GWAS
Umutwe: Gukurikirana-genome yose yerekana inkomoko ya Brassica napus na geneti ya genetike igira uruhare mugutezimbere
Ikinyamakuru: Itumanaho rya Kamere
NGS |WGS |Igisubizo |GWAS |Inyandiko |RNAseq |Brassica napus |Ubwihindurize |Kurera mu rugo
Muri ubu bushakashatsi, Biomarker Technologies yatanze Serivisi zikurikirana za NGS, hamwe n'inkunga ya tekiniki ku isesengura rya bioinformatics ku makuru akurikirana.
Amavu n'amavuko
Brassica napus.Nubwo bimeze bityo ariko, niba amoko yo mu gasozi cyangwa abaterankunga bororerwa mu rugo ari ababyeyi bakomokamo na gen byagize uruhare mu gutunga no gutera imbere ku ngufu kugeza ubu ntibiramenyekana.
Ibikoresho nuburyo
Ibikoresho:588B. napusabinjira bagize uruhare muri ubu bushakashatsi, barimo 466 bo muri Aziya, 102 bo mu Burayi, 13 bo muri Amerika ya Ruguru, na 7 bo muri Ositaraliya.Ukurikije inyandiko zo gukura, ibyo bikoresho byagabanijwemo ecotypes eshatu;amasoko (86 yinjira), imbeho (74 yinjira), na kimwe cya kabiri cy'itumba (428).
Urukurikirane:Ugereranyije.5 × (kuva kuri 3.37 × kugeza 7,71 ×)
Urubuga rukurikirana:Illumina Hiseq 4000
Gutanga amakuru:4.03 Tb amakuru meza
Guhamagara SNP:BWA + GATK.5.294.158 SNPs na 1,307.151 InDels zabonetse.
Ibisubizo
Inkomoko ya B. napus
B. napusSubgenome yahindutse uhereye kumukurambere wiburayi.Ikirangantego cya gene kuva i Burayi kugera kuriB. napus Subgenome yabayeho ~ 106–1170 ishize.B. napusC subgenome irashobora kuba yarahindutse kuva mubisekuruza bisanzwe byiyi mirongo.Abakurambere baB. napusgutandukana nabakurambere basanzwe ba B. oleracea, hamwe na gene iheruka kwinjiraB. napus~ Imyaka 108–898 ishize.B. napusC subgenome ifite inkomoko igoye kuruta A Subgenome.Icyuho gikomeye cyateye imbere muri subgenomes zombi mugiheB. napusubwihindurize.Igihe cy'itumba na kimwe cya kabiriB. napusecotypes yatandukanijwe ~ imyaka 60 ishize, mugihe itumba nimpeshyiB. napusgutandukana ~ 416 hashize, hamwe n'amavuta hamwe n'amavutaB. napusgutandukana ~ 277 ishize.
Igishushanyo cya 2 Imiterere yabaturage 588 B. napus yinjira na 199 ya B. rapa.
Igishushanyo cya 3 Imiterere yabaturage 588 B. napus yinjira na 119 ya B. oleracea
Ibimenyetso byo gutoranya hamwe na Genome-yagutse yiga.
Mugihe cyambere cyo kunoza (FSI), ubwoko butandukanye bwubwoko bwatakaye muri B. napus C subgenome kuruta muri A subgenome.Itandukaniro rishingiye ku gitsina ryabaye mugihe cya FSI kuruta mugihe cya kabiri cyo gutera imbere (SSI).Imirasire ya SSI-itoranya ibimenyetso byakungahaye muburyo bwo kwihanganira imihangayiko, iterambere n'inzira za metabolike.60 loci ifitanye isano cyane nibiranga intego 10, harimo 5 ijyanye numusaruro wimbuto, 3 kugeza kuri silique, 4 kuri peteroli, na 48 kumiterere yimbuto.
Igishushanyo cya 4 Genome-mugusikana hamwe no gutangaza uturere twatoranijwe mugihe cya SSI ya B. napus
Isesengura ryanditse
Amakuru ya RNASEQ yingingo 11 zirimo amavuta menshi kandi akubye kabiri-ubuhinzi buke hamwe namavuta make kandi yibasiwe na genes ebyiri zerekana inzira ya biosynthelatic yarenze urugero.
Igishushanyo cya 5 Incamake yindabyo-igihe muguhitamo ecotype kunoza B. napus
Ikiganiro
Ubu bushakashatsi bwatanze ibikoresho byingenzi byo gusobanukirwa inkomoko niterambere ryamateka yaB. napuskandi bizoroshya gutandukanya ishingiro ryimiterere yibintu byingenzi byubuhinzi.SNPs ihambaye ijyanye nibihinduka byiza, ibimenyetso byo gutoranya hamwe na genes zabakandida bizagira uruhare runini mugihe kizaza, cyane cyane mukuzamura umusaruro, ubwiza bwimbuto, ibirimo amavuta, hamwe no guhuza niki gihingwa cya allopolyploid hamwe na benewabo.
Reba
Ubwoko bwa genome bwuzuye bugaragaza inkomoko ya Brassica napus hamwe na geneti ya geneti igira uruhare mugutezimbere [J].Itumanaho rya Kamere, 2019, 10 (1).
Amakuru n'ibikurubikuru igamije gusangira ibibazo byatsinzwe na Biomarker Technologies, gufata ibyagezweho mu buhanga hamwe nubuhanga bukomeye bwakoreshejwe mugihe cyo kwiga.
Igihe cyo kohereza: Mutarama-05-2022