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Gukurikirana Metagenomic-Nanopore
Metagenomics nigikoresho cya molekuline gikoreshwa mu gusesengura ibikoresho bivangwa na genomic bivanwa mu ngero z’ibidukikije, bitanga amakuru arambuye mu miterere y’ibinyabuzima no ku bwinshi, imiterere y’abaturage, isano ya phylogeneque, ingirabuzimafatizo ikora hamwe n’ibidukikije, n'ibindi. Kuri metagenomic.Imikorere yayo idasanzwe mubisomwa birebire byongerewe imbaraga zo gusesengura metagenomic, cyane cyane inteko ya metagenome.Ufashe ibyiza byo gusoma-uburebure, Nanopore ishingiye kuri metagenomic ubushakashatsi irashobora kugera ku nteko ikomeza ugereranije na metagenomics.Byashyizwe ahagaragara ko metagenomics ishingiye kuri Nanopore yabyaye genoside yuzuye kandi ifunze mikorobe (Moss, EL, et al.,Kamere ya Biotech, 2020)
Ihuriro ：Nanopore Isezerano P48
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Genome yose bisul fi te ikurikiranye
Methylation ya ADN kumwanya wa gatanu muri cytosine (5-mC) igira uruhare runini mumagambo ya gene no mubikorwa bya selile.Uburyo bwa methylation budasanzwe bwajyanye nibintu byinshi n'indwara nka kanseri.WGBS yahindutse zahabu yo kwiga genome-ubugari bwa methylation kumurongo umwe.
Ihuriro: Illumina NovaSeq6000
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Suzuma kuri Transposase-Yagerwaho Chromatin hamwe na Byinshi Byakurikiranye (ATAC-seq)
ATAC-seq nuburyo bukurikira bwo gutondekanya uburyo bwo gusesengura genome-yagutse ya chromatine, ifite akamaro kanini mugukwirakwiza epigenetike kwisi yose.Adapteri zikurikirana zinjizwa mukarere ka chromatin zifunguye na hyperactive Tn5 transposase.Nyuma ya PCR yongerewe imbaraga, hubatswe isomero rikurikirana.Uturere twose twa chromatin dufunguye turashobora kuboneka mugihe cyumwanya-wihariye, ntabwo bigarukira gusa kumwanya uhuza ibintu byandikirwa, cyangwa akarere runaka kahinduwe.
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16S / 18S / ITS Ikurikirana rya Amplicon-PacBio
Subunit kuri 16S na 18S rRNA ikubiyemo uturere twabitswe cyane hamwe na hyper-variable ni urutoki rwiza rwa molekuline kugirango tumenye ibinyabuzima bya prokaryotic na eukaryotic.Ukoresheje uburyo bukurikirana, izi amplicons zirashobora kwibasirwa hashingiwe kubice byabitswe kandi uturere twa hyper-variable turashobora kurangwa byuzuye kubiranga mikorobe bigira uruhare mubushakashatsi bukubiyemo isesengura ryimiterere ya mikorobe, tagisi, phylogene, nibindi. ) Urutonde rwa PacBio rushobora kubona ibisobanuro birebire bisomwa neza, bishobora gutwikira amplicons yuzuye (hafi 1.5 Kb).Kugura kwagutse k'umurima wa genetike byongereye cyane imiterere yo gutangaza amoko muri bagiteri cyangwa mugace ka fungi.
Ihuriro ：Urutonde rwa PacBio II
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16S / 18S / ITS Ikurikirana rya Amplicon-NGS
16S / 18S / ITS ikurikirana ya amplicon igamije kwerekana phylogene, tagisi, nubwinshi bwamoko mumuryango wa mikorobe ukoresheje iperereza ryibicuruzwa bya PCR byerekana ibimenyetso bikomoka kumurugo birimo ibice byaganiriweho cyane kandi birenze urugero.Kwinjiza kwintoki za molekuline nziza na Woeses et al, (1977) iha imbaraga microbiome yerekana umwirondoro.Urukurikirane rwa 16S (bagiteri), 18S (fungi) hamwe na spacer yimbere yimbere (ITS, fungi) ituma hamenyekana amoko menshi kimwe nubwoko budasanzwe kandi butamenyekana.Iri koranabuhanga ryahindutse igikoresho kinini kandi gikomeye muguhitamo mikorobe itandukanye mubidukikije, nkumunwa wabantu, amara, umwanda, nibindi.
Ihuriro ：Illumina NovaSeq6000
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Indwara ya bagiteri na fungal Yongeye gukurikiranwa
Indwara ya bagiteri na fungal yongeye gukurikiranwa nigikoresho gikomeye cyo kurangiza genome za bagiteri zizwi na fungi, ndetse no kugereranya genome nyinshi cyangwa gushushanya genome yibinyabuzima bishya.Ni ngombwa cyane gutondekanya genome zose za bagiteri na fungi kugirango tubyare genomisiyo nyayo, gukora mikorobe hamwe nubundi bushakashatsi bugereranya.
Ihuriro: Illumina NovaSeq 6000
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PacBio-Yuzuye-Transcriptome (Non-Reference)
Ihuriro rya Amplicon (16S / 18S / ITS) ryatejwe imbere nuburambe bwimyaka mu isesengura ryimishinga itandukanye ya mikorobe, ikubiyemo isesengura ryibanze ryibanze hamwe nisesengura ryihariye: isesengura ryibanze rikubiyemo isesengura ryibanze ryibintu bikubiye mubushakashatsi bwa mikorobe, ibisesengura birakungahaye kandi byuzuye, n'ibisubizo by'isesengura bitangwa muburyo bwa raporo z'umushinga;Ibiri mu isesengura ryihariye biratandukanye.Ingero zirashobora gutoranywa kandi ibipimo birashobora gushyirwaho byoroshye ukurikije raporo yibanze yisesengura nintego yubushakashatsi, kugirango tumenye ibisabwa byihariye.Sisitemu y'imikorere ya Windows, yoroshye kandi yihuse.
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PacBio-Yuzuye-Transcriptome (Non-Reference)
Gufata Biologiya ya Pasifika (PacBio) Isoform ikurikirana yamakuru nkayinjiza, iyi Porogaramu irashobora kumenya uburebure bwuzuye bwuzuye (nta guterana).Mugushushanya uburebure bwuzuye burwanya genome yerekanwe, inyandiko-mvugo irashobora gutezimbere na gen izwi, inyandiko-mvugo, uturere twa code, nibindi. Muri iki gihe, kumenya neza imiterere ya mRNA, nko gutondeka ubundi, nibindi, birashobora kugerwaho.Isesengura rifatanije hamwe na NGS inyandiko-mvugo ikurikirana ituma ibisobanuro birambuye hamwe no kugereranya neza mu mvugo kurwego rwinyandiko-mvugo, ibyo bikaba ahanini byungura imvugo itandukanye no gusesengura imikorere.
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Kugabanya Guhagararirwa Bisulfite Urukurikirane (RRBS)
Ubushakashatsi bwa methylation ya ADN buri gihe bwabaye ingingo ishyushye mubushakashatsi bwindwara, kandi bufitanye isano rya bugufi nimvugo ya gene hamwe na pheno-typic.RRBS nuburyo nyabwo, bukora neza kandi bwubukungu kubushakashatsi bwa methylation ya ADN.Gutunganya uturere twa CpG hamwe na CpG mukarere ka enzymatique (Msp I), hamwe na Bisulfite ikurikirana, bitanga ibisubizo bihanitse bya ADN methylation.
Ihuriro: Illumina NovaSeq 6000
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Prokaryotic RNA ikurikirana
Urutonde rwa Prokaryotic RNA rukoresha ibisekuruza bizakurikiraho (NGS) kugirango bigaragaze ko RNA ihari kandi ingano mugihe runaka, ukoresheje isesengura ryimiterere ya selile.Isosiyete yacu ya prokaryotic RNA ikurikirana, igamije cyane cyane kuri prokaryote hamwe na genome yerekana, iguha ibisobanuro byerekana inyandiko-mvugo, isesengura ryimiterere ya gene, nibindi byakoreshejwe cyane mubushakashatsi bwibanze bwa siyanse, ubushakashatsi bwibiyobyabwenge niterambere, nibindi byinshi.
Ihuriro: Illumina NovaSeq 6000
