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Amakuru

Ubwoko bwa genotyping-cyane cyane kubantu benshi, nintambwe yingenzi mubushakashatsi bwamashyirahamwe, butanga ishingiro ryimiterere yo kuvumbura gene ikora, gusesengura ubwihindurize, nibindi. ) yatangijwe kugirango igabanye igiciro gikurikiranye kuri sample, mugihe gikomeza gukora neza kubuvumbuzi bwa geneti.Ibi mubisanzwe bigerwaho mugukuramo ibice byabuzanyijwe mubunini bwatanzwe, ibyo bikaba byaragabanijwe isomero ryerekana (RRL).Ibice byihariye-byongeweho ibice bikurikirana (SLAF-Seq) nuburyo bwateguwe bwo kuvumbura de novo SNP hamwe na genotyping ya SNP yabantu benshi.

Ibikorwa bya tekiniki

SLAF-tech-flow
SLAF-Seq-workflow-1011x1024

SLAF vs Uburyo bwa RRL buriho

SLAF

Ibyiza bya SLAF

Ikirangantego cyo hejuru cyo kuvumbura neza- Hamwe na tekinoroji ikurikirana cyane, SLAF-Seq irashobora kugera ku bihumbi amagana bya tagi yavumbuwe muri genome yose kugirango isohoze icyifuzo cyimishinga itandukanye yubushakashatsi, haba hamwe na genome yerekanwe.

Igishushanyo mbonera cyoroshye- Kubwintego zitandukanye zubushakashatsi cyangwa ubwoko, ingamba zinyuranye zo gusya zirahari harimo-enzyme imwe, enzyme-ebyiri na enzyme nyinshi.Ingamba zo gusya zizasuzumwa mbere muri silico kugirango tumenye neza igishushanyo mbonera cya enzyme.

Gukora neza murwego rwo gusya- Igenamigambi ryateguwe neza ritanga SLAFs iringaniye kuri chromosome.Gukusanya ibice neza birashobora kugera kuri 95%.

Irinde gusubiramo- Ijanisha ryikurikiranya ryisubiramo mumibare ya SLAF-Seq ryaragabanutse kugera munsi ya 5%, cyane cyane mubinyabuzima bifite urwego rwo hejuru rwibintu bisubiramo, nk'ingano, ibigori, nibindi.

Kwikorera wenyine bioinformatic workflow- BMK yateje imbere ibikorwa bya bioinformatike ikoreshwa mubikorwa bya tekinoroji ya SLAF-Seq kugirango yizere neza kandi neza nibisohoka byanyuma.

Ikoreshwa rya SLAF

Ikarita ya genetike

Ikarita yerekana ubwinshi bwikarita yubaka no kumenyekanisha loci igenzura imiterere yindabyo muri Chrysanthemum (Chrysanthemum x morifolium Ramat.)

Ikinyamakuru: Ubushakashatsi bwimbuto bwimbuto bwatangajwe: 2020.7

GWAS

Kumenyekanisha umukandida gene ijyanye nibirimo isofavone mu mbuto za soya ukoresheje genome-rugari hamwe no guhuza amakarita

Ikinyamakuru: Ikinyamakuru cyibimera cyasohotse: 2020.08

Ubwihindurize

Isesengura rusange rya genomic hamwe na de novo inteko irerekana inkomoko yumuceri wibyatsi nkumukino wubwihindurize

Ikinyamakuru: Ibimera bya Molecular Byatangajwe: 2019.5

Isesengura ryinshi rya Segregant (BSA)

GmST1, ikubiyemo sulfotransferase, itanga imbaraga zo kurwanya virusi ya soya ya mosaic ya G2 na G3

Ikinyamakuru: Ibimera, Akagari & Ibidukikije Byatangajwe: 2021.04

SLAF-BSA

Reba

Izuba X, Liu D, Zhang X, n'abandi.SLAF-Seq: uburyo bunoze bwo kuvumbura nini-nini ya SNP kuvumbura no gukoresha genotyping ukoresheje ibintu byinshi byinjira [J].Ikibanza kimwe, 2013, 8 (3): e58700
Indirimbo X, Xu Y, Gao K, n'abandi.Ikarita yerekana ubwinshi bwikarita yubaka no kumenyekanisha loci igenzura imiterere yindabyo muri Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.2020; 7: 108.
Wu D, Li D, Zhao X, n'abandi.Kumenyekanisha umukandida gene ifitanye isano nibirimo bya isoflavone mumbuto ya soya ukoresheje genome-rugari hamwe no guhuza amakarita.Igiterwa J. 2020;104 (4): 950-963.
Izuba J, Ma D, Tang L, n'abandi.Isesengura rusange ryabaturage hamwe na De Novo Inteko Yerekana Inkomoko yumuceri wibyatsi nkumukino wubwihindurize.Ibimera.2019; 12 (5): 632-647.Ibimera.2018;11 (11): 1360-1376.
Zhao X, Jing Y, Luo Z, n'abandi.GmST1, igizwe na sulfotransferase, itanga imbaraga zo kurwanya virusi ya soya ya G2 na G3.Gutera Ibidukikije.2021; 10.1111 / pce.14066


Igihe cyo kohereza: Mutarama-04-2022

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