GENOME EVOLUTION
imiterere y'ibinyabuzima
Iteraniro ryiza rya genome ryerekana imiterere ya genome hamwe na gen
PacBio |Illumina |Ikarita ya Bionano Ikarita |Muraho-C Inteko rusange |Ikarita ya genetike |Gutoranya Guhitamo |RNA-Seq |ISO-Seq |UMUKUNZI
Biomarker Technologies yatanze ubufasha bwa tekiniki kuri gahunda ya Pacbio, Urutonde rwa Hi-C hamwe nisesengura ryamakuru muri ubu bushakashatsi.
Ingingo z'ingenzi
1.Ubwa mbere bwa chromosomal-urwego rwohejuru Rye genome yabonetse, ifite ubunini bwa chromosome imwe irenze 1 Gb.
2. Ugereranije na gen, Tu, Aet na Hv, ibintu bidasanzwe bya LTR-RT byagaragaye muri genome ya Rye, ishinzwe kwagura ingano ya genome.
3.Gutandukana hagati yingano na diploid byabaye nyuma yo gutandukanya sayiri ningano, hamwe nigihe cyo gutandukana kubintu byombi bigera kuri 9.6 na 15 MYA.
FT genes ya fosifora irashobora kugenzura imiterere yumutwe hakiri kare.
4.Isesengura ryatoranijwe ryerekana uruhare rwa ScID1 mugutondekanya itariki yumutwe hamwe nibishoboka guhitamo kubitunga muri rye
Amavu n'amavuko
Amavu n'amavuko
Rye ni igihingwa cyagaciro nigihingwa cyubwatsi, umutungo wingenzi wogutunganya ingano na triticale, hamwe nibikoresho byingirakamaro mubushakashatsi bwa genomika bugereranije mubyatsi.Weining rye, ubwoko bwururabyo rwambere rwahinzwe mubushinwa, ni indashyikirwa kubera ko rwinshi rwinshi rwangiza ifu nifu ya stripe.Kugira ngo dusobanukirwe n'irondakoko hamwe na molekuline biranga imiterere y'indobanure no guteza imbere ubushakashatsi bwa genomic n'ubworozi mu ngano n'ibihingwa bifitanye isano, hano twakurikiranye tunasesengura genome ya Weining rye.
Ibyagezweho
Rye Genome
Genome ya Rye yubatswe muguhuza PacBio SMRT isoma, gusoma-Illumina isomwe mugihe gito, kimwe nibyavuye muri chromatin ihinduka (Hi-C), ikarita ya genetike, hamwe nisesengura rya BioNano.Ibiterane byakusanyirijwe hamwe (7,74 Gb) bingana na 98.47% yubunini bwa genome (7.86 Gb), hamwe na 93.67% byabapiganwe (7.25 Gb) bahabwa chromosome ndwi.Ibintu bisubiramo bigizwe na 90.31% ya genome yateranijwe.
Rye Genome
Ikarita ihuza abantu (WJ) yateje imbere ikoresheje ibimera 295 F2 biva mu kwambukiranya ubutaka bubiri (Weining × Jingzhou)
Ikarita ya Hi-C yerekana ikarita irindwi yateranijwe Weining rye chromosomes (1R - 7R)
Guhuza chromosomes zirindwi ziteranijwe za Weining rye hamwe nitsinda ririndwi ryihuza ryateje imbere ukoresheje Lo7 x Lo255 RIL
Indangantego ya LTR (LAI) agaciro ka genome ya Rye wasangaga 18.42 naho 1,393 (96,74%) ya genoside ya BUSCO yabungabunzwe cyane 1,440 .Ibisubizo byerekana ko urutonde rwa Weining rukurikirana rufite ubuziranenge murwego rwombi. n'uturere twa genic.Hafi ya 86,991 za poroteyine-coding, harimo 45,596 zifite ibyiringiro byinshi (HC) hamwe na 41,395 genes zidafite icyizere (LC).
2. Isesengura rya TE
Isesengura rya TE.Bose hamwe 6.99 Gb, bangana na 90.31% yinteko ya Weining, yatangajwe nka TE, irimo ibintu 2.671.941 byimiryango 537.Ibiri muri TE byari hejuru cyane kuruta ibyavuzwe mbere kuri Ta (84,70%), Tu (81.42%), Aet (84.40%), WEW (82.20%), cyangwa Hv (80.80%).Terminal ndende isubiramo retrotransposons (LTR-RTs), harimo Gypsy, Copia hamwe na RT idafite ibyiciro bya RT, niyo yiganjemo TE, kandi 1ccupi 84.49% yibisobanuro bya TE hamwe na 76.29% bya Weining genome;CACTA ADN ya transposons yari iya kabiri ya TE nyinshi cyane, igizwe na 11,68% yibirimo TE hamwe na 10.55% bya Weining genome.
Isesengura ryibintu bya transposon ya rye
Weining rye yari ifite umubare munini ugereranije winjiza muri LTR-RTs hamwe nimpinga ya amplification yagaragaye hashize imyaka miriyoni 0.5 (MYA), ikaba yari iheruka mubwoko bune;iyindi mpinga, yabayeho hafi 1.7 MYA, yarashaje kandi igaragara no muri sayiri.Kurwego ruhebuje, habonetse ibintu byinshi bya Copia muri Weining rye kuri 0.3 MYA, mugihe amplification ya Gypsy RTs yiganjemo uburyo bwo gukwirakwiza bimodal ya LTR-RT iturika.
3. Iperereza ryihindagurika rya genome hamwe na chromosome
Itandukaniro riri hagati yingano na diploid ryabaye nyuma yo gutandukanya sayiri ningano, hamwe nigihe cyo gutandukana kubintu byombi bigera kuri 9.6 na 15 MYA.1R, 2R, 3R byari collinear rwose hamwe na chromosomes ya 1, 2 na 3 za chromosomes.4R, 5R, 6R, 7R wasangaga hariho ibinini binini hamwe nibice.
4. Gusesengura kwigana gene n'ingaruka zabyo kuri genoside biosynthesis
Ikigaragara ni uko umubare wa gen zigizwe hamwe (TDGs) hamwe na gen zigereranijwe hafi ya (PDGs) za Weining rye byombi byari byinshi kuruta ibya Tu, Aet, Hv, Bd na Os.Transposed genes duplicated genes (TrDGs) nazo zari nyinshi kurenza izisanzwe kuri Tu na Aet.Kwagura genome ya Rye iherekejwe numubare munini wa duplications.Ubwiyongere bwa TE buturika muri rye bishobora kuba byaratumye umubare munini wa TrDGs.
Ubwihindurize hamwe na chromosome synteny isesengura rya genome ya rye
Isesengura ryikwirakwizwa rya gene ningaruka zabyo muburyo butandukanye bwa krahisi biosynthesis ifitanye isano na gen (SBRGs)
5. Gutandukanya intungamubiri zo kubika imbuto za rye (SSP) gene loci
Inzira enye za chromosomal (Sec-1 kugeza Sec-4) zerekana ingano SSPs zamenyekanye kuri 1R cyangwa 2R.gen-gliadin genes zahinduwe vuba aha ingano nubwoko bufitanye isano rya hafi nyuma yo gutandukanya ingano ningano.
6. Gusuzuma ibintu byandikirwa (TF) hamwe na gen zirwanya indwara
Isesengura rya rye secalin loci
Weining rye yari ifite indwara nyinshi zirwanya indwara (DRA) (1.989, Amakuru yinyongera 3) kurusha Tu (1,621), Aet (1,758), Hv (1,508), Bd (1,178), Os (1,575), na A (1.836) ), B (1,728) na D (1,888) subgenomes yingano zisanzwe.
7. Iperereza ryerekana imiterere ya gene ijyanye nimitwe ya kare
Imirasire ibiri ya FT ifite imvugo ndende mugihe cyumunsi, ScFT1 na ScFT2, byasobanuwe muri Weining genome.Ibisigarira bibiri bya aside amine ya ScFT2 (S76 na T132) fosifora yabonetse bifitanye isano no kugabanya igihe.
Iterambere hamwe na gene imvugo iranga ijyanye nimitwe yambere ya Weining rye
8. Ubucukuzi bw'uturere twa chromosomal na loci bishobora kugira uruhare mu gutunga ingano
SNPs zose hamwe 123,647 zakoreshejwe mugukora isesengura rya selevtive yohanagura hagati yimbuto zahinzwe na S. vavilovii.Ibimenyetso 11 byatoranijwe byerekanwe no kugabanya (DRI), icyerekezo cyo gukosora (FST) nuburyo bwa XP-CLR.ScID1 yasanze uruhare rushoboka mugutunganya itariki yumutwe.
Kumenya no gusesengura uturere twa chromosomal na loci bishobora kuba bifitanye isano no gutunga ingano
Reba
Li GW n'abandi.Iteraniro ryiza rya genome ryerekana imiterere ya genome hamwe na gen.Imiterere ya Kamere (2021)
Amakuru n'ibikurubikuru igamije gusangira ibibazo byatsinzwe na Biomarker Technologies, gufata ibyagezweho mu buhanga hamwe nubuhanga bukomeye bwakoreshejwe mugihe cyo kwiga.
Igihe cyo kohereza: Mutarama-05-2022