Kugereranya Genomika
Inyungu za serivisi
ØIsesengura ryuzuye ryuzuye, ririmo umunani ukunze gusesengurwa
ØKwizerwa kwinshi mubisesengura hamwe nibisobanuro birambuye kandi byoroshye kumvikana kubisubizo
ØByateguwe neza biteguye-gutangaza imibare
ØItsinda ryabahanga cyane bioinformatics ryujuje ibyifuzo bitandukanye byisesengura
ØIgihe gito cyo guhinduranya igihe hamwe nukuri neza mubisesengura;
ØUbunararibonye bwinshi hamwe ninshuro zirenga 90 zatsinze hamwe nibintu byinshi byashyizwe ahagaragara ingaruka zirenga 900;
Ibisobanuro bya serivisi
| Bigereranijwe guhindukira | Umubare wubwoko | Isesengura |
| Iminsi 30 y'akazi | 6 - 12 | Imiryango ikomokamo Kwiyongera kwumuryango no kugabanuka Kubaka ibiti bya phylogeneque Ikigereranyo cyigihe cyo kugereranya (Calibration ya Fossil isabwa) Igihe cyo kwinjiza LTR (Kubimera) Kwigana genome yose (Kubimera) Umuvuduko wo guhitamo Isesengura |
Isesengura rya bioinformatics
üUmuryango wa Gene
üPhylogenetics
üIgihe cyo gutandukana
üUmuvuduko wo guhitamo
üIsesengura
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
Tissue cyangwa ADN kugirango bikurikirane genome no guterana
Ku nyamaswa:
Imitsi (Ikonje): ≥ 2 g;
Amaraso yose (Ntabwo akonje): ml 2 ml (kubitabo 3)
Ku bimera:
1.Imbuto (urugero ingemwe zikiri nto zihingwa mu mbuto, ingemwe z'umuco wa tissue, nibindi): gukusanya amababi yose hamwe nigiti gito;
2.Ibihingwa bikuze: gukusanya uruti rwibiti hamwe namababi mashya 1 cyangwa 2 yambere yegereye uruti.
* Amafaranga asabwa kuri buri sample ≥ 4 g.(Imikoreshereze yuburyo bwo kubaka isomero ni 1 g kuri buri somero).
Kuri ADN:
| Ihuriro | Umubare | Kwibanda | Isuku |
| Nanopore | ≥10 μg | ≥ 150 ng / μL | OD260 / 280 = 1.6-2.5Ibisubizo bisobanutse nezaOn-gel: Sobanura neza hamwe no kwangirika gukabije. |
| PacBio (CCS) | ≥15 μg | ≥ 90 ng / μL |
Amakuru
Amadosiye akurikirana ya genome (.fasta) hamwe na dosiye yo gutangaza (.gff3) yubwoko bufitanye isano
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
*Ibisubizo byerekanwa hano byose biva muri genomes yatangajwe na Biomarker Technologies
1.LTR shyiramo igihe cyo kugereranya: Igishushanyo cyerekanaga ikwirakwizwa rya bimodal idasanzwe mugihe cyo kwinjiza LTR-RTs muri genome ya Weining, ugereranije nandi moko.Impinga iheruka kugaragara hashize imyaka miriyoni 0.5.
Li Guang n'abandi.,Kamere Kamere, 2021
2.Isesengura ryumuryango na gene kuri chayote (Sechium edule): Mu gusesengura chayote nandi moko 13 afitanye isano mumiryango ya gene, wasangaga Chayote ifitanye isano rya bugufi ninzoka (Trichosanthes anguina).Chayote ikomoka ku nzoka hafi ya 27-45 Mya no kwigana genome yose (WGD) byagaragaye muri chayote muri 25 ± 4 Mya, bikaba bibaye ubwa gatatu WGD muri cucuibitaceae.
Fu A n'abandi.,Ubushakashatsi bwimbuto, 2021
3.Isesengura rya sintete: genes zimwe na zimwe zijyanye na phytohormone mugutezimbere imbuto zabonetse muri chayote, inzoka ninzoka.Isano iri hagati ya chayote na squash irarenze gato hagati ya chayote ninzoka.
Fu A n'abandi.,Ubushakashatsi bwimbuto, 2021
4.Isesengura ryumuryango: KEGG itunganyiriza kwaguka kwumuryango no kugabanuka muri genth ya G.thurberi na G.davidsonii yerekanye ko genoside ya steroid biosynthesis na brassinosteroid biosynthesis yaguye.
Yang Z n'abandi.,Ibinyabuzima bya BMC, 2021
5.Isesengura ryuzuye rya genome: 4DTV na Ks isesengura ryerekanwe ibyabaye byose byo kwigana.Impinga ya intraspecies yerekanwe kwigana ibyabaye.Impinga ya intercecies yerekanwe ibintu byabaye.Isesengura ryerekanye ko ugereranije nandi moko atatu afitanye isano rya bugufi, O. europaea yanyuze muri gene nini cyane.
Rao G n'abandi.,Ubushakashatsi bwimbuto, 2021
Urubanza rwa BMK
Roza idafite ibishishwa: ubushishozi bwa genomique bujyanye no kurwanya imiterere
Byatangajwe: Isubiramo ry'ubumenyi bw'igihugu, 2021
Ingamba zikurikirana:
'Basye'sIhwa'(R.Wichurainan) genome:
Hafi.93 X PacBio + hafi.90 X Nanopore + 267 X Illumina
Ibisubizo by'ingenzi
1.Ubuzima bwiza bwa R.wichuraiana bwubatswe hakoreshejwe uburyo bukurikiranye bwo gusoma bukurikirana, butanga inteko ya 530.07 Mb (Ikigereranyo cya genome cyagereranijwe hafi 525.9 Mb na cytometrie na 525.5 nubushakashatsi bwakozwe na genome et Heterozygosity yari 1.03%).BUSCO yagereranije amanota 93.9%.Ugereranije na “Old blush” (haploOB), ubuziranenge n'ubwuzuzanye bw'iyi genome byemejwe na base base-base verisiyo hamwe na LTR yo guterana (LAI = 20.03).Genwich ya R.wichuraiana irimo 32,674 proteine coding genes.
2.Multi-omics isesengura rihuriweho, rigizwe na genomique igereranya, transcriptomics, QTL isesengura ryabaturage, ryagaragaje imvugo ikomeye hagati ya R. wichuraiana na Rosa chinensis.Na none, imvugo itandukanya genes zifitanye isano muri QTL byashobokaga kuba bifitanye isano no gushushanya ibiti.
Kugereranya genomics igereranya hagati ya Basye; s Thornless na Rosa chinensis harimo isesengura rya synteny, cluster ya gene, kwaguka no gusesengura ibintu, byagaragaje umubare munini wubwoko butandukanye, bujyanye nibintu byingenzi mumaroza.Kwaguka kudasanzwe muri NAC na FAR1 / FRS umuryango wa gene wasangaga bifitanye isano no kurwanya ibibara.
Kugereranya genomics igereranya hagati ya BT na haploOB.
Zhong, M., n'abandi.“Roza idafite uburibwe: ubushishozi bwa genomique bujyanye no kurwanya imvura”Isubiramo ry'ubumenyi bw'igihugu, 2021;, nwab092.
