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条形 fu'a-03

Oloa

DNA/RNA Fa'asologa - Nanopore Sequencer

O le fa'asologa o le ONT o se tasi mole mole taimi moni fa'atekonolosi fa'asologa o fa'ailoga eletise e fa'avae i luga o nanopores, o le fa'asologa fa'asologa o fa'avae ta'itasi e tutusa.DNA / RNA fa'alua fa'alua o le a fusifusia i porotini nanoporous fa'apipi'i i totonu o le biofilm ma fa'amuta i lalo o le ta'ita'iga o le porotini afi, i lalo o le gaioiga o le eseesega o le voltage mai itu uma e lua o le biofilm, o le DNA/RNA e ui atu i le nanopore channel protein i se tulaga patino. fua faatatau.Ona o le eseesega o mea tau vailaʻau o faʻavae eseese i luga o le DNA / RNA strand, pe a oʻo atu se faʻavae se tasi poʻo le DNA mole mole i totonu o le nanopore channel, o le a mafua ai le suiga o faailo eletise eseese.E ala i le suʻesuʻeina ma fetaui ma nei faʻailoga, e mafai ona faʻatusatusa ituaiga faʻavae tutusa, ma e mafai ona faʻamaeʻaina le suʻesuʻeina o le taimi moni o le faasologa.


Au'aunaga Fa'amatalaga

Fa'aaliga Fa'aaliga

Au'aunaga Fa'amatalaga Fa'amatalaga

Fa'avae

Tele o Faletusi

Fa'amatalaga Fa'amatalaga Fa'amatalaga (Per Sel)

Fa'asa'o Tutoatasi

Talosaga

Nanopore

8Kb, 10kb, 20kb, Ultralong, cDNA-PCR

70-90Gb/Sele

85-92%

SV vala'au, De novo, Fa'asologa atoa, Iso-Seq, Gene fa'amatalaga, Su'esu'eina ole DNA methylation

Tulaga Auaunaga

● I luga o le 5 tausaga le poto masani i luga o le PacBio sequencing platform ma le faitau afe o galuega tapunia ma ituaiga eseese.
● BMKGENE ose paaga aloaʻia a Oxford Nanopore, faʻatasi ai ma faʻailoga lua RNA/DNA.
● O loʻo iai faʻataʻitaʻiga masani o faʻasologa faʻatasi ma meafaigaluega atoatoa ma lava faʻasologa faʻasologa.
● Faʻavae i luga o le Nanopore platform, e sili atu i le 10 manu ma laʻau suʻesuʻega Denovo ua lomia i tusitala faʻavaomalo lauiloa.

Fa'ata'ita'iga Manaoga


Ituaiga Fa'ata'ita'i

Aofaiga

Fa'atonuga(Qubit ®)

Volume

Mama

O isi

Genomic DNA

Fa'alagolago i Fa'amatalaga Manaomia

 ≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

Malamalama pito i luga ile 260 nm, leai se faʻaleagaina

E mana'omia le fuaina o le fa'atonuga e Qubit ma Qubit/Nanopore ≤ 2

RNA atoa

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5;leai se fa'aleagaina

RIN tau ≥7.5

 

Au'aunaga Galuega

sauniuniga faataitai

Fa'ata'ita'iga sauniuniga

Sauniuniga Faletusi

Fauga faletusi

Fa'asologa

Fa'asologa

Iloiloga fa'amaumauga

Iloiloga fa'amaumauga

Fa'ata'ita'iga QC

Tuuina atu o poloketi


  • Muamua:
  • Sosoo ai:

  • Su'esu'ega Tulaga Tulaga o le DNA Fa'ata'ita'iga

    Laulau 1. Fa'amaumauga i fa'amaumauga mama.

    BMKID

    rawSeqNum

    rawSumBase

    cleanSeqNum

    cleanSumBase

    mamaN50Len

    mamaN90Len

    mamaMeanLen

    mamaMaxLen

    mamaMeanQual

    DNA_BMK01

    1,218,239

    26.37

    1,121,736

    25.90

    28,014

    15,764

    23,090

    143,181

    9

    Su'esu'ega Tulaga o Fa'amaumauga o Fa'ata'ita'iga RNA

    Laulau 1. Fa'amaumauga i fa'amaumauga mama.

    Igoa faila

    ClientID

    FaitauNumera

    BaseNumera

    N50

    Uiga Umi

    MaxLength

    MeanQscore

    RNA_BMK001

    C2

    8,947,708

    4,047,230,083

    398

    452

    129,227

    Q12

    Ata 1. Faitau le tufatufaga umi

    A3

    Ata 2. Tufatufaina togi lelei o fa'amaumauga mama

    A4

    Ata 3. Le umi ma le lelei o togi fa'asoaina o fa'amaumauga mama

    A5

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