Fa'avae | Tele o Faletusi | Fa'amatalaga Fa'amatalaga Fa'amatalaga (Per Sel) | Fa'asa'o Tutoatasi | Talosaga |
Nanopore | 8Kb, 10kb, 20kb, Ultralong, cDNA-PCR | 70-90Gb/Sele | 85-92% | SV vala'au, De novo, Fa'asologa atoa, Iso-Seq, Gene fa'amatalaga, Su'esu'eina ole DNA methylation |
● I luga o le 5 tausaga le poto masani i luga o le PacBio sequencing platform ma le faitau afe o galuega tapunia ma ituaiga eseese.
● BMKGENE ose paaga aloaʻia a Oxford Nanopore, faʻatasi ai ma faʻailoga lua RNA/DNA.
● O loʻo iai faʻataʻitaʻiga masani o faʻasologa faʻatasi ma meafaigaluega atoatoa ma lava faʻasologa faʻasologa.
● Faʻavae i luga o le Nanopore platform, e sili atu i le 10 manu ma laʻau suʻesuʻega Denovo ua lomia i tusitala faʻavaomalo lauiloa.
Ituaiga Fa'ata'ita'i | Aofaiga | Fa'atonuga(Qubit ®) | Volume | Mama | O isi |
Genomic DNA | Fa'alagolago i Fa'amatalaga Manaomia | ≥20ng/μl | ≥15μl | OD260/280=1.7-2.2; OD260/230≥1.5; Malamalama pito i luga ile 260 nm, leai se faʻaleagaina | E mana'omia le fuaina o le fa'atonuga e Qubit ma Qubit/Nanopore ≤ 2 |
RNA atoa | ≥1.2μg | ≥100μg/μl | ≥15μl | OD260/280=1.7-2.5; OD260/230=0.5-2.5;leai se fa'aleagaina | RIN tau ≥7.5 |
Su'esu'ega Tulaga Tulaga o le DNA Fa'ata'ita'iga
Laulau 1. Fa'amaumauga i fa'amaumauga mama.
BMKID | rawSeqNum | rawSumBase | cleanSeqNum | cleanSumBase | mamaN50Len | mamaN90Len | mamaMeanLen | mamaMaxLen | mamaMeanQual |
DNA_BMK01 | 1,218,239 | 26.37 | 1,121,736 | 25.90 | 28,014 | 15,764 | 23,090 | 143,181 | 9 |
Su'esu'ega Tulaga o Fa'amaumauga o Fa'ata'ita'iga RNA
Laulau 1. Fa'amaumauga i fa'amaumauga mama.
Igoa faila | ClientID | FaitauNumera | BaseNumera | N50 | Uiga Umi | MaxLength | MeanQscore |
RNA_BMK001 | C2 | 8,947,708 | 4,047,230,083 | 398 | 452 | 129,227 | Q12 |
Ata 1. Faitau le tufatufaga umi
Ata 2. Tufatufaina togi lelei o fa'amaumauga mama
Ata 3. Le umi ma le lelei o togi fa'asoaina o fa'amaumauga mama