Isesengura ryinshi rya Segregant
Inyungu za serivisi
Takagi n'abandi.,Ikinyamakuru cyibimera, 2013
ØAhantu heza: Kuvanga ibinini hamwe na 30 + 30 kugeza 200 + 200 kugirango ugabanye urusaku rwimbere;mutatants-mutatants-ishingiye kubakandida mukarere guhanura.
ØIsesengura ryuzuye: Ibisobanuro byimbitse byabakandida gene itangazo, harimo NR, SwissProt, GO, KEGG, COG, KOG, nibindi.
ØIgihe cyihuta cyane: Kwihuta kwa gene muminsi 45 yakazi.
ØUbunararibonye bunini: BMK yagize uruhare mubihumbi n'ibihumbi biranga aho ikorera, ikubiyemo ubwoko butandukanye nk'ibihingwa, ibikomoka ku mazi, amashyamba, indabyo, imbuto, n'ibindi.
Ibisobanuro bya serivisi
Abaturage:
Gutandukanya urubyaro rwababyeyi hamwe na phenotypes irwanya.
urugero urubyaro rwa F2, Gusubira inyuma (BC), Recombinant inbred line (RIL)
Kuvanga pisine
Kubiranga ubuziranenge: abantu 30 kugeza kuri 50 (byibuze 20) / byinshi
Kubintu byinshi: hejuru ya 5% kugeza 10% kubantu bafite phenotypes ikabije mubaturage bose (byibuze 30 + 30).
Basabwe uburebure bwimbitse
Nibura 20X / umubyeyi na 1X / urubyaro umuntu ku giti cye (urugero nko kuvanga urubyaro rwa 30 + 30 kugiti cye, ubujyakuzimu buzaba 30X kuri byinshi)
Isesengura rya bioinformatics
üGenome yuzuye
üGutunganya amakuru
üGuhamagara SNP / Indel
üKwerekana akarere k'abakandida
üUmukandida gene imikorere yo gutangaza
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
| icyitegererezo cya gDNA | Icyitegererezo |
| Kwibanda: ≥30 ng / μl | Ibimera: 1-2 g |
| Umubare: ≥2 μg (Inkingi ≥15 μl) | Inyamaswa: 0.5-1 g |
| Isuku: OD260 / 280 = 1.6-2.5 | Amaraso yose: 1.5 ml |
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
1.Ishyirahamwe ryisesengura ryibanze kuri Euclidean Intera (ED) kugirango umenye akarere k'abakandida.Mu ishusho ikurikira
X-axis: Umubare wa Chromosome;Buri kadomo kagaragaza ED agaciro ka SNP.Umurongo wirabura uhuye nagaciro ka ED.Agaciro kari hejuru ya ED yerekana isano ikomeye hagati yurubuga na phenotype.Umurongo utukura ugereranya urwego rwingenzi.
2.Isesengura ryishyirahamwe rishingiye kuri SNP-indangagaciro
X-axis: Umubare wa Chromosome;Buri kadomo kagaragaza SNP-indangagaciro.Umurongo wirabura ugereranya SNP-indangagaciro.Ninini agaciro ni, niko ishyirahamwe rifite akamaro.
Urubanza rwa BMK
Ingaruka nyamukuru yibintu biranga Fnl7.1 ikubiyemo intungamubiri itinze ya poroteyine nyinshi ijyanye n'uburebure bw'ijosi mu mbuto.
Byatangajwe: Gutera Ikinyamakuru Ikoranabuhanga, 2020
Ingamba zikurikirana:
Ababyeyi (Jin5-508, YN): Genome yose ihwanye na 34 × na 20 ×.
Ibidendezi bya ADN (50 Ijosi rirerire na 50 bigufi-bigufi): Ibisabwa kuri 61 × na 52 ×
Ibisubizo by'ingenzi
Muri ubu bushakashatsi, gutandukanya abaturage (F2 na F2: 3) byatewe no kwambukiranya umurongo wa combre ndende Jin5-508 na YN-ijosi rigufi.Ibidendezi bibiri bya ADN byubatswe nabantu 50 bakabije bafite ijosi rirerire hamwe nabantu 50 bakabije.Ingaruka-nyamukuru QTL yamenyekanye kuri Chr07 nisesengura rya BSA hamwe no gushushanya QTL gakondo.Agace k'abakandida karushijeho kugabanywa no gushushanya neza, kugereranya gene no kugereranya transgenji, byagaragaje gene y'ingenzi mu kugenzura uburebure bw'ijosi, CsFnl7.1.Mubyongeyeho, polymorphism muri CsFnl7.1 akarere ka promoteri wasangaga bifitanye isano nimvugo ihuye.Ubundi isesengura rya phylogeneque ryerekanaga ko Fnl7.1 inzige ishobora guturuka mubuhinde.
 QTL-ikarita mu isesengura rya BSA kugirango umenye akarere kandida kajyanye n'uburebure bw'ijosi |  LOD imyirondoro yuburebure bwa QTL yamenyekanye kuri Chr07 |
Xu, X., n'abandi.“Ingaruka nyamukuru iranga inzige Fnl7.1 ikubiyemo intungamubiri zitinze zifitanye isano n'uburebure bw'ijosi mu mbuto.”Gutera ibinyabuzima byikoranabuhanga Ikinyamakuru 18.7 (2020).
