●Fa'avae:Illumina NovaSeq 6000, NovaSeq, HiSeq X Ten ma le BGI-DNB-T7
●Fa'asologa o faiga:PE50, PE100, PE150, PE250
●Pulea lelei o faletusi a'o le'i fa'asologa
●Fa'asologa o fa'amatalaga tu'uina atu ma QC:tu'uina atu o le QC lipoti ma fa'amatalaga mata'utia i le fa'asologa vaveq pe a mae'a le fa'avasegaina ma le fa'amamaina o le Q30 faitau.
●Fetuuna'iga o auaunaga Fa'asologa:e mafai e le tagata fa'atau ona filifili e fa'asologa i le ala, tafe sela po'o le aofa'i o fa'amaumauga.
●Fetuuna'iga o tulaga:O faletusi DNB e mafai ona faʻafeiloaʻi i luga ole Illumina platforms
●Le poto masani i luga ole tulaga faʻasologa o le Illumina:fa'atasi ai ma le faitau afe o galuega tapuni ma ituaiga eseese.
●Tuuina atu o le fa'asologa o lipoti QC:fa'atasi ai ma fua fa'atatau, sa'o fa'amaumauga ma le fa'atinoga atoa o le fa'asologa o galuega.
●Fa'asologa fa'asologa o tagata matutua:fa'atasi ai ma sina taimi puupuu.
●Pulea lelei: matou te faʻatinoina manaʻoga QC e faʻamautinoa ai le tuʻuina atu o taunuʻuga maualuga maualuga.
Aofa'i Fa'amatalaga (X) | Fa'atotonuga (qPCR/nM) | Volume |
X ≤ 50 Gb | ≥ 2 nM | ≥ 20 μl |
50 Gb ≤ X <100 Gb | ≥ 3 nM | ≥ 20 μl |
X ≥ 100 Gb | ≥ 4 nM | ≥ 20 μl |
Fa'avae | Fa'atotonuga (qPCR/nM) | Volume |
HiSeq X Sefulu | ≥ 2 nM | ≥ 20 μl |
NovaSeq 6000 SP | ≥ 1 nM | ≥ 25 μl |
NovaSeq 6000 S4 | ≥ 1.5 nM | ≥ 25 μl |
NovaSeq X | ≥ 1.5 nM | ≥ 25 μl |
BGI-DNBSEQ-T7 | ≥ 1.5 nM | ≥ 25 μl |
I le faaopoopo atu i le faʻatonuga ma le aofaʻi atoa, e manaʻomia foʻi se faʻataʻitaʻiga maualuga talafeagai.
E tu'uina atu se lipoti i le tulaga lelei o le faletusi a'o le'i fa'asologa, iloilo le aofa'i o faletusi, ma vaevaega.
Laulau 1. Fa'amaumauga ile fa'asologa o fa'amaumauga.
Fa'ata'ita'iga ID | BMKID | Faitauga mata'utia | Fa'amatalaga Mata'utia (bp) | Faitau mama (%) | Q20(%) | Q30(%) | GC(%) |
C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Ata 1. Tu'ufa'atasiga lelei ma faitau i fa'ata'ita'iga ta'itasi
Ata 2. Tufatufaina o mataupu faavae
Ata 3. Tufatufaina o mataupu faitau ile fa'asologa o fa'amaumauga