Urubuga rwa PacBio rukurikirana ni urubuga rwasomwe igihe kirekire, ruzwi kandi nka bumwe mu buhanga bwa gatatu (TGS).Tekinoroji yibanze, molekile imwe-nyayo-nyayo (SMRT), iha imbaraga ibisekuruza byasomwe hamwe na kilo-shingiro muburebure.Hishimikijwe "Gukurikirana-by-Synthesis", gukemura nucleotide imwe bigerwaho na Zero-mode waveguide (ZMW), aho ijwi rito gusa hepfo (site ya synthesis ya molekile), rimurikirwa.Mubyongeyeho, urutonde rwa SMRT rwirinda ahanini kubogama kubogamye muri sisitemu ya NGS, kuberako intambwe nyinshi zo kongera PCR zidasabwa mubikorwa byo kubaka isomero.