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条形 fu'a-03

Oloa

Metabolomics

O le metabolome o le oloa pito i lalo o le genome ma e aofia ai le aofaʻi atoa o molelaʻa maualalo-mole-mamafa (metabolites) i totonu o se sela, aano, poʻo le tino.Metabolomics faʻamoemoe e fua le lautele lautele o molelaʻau laiti i le tulaga o faʻamaʻi faʻaletino poʻo faʻamaʻi.Metabolomics methodologis e pa'ū i ni vaega ma'oti se lua: metabolomics e le'i fa'atatauina, o se su'esu'ega atoatoa fa'atatau o su'esu'ega fa'atatau uma i totonu o se fa'ata'ita'iga e aofia ai vaila'au e le o iloa e fa'aaoga ai le GC-MS/LC-MS, ma metabolomics fa'atatauina, o le fuaina o vaega fa'amatalaina o vaila'au fa'apitoa ma metabolites fa'amatalaga biochemically.


Au'aunaga Fa'amatalaga

Fa'ata'ita'iga Manaoga

Metabolomics e le fa'atatauinaFa'ata'ita'iga ituaiga

Fa'ata'ita'iga fautuainaaofaiga

Fa'asologa o mea ola

LC-MS/GC-MSmetabolomics e le'i fa'atatauina

Tisi

200mg

La'au ≥6;

Manu ≥10;

Tagata ≥30;

Plasma/serum

200ul

Fecal/Intestinal Contents

150mg

sela

1*106

mimi

500ul

Suavai Rumen

1ml

Metabolomics taulaiFa'ata'ita'iga ituaiga

Fautuaina le aofaʻi o faʻataʻitaʻiga

Fa'asologa o mea ola

Phytohormone/Tryptophane/Malosi'aga Metabolism/ Lipid fa'asa'o/ Carotenoid;karotinoid

Tisi

500mg

La'au ≥3;

Manu ≥6;

Plasma/serum

500ul

Fecal

1000mg

sela

1*107

Fa'amatalaga Auaunaga

Metabolomics e le fa'atatauina:
1. Tekinolosi matua ma mautu, iugafono maualuga ma filifili lelei
2. Fa'atumu fa'amaumauga;
3. E talafeagai mo le suʻesuʻeina o matrices lavelave, tuʻuina atu faʻamatalaga auiliili i se tasi auiliiliga;

Metabolomics fa'atatau:
1. Fa'aaoga le fa'amaufa'ailoga fa'alotoifale fa'amaonia e fa'aleleia atili ai le sa'o lelei ma le fa'atusatusaina o vaila'au;
2.Quality control system e faʻamautinoa ai le faʻamaoni o faʻamaumauga.

Auala o Galuega Au'aunaga

1. Non-targeted metabolomics

11

2. Metabolomics fa'atatau

22

3.Widely taulaʻi metabolomics

33

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