MICROBIAL
Guhuza na bagiteri hamwe na immobilisation hamwe na biochar byateje imbere kwangirika kwa tebuconazole, mikorobe yubutaka nibikorwa.
Uburebure bwuzuye 16S ikurikirana amplicon |PacBio Muraho |Alpha itandukanye |Ubwoko bwa Beta
Muri ubu bushakashatsi, uburebure bwa 16S amplicon ikurikirana na PacBio hamwe nisesengura rya bioinformatic byatanzwe na Biomarker Technologies.
Ingingo z'ingenzi
Biochar immobilized tebuconazole-yangiza za bagiteri Alcaligenes faecalis WZ-2 yakozwe ku mikorere ya biodegradation kandi igira ingaruka ku butaka bwanduye bwa tebuconazole ugereranije nubwoko butesha agaciro WZ-2.
1. Biochar-immobilized WZ-2 yerekanye iyangirika ryiza muri tebuconazole ugereranije na WZ-2 yubusa kugabanya tebuconazole igice cyubuzima bwubutaka kuva muminsi 18.7 kugeza kumunsi 13.3.
2. Biochar-immobilized WZ-2 yashoboye kugarura ibikorwa bya mikorobe yubutaka kavukire, harimo urease, dehydrogenase na invertase, nibindi.
3. Umwirondoro wa mikorobe muri biochar-immobilisation WZ-2 itunganijwe igenwa nuburebure bwa 16S ikurikirana yashyigikiye cyane ko iyi sisitemu ishobora kugarura ubuzima bwubutaka mugutezimbere imiterere ya bagiteri yanduye tebuconazole.
Inararibonye (Urukurikirane rujyanye)
Itsinda: CK: Ubutaka karemano;T: Ubutaka bwazengurutswe na tebuconazole;S: Tebuconazole yarimo ubutaka hamwe nubusa WZ-2;BC: Tebuconazole yarimo ubutaka hamwe na biochar;BCS: Tebuconazole yarimo ubutaka hamwe na biochar immobilized WZ-2.
Icyitegererezo: Gukuramo ubutaka bwose ADN yongerewe na 16S rDNA primers
27F (5′-AGAGTTTGATCCTGGCTCAG-3 ′) na 1492R (5′-GGTTACCTTGTTACGA),kwihana uburebure bwuzuye 16S rDNA
Urubuga rukurikirana: PacBio RS II
Ingamba zikurikirana: CCS HIFI irasoma
Isesengura ryamakuru:BMKCloudBioinformatic Platforming ituma amafi ya zahabu ari uburyo bwiza bwo kwerekana imiterere y amafi.
Igisubizo
Imiterere yubutaka bwa mikorobe yagenwe na 16S rDNA ikurikirana.Ubukire bwa OTU hamwe na alfa itandukanye, harimo Chang1, Ace, Shannon na Simpson byagaragaye kugirango hagaragazwe ubwoko butandukanye muri buri sisitemu.Nyuma yiminsi 60 incububasi, indice zose zerekanye ibintu bisa, ni ukuvuga tebuconazole bishobora kugabanya ubukire bwubwoko no gutandukana mubutaka.Ariko, wongeyeho imbaraga za WZ-2, umuganda wa bagiteri wubutaka wagaruwe igice ukurikije ubutunzi nubwinshi.Itandukaniro rito ryagaragaye muri BC, BCS na CK, byerekana ko biochar na biochar-immobilisation WZ-2 ishobora kugarura neza ubuzima bwibinyabuzima byubutaka.
Uburyo butaremereye Bwombi-Itsinda Uburyo hamwe na Arithmetic Means (UPGMA) bwakoreshejwe murubu bushakashatsi bwerekana beta itandukanye mumatsinda.Nkuko bigaragara ku gishushanyo gikurikira, BC, BSC na CK basangiye uburyo busa n’imiterere ya mikorobe ugereranije nitsinda T na S, byerekanaga kandi ko kwinjiza biochar muri tebuconazole yanduye bioremediation bishobora ahanini koroshya mikorobe mu butaka.
Igishushanyo.UPGMA ihuriro ryumuryango wa bagiteri kurwego rwa phylum mubuvuzi butandukanye
Reba
Izuba, Tong, n'abandi.Ati: "Guhuza kwa bagiteri no kudahangarwa hamwe na biochar byateje imbere iyangirika rya tebuconazole, imiterere ya mikorobe yubutaka n'imikorere."Ikinyamakuru c'ibikoresho biteye akaga398 (2020): 122941.
Amakuru n'ibikurubikuru igamije gusangira ibibazo byatsinzwe na Biomarker Technologies, gufata ibyagezweho mu buhanga hamwe nubuhanga bukomeye bwakoreshejwe mugihe cyo kwiga.
Igihe cyo kohereza: Mutarama-08-2022