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Epigenetics

  • Chromatin Immunoprecipitation Sequencing (ChIP-seq)

    Chromatin Immunoprecipitation Sequencing (ChIP-seq)

    O le ChIP-Seq e tu'uina atu ai le fa'avasegaina o le genome-lautele o sini DNA mo le suiga o fa'ailoga, fa'asologa o tusitusiga, ma isi polotini e feso'ota'i DNA.E tu'ufa'atasia le filifilia ole chromatin immuno-precipitation (ChIP) e toe fa'aleleia ai fa'alavelave fa'apolotini-DNA fa'apitoa, fa'atasi ai ma le mana o le fa'asologa o le isi augatupulaga (NGS) mo le fa'asologa maualuga o le DNA toe maua.E le gata i lea, talu ai o le protein-DNA lavelave e toe maua mai sela ola, e mafai ona faʻatusatusa nofoaga o loʻo fusifusia i ituaiga sela eseese ma aano, pe i lalo o tulaga eseese.Talosaga e afua mai tulafono fa'aliliu tusi i ala atina'e i faiga fa'ama'i ma tua atu.

    Fa'avae: Illumina NovaSeq 6000

  • Whole genome bisulfite sequencing

    Fa'asologa atoa o le genome bisulfite

    DNA methylation i le tulaga lona lima i le cytosine (5-mC) o loʻo i ai se aʻafiaga faʻavae i luga o faʻamatalaga o kenera ma gaioiga feaveaʻi.O faʻasologa o le methylation e le masani ai ua fesoʻotaʻi ma le tele o tulaga ma faʻamaʻi, e pei o le kanesa.Ua avea le WGBS ma tulaga auro mo le suʻesuʻeina o le genome-wide methylation ile faʻavae tasi.

    Fa'avae: Illumina NovaSeq6000

  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

    Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)

    ATAC-seq o se metotia faʻasologa maualuga mo le suʻesuʻeina o le genome-wide chromatin accessibility, lea e taua mo le pulea epigenetic lalolagi o faʻamatalaga o kenera.O fa'asologa fa'asologa o lo'o fa'aofiina i totonu o le chromatin eria e le hyperactive Tn5 transposase.A maeʻa le PCR faʻalauteleina, e fausia se faletusi faʻasologa.O vaega uma o le chromatin e mafai ona maua i lalo o se tulaga avanoa-taimi patino, e le gata i le faʻatapulaʻaina o nofoaga o le faʻasologa o tusitusiga, poʻo se vaega faʻapitoa ua suia.

  • Reduced Representation Bisulfite Sequencing (RRBS)

    Fa'aitiitiga Fa'atusa o le Bisulfite Sequencing (RRBS)

    Ole su'esu'ega ole DNA methylation e masani lava ona avea ma autu vevela ile su'esu'ega fa'ama'i, ma e feso'ota'i vavalalata ma fa'amatalaga o kenera ma uiga fa'apitoa.RRBS o se auala saʻo, lelei ma taugofie mo suʻesuʻega DNA methylation.Fa'atamaoaigaina o le fa'alauiloaina ma le CpG motu motu e ala i le enzymatic cleavage (Msp I), fa'atasi ma le fa'asologa o le Bisulfite, e maua ai le su'esu'ega o le DNA methylation maualuga.

    Fa'avae: Illumina NovaSeq 6000

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