GENOME EVOLUTION
Genome De novo
Inteko | Kugena igitsina Igikorwa cyose gikurikirana hamwe na bioinformatike igice cyatanzwe na Biomarker Techonologies.
Ibisobanuro
“Ikigereranyo cya fenotipike yinyanja zirimo imigereka imeze nkibabi, umunwa utagira amenyo, hamwe no gutwita kwabagabo birimo gutera intanga ngore kuri "pompe brood".We de novo - ikurikiranye genome yumugabo nigitsina gore yo mu nyanja rusange (Phyllopteryx taeniolatus) hamwe nubwoko bwayo bifitanye isano rya bugufi, imiyoboro ya alligator (Syngnathoides biaculeatus).Imyandikire yimyandikire ivuye mubintu bishya byabayeho biturutse ku bwihindurize, ibibabi bisa n’ibibabi, byerekana ko itsinda rya gen risanzwe rigira uruhare mu iterambere ry’imisoro ryahurijwe hamwe ndetse no gutunganya inyandiko za tran kugira ngo zishobore gusana ingirabuzimafatizo hamwe na gen.Imiterere ya zebrafish ya scpp5, yatakaye muri syngnathide zose, wasangaga ibura cyangwa yahinduye amenyo ya pharyngeal, ishyigikira hypothesis ivuga ko gutakaza scpp5 byagize uruhare mu gutakaza amenyo muri syngnathide.Igitsina gishyiraho - kugena inzige zirimo amhr2y yihariye yumugabo ihuriweho na seadragon hamwe na pipfish ya alligator yamenyekanye. ”
Ibikoresho nuburyo bukurikira:
Ibikoresho
Ficyuzi rusange (P. taeniolatus) na bibiri bya alligator pipefish (S. biaculeatus) ingero.Inyanja ebyiri zisanzwe (umwe wumugabo numugore umwe) hamwe na pisifike ebyiri za alligator (umugabo umwe numugore umwe) byakoreshwaga mugukurikirana genome yose, naho abandi bantu babiri basanzwe ba seadragon bakoreshwa muguhuza genome.
Ingamba zikurikirana
270/350 bp isomero (Hiseq 2500) + Pacbio 20Kb (Ibikurikira) cyangwa Nanopore 30 Kb (MinION) + Hi-C (NovaSeq 6000) .byakurikiyeho, nabandi bantu babiri basanzwe ba seadragon yabagabo yakoreshejwe muguhuza genome.
Urutonde rukurikirana
Fcyangwa seadragon isanzwe, ubwonko, ijisho, gill, umwijima, umutima, impyiko, mesentery, amara, imitsi, fin, uruhu, imigozi imeze nkibibabi, testis, na ovary.
Ingamba zo guteranya Genome de novo
Canu (Gukosora) + WTDBG2 (Inteko) + Pilon (Igipolonye) + Lachesis (Hi-C).
Ibisubizo Bikuru
Igishushanyo 1. Ibyingenzi byingenzi biranga ikiyaga rusange (P. taeniolatus) hamwe na pipfish ya alligator (S. biaculeatus) hamwe na phylogeneque yabo.
Igishushanyo cya 2
Igishushanyo 3. Gushiraho igitsina cyo gushira mu nyanja rusange (P. taeniolatus) hamwe na pipefish ya alligator (S. biaculeatus).
Igishushanyo cya 4. Fenotipi yinyo yinyo muri zebrafish scpp5 mutants.
Igihe cyo kohereza: Mutarama-08-2022