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条形 fu'a-03

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Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)

Su'esu'ega mo le Transposase-Accessible Chromatin ma le fa'asologa maualuga (ATAC-seq) Ata Fa'aalia
  • Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)
  • Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)
  • Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)
  • Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)
  • Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)

ATAC-seq o se metotia faʻasolosolo maualuga mo le suʻesuʻeina o le genome-wide chromatin accessibility, lea e taua mo le pulea epigenetic lalolagi o faʻamatalaga o kenera.E fa'apipi'i fa'asologa fa'asologa i vaega o chromatin matala e ala ile Tn5 transposase.A maeʻa le PCR faʻateleina, e fausia se faletusi faʻasologa.O vaega uma o le chromatin e mafai ona maua i lalo o se tulaga avanoa-taimi fa'apitoa, e le gata i nofoaga o lo'o fusifusia ai se vaega fa'aliliuga, po'o se vaega fa'asologa fa'asolosolo.


Au'aunaga Fa'amatalaga

Bioinformatics

I'uga Fa'aaliga

Tulaga Auaunaga

● Su'esu'ega Atoa: Fa'apolofesa su'esu'ega paipa e fa'afetaui ai le tele o mana'oga fa'apitoa;

● Aafiaga Tele: E fiaselau galuega faatino ua maeʻa manuia i le taimi nei, e aofia ai manu ma laau faʻataʻitaʻi.

Fa'amatalaga Auaunaga

Tulaga: Illumina NovaSeq tulaga

Ituaiga faletusi: ATAC-seq

Fa'atonuga fa'amaumauga: ≥20M faitau

Fa'ata'ita'iga Manaoga

Tulaga fa'ata'ita'i: Tissue, sela ola, po'o sela fa'a'aisa ma isi

Numera sela: ≥ 5×105sela, o se numera sela sa'o o le ki lea i le manuia o le su'ega;

Tisu mamafa: ≥ 200mg mea fou;

Toto: ≥ 2 mL

Auala o Galuega Au'aunaga

Fa'ata'ita'iga QC

Fa'ata'ita'iga mamanu

fa'ata'ita'iga tu'uina atu

Fa'ata'ita'iga tu'uina atu

Fa'ata'ita'iga pailate

Su'ega DNA

Sauniuniga Faletusi

Fauga faletusi

Fa'asologa

Fa'asologa

Iloiloga fa'amaumauga

Iloiloga fa'amaumauga

A uma le fa'atauga Auaunaga

Au'aunaga pe a uma ona fa'atau

  • Muamua:
  • Sosoo ai:

    • 表观-02

    1. Heatmap ile ATAC faitau tufatufaga ile TSS ma itulagi lata ane (±3 kb)

    3（1）2.Distribution o le chromatin itulagi tatala i vaega genome eseese

    4(1)

    3. Differential tumutumu valaau i le va o vaega

    5(1)

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