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Amakuru

GENOME EVOLUTION

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Kugereranya genome igereranya yerekana transposon-yunganirwa no kwaguka kwa genome hamwe nubwihindurize bwubwihindurize bwa 3D genomique muri pamba

Urukurikirane rwa Nanopore |Muraho-C |Urutonde rwa PacBio |Illumina |RNA ikurikirana |3 D ubwubatsi bwa genome |Transposon |Kugereranya genomics

Muri ubu bushakashatsi, Biomarker Technologies yatanze ubufasha bwa tekinike kuri Nanopore ikurikirana, Hi-C hamwe nisesengura rya bioinformatic.

Ibisobanuro

Kwiyongera kwingirakamaro (TE) byamenyekanye nkimbaraga zitera guhuza ingano ya genome no kwihindagurika, ariko ingaruka zo gukora imiterere ya 3D genomic ntizamenyekana mubimera.Hano, turatanga raporo-yerekana-genome iteraniro ryubwoko butatu bwipamba bingana inshuro eshatu mubunini bwa genome, aribyoGossypium rotundifolium(K2),G. arboreum(A2), naG. raimondii(D5), ukoresheje Oxford Nanopore Technologies.Kugereranya genome igereranya inyandiko irambuye kumurongo wihariye wa TE amplifisation igira uruhare runini mubunini bwa genome (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), ikanagaragaza ibirimo gene yabitswe hamwe nubusabane hagati ya genome.Twasanze hafi 17% ya genne ya syntenique yerekana ihinduka ryimiterere ya chromatin hagati yimikorere (“A”) hamwe nudakora (“B”), kandi TE amplification yajyanye no kwiyongera kwikigereranyo cya A mubice bya gene (~ 7,000) ) muri K2 na A2 ugereranije na D5.42% gusa byimiterere ya topologiya ihuza imipaka (TAD) yabitswe muri genome eshatu.Amakuru yacu yerekana kwongera kwa TE nyuma yo gushiraho imipaka yihariye ya TAD.Ubu bushakashatsi butanga uruhare rw'ubwiyongere bwa genoside ya transposon-ihindagurika mu ihindagurika ry'imiterere ya chromatine yo mu rwego rwo hejuru mu bimera.

Imibare yingenzi yo guteranya genome

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Igishushanyo.Inteko rusange hamwe nibisobanuro bya G. rotundifolium (K2)

Amakuru n'ibikurubikuru igamije gusangira ibibazo byatsinzwe na Biomarker Technologies, gufata ibyagezweho mu buhanga hamwe nubuhanga bukomeye bwakoreshejwe mugihe cyo kwiga.


Igihe cyo kohereza: Mutarama-05-2022

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