Hi-C (NovaSeq 6000) .faʻasologa, ma isi tane e toʻalua seadragon masani na faʻaaogaina mo le genome resequencing.
Transcriptome Sequencing
Fpo'o se tarako masani, fai'ai, mata, gill, ate, fatu, fatuga'o, mesentery, inumaga, musele, apa, pa'u, mea e pei o laulaau, su'esu'e, ma ovary sa aoina.
Genome de novo fa'apotopotoga ta'iala
Canu (Fa'asa'oga) + WTDBG2 (Fa'apotopotoga) + Pilon(Polani) + Lachesis (Hi-C).
I'uga Autu
Ata 1. O vaega taua o le tarako masani (P. taeniolatus) ma le i'a paipa alligator (S. biaculeatus) ma o latou tulaga phylogenetic.
Ata 2. Fa'avasegaga ma le fa'atupuina fa'atupu o le fa'aopoopoga pei o lau i le tarako masani (P. taeniolatus).
Ata 3. Gane fa'ata'ita'i fa'aigoa i le tarako masani (P. taeniolatus) ma le i'a paipa alligator (S. biaculeatus).
Ata 4. Fa'ailoga fa'aigoa o nifo i le zebrafish scpp5 mutants homozygous.
Taimi meli: Ian-08-2022