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PacBio-Yuzuye-Transcriptome (Non-Reference)
Ihuriro rya Amplicon (16S / 18S / ITS) ryatejwe imbere nuburambe bwimyaka mu isesengura ryimishinga itandukanye ya mikorobe, ikubiyemo isesengura ryibanze ryibanze hamwe nisesengura ryihariye: isesengura ryibanze rikubiyemo isesengura ryibanze ryibintu bikubiye mubushakashatsi bwa mikorobe, ibisesengura birakungahaye kandi byuzuye, n'ibisubizo by'isesengura bitangwa muburyo bwa raporo z'umushinga;Ibiri mu isesengura ryihariye biratandukanye.Ingero zirashobora gutoranywa kandi ibipimo birashobora gushyirwaho byoroshye ukurikije raporo yibanze yisesengura nintego yubushakashatsi, kugirango tumenye ibisabwa byihariye.Sisitemu y'imikorere ya Windows, yoroshye kandi yihuse.
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PacBio-Yuzuye-Transcriptome (Non-Reference)
Gufata Biologiya ya Pasifika (PacBio) Isoform ikurikirana yamakuru nkayinjiza, iyi Porogaramu irashobora kumenya uburebure bwuzuye bwuzuye (nta guterana).Mugushushanya uburebure bwuzuye burwanya genome yerekanwe, inyandiko-mvugo irashobora gutezimbere na gen izwi, inyandiko-mvugo, uturere twa code, nibindi. Muri iki gihe, kumenya neza imiterere ya mRNA, nko gutondeka ubundi, nibindi, birashobora kugerwaho.Isesengura rifatanije hamwe na NGS inyandiko-mvugo ikurikirana ituma ibisobanuro birambuye hamwe no kugereranya neza mu mvugo kurwego rwinyandiko-mvugo, ibyo bikaba ahanini byungura imvugo itandukanye no gusesengura imikorere.
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Ibikoresho
BMKCloud ni urubuga ruyobora bioinformatike rutanga igisubizo kimwe kuri gahunda ya genomic, ikaba yizewe cyane nabashakashatsi mubyiciro bitandukanye birimo ubuvuzi, ubuhinzi, ibidukikije, nibindi. , ibikoresho byo kubara, ububiko rusange, amasomo ya bioinformtic kumurongo, nibindi. gusesengura, n'ibindi.
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RNA nto
RNA ntoya ni ubwoko bwa RNA itagufi ifite uburebure bwa 18-30 nt, harimo miRNA, siRNA na piRNA.Izi RNA ntoya zavuzwe cyane ko zigira uruhare mubikorwa bitandukanye byibinyabuzima nko kwangirika kwa mRNA, kubuza guhindura, gushinga heterochromatine, nibindi. urubuga rwo gusesengura rugizwe nisesengura risanzwe hamwe no gucukura amakuru yambere.Ukurikije amakuru ya RNA-seq, isesengura risanzwe rishobora kugera kuri miRNA kumenyekanisha no guhanura, miRNA intego yo guhanura gene, gutangaza no gusesengura imvugo.Isesengura ryambere rifasha gushakisha miRNA yihariye no kuyikuramo, igishushanyo mbonera cya Venn, miRNA hamwe no kubaka urusobe rwa gene.
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NGS-WGS (Illumina / BGI)
NGS-WGS ni genome yuzuye yo gusesengura urutonde, ikorwa hashingiwe kuburambe bukomeye muri Biomarker Technologies.Iyi porogaramu yoroshye-yo-gukoresha itanga uburyo bwihuse bwo gukora isesengura ryibikorwa byogushiraho gusa ibice bike byibanze, bihuye namakuru akurikirana ya ADN yaturutse kumurongo wa Illumina hamwe na BGI ikurikirana.Ihuriro ryashyizwe kumurongo wo hejuru wa mudasobwa, itanga imbaraga zo gusesengura amakuru neza mugihe gito cyane.Ubucukuzi bwamakuru yihariye burahari hashingiwe kubisesengura bisanzwe, harimo ikibazo cya gene ihindagurika, igishushanyo mbonera cya PCR, nibindi.
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mRNA (Reba)
Transcriptome ni ihuriro hagati yamakuru ya genomic na proteome yimikorere yibinyabuzima.Urwego rwo kwandukura urwego ningirakamaro kandi rwizwe cyane nuburyo bwo kugenzura ibinyabuzima.Urutonde rwa transcriptome rushobora gutondekanya inyandiko-mvugo umwanya uwariwo wose mugihe cyangwa mubihe byose, hamwe nicyemezo kiboneye kuri nucleotide imwe.Birashobora kwerekana imbaraga murwego rwo kwandukura gene, icyarimwe kumenya no kugereranya inyandiko zidasanzwe kandi zisanzwe, kandi zigatanga amakuru yimiterere ya icyitegererezo cyihariye.
Kugeza ubu, tekinoroji ikurikirana ikoreshwa cyane mu buhinzi, mu buvuzi no mu zindi nzego z’ubushakashatsi, harimo kugenzura iterambere ry’inyamaswa n’ibimera, kurwanya imihindagurikire y’ibidukikije, imikoreshereze y’imiterere y’imiterere y’imiterere y’imiterere y’imiterere y’imiterere y’imiterere y’imiterere y’imiterere y’imiterere y’imiterere y’imyororokere.
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Metagenomics (NGS)
Ihuriro ryisesengura ryateguwe kurasa imbunda ya metagenomic isesengura ryimyaka yuburambe.Igizwe nuruhererekane rwakazi rurimo ibintu bitandukanye bikenerwa cyane gusesengura metagenomics harimo gutunganya amakuru, ubushakashatsi bwurwego rwubwoko, ubushakashatsi bwurwego rwa gen, ubushakashatsi bwa metagenome, nibindi. , gushiraho ibipimo, ishusho yihariye itanga, nibindi.
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LncRNA
RNAs ndende idafite kodegisi (lncRNA) ni ubwoko bwinyandiko-mvugo ifite uburebure burenga 200 nt, idashobora kwandikisha poroteyine.Ibimenyetso bifatika byerekana ko lncRNAs nyinshi zishobora kuba zikora.Ikoreshwa ryinshi-tekinoroji ikurikirana hamwe na bioinformatic ibikoresho byo gusesengura biduha imbaraga zo guhishura urutonde rwa lncRNA no gushyira amakuru neza kandi bikatuyobora kuvumbura lncRNAs nibikorwa byingenzi byo kugenzura.BMKCloud yishimiye guha abakiriya bacu urubuga rwo gusesengura lncRNA kugirango tugere ku isesengura ryihuse, ryizewe kandi ryoroshye.
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GWAS
Kwiga ishyirahamwe rusange (GWAS) rigamije kumenya ibinyabuzima (genotype) bifitanye isano nimiterere yihariye (phenotype).Ubushakashatsi bwa GWA bugaragaza ibimenyetso byerekana ubwoko bwambukiranya genome yabantu benshi kandi bugahanura amashyirahamwe ya genotype-phenotype ukoresheje isesengura mibare kurwego rwabaturage.Ubwoko bwa genome bwuzuye bushobora kuvumbura ubwoko bwose.Ufatanije namakuru ya fenotipiki, GWAS irashobora gutunganywa kugirango hamenyekane phenotype ijyanye na SNPs, QTLs na genes zabakandida, zishyigikira cyane ubworozi bwamatungo / ibimera bigezweho.SLAF niyitezimbere ryoroheje ryogukurikirana genome ikurikirana, ivumbura genome yagutse ikwirakwizwa, SNP.Izi SNPs, nkibimenyetso bya genekulike, birashobora gutunganywa kubushakashatsi bwibikorwa bifite intego.Nuburyo buhendutse muguhitamo imico igoye ihindagurika.
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Nanopore Inyandiko-mvugo yuzuye
Isoforms igoye kandi ihindagurika mubindi binyabuzima nuburyo bwingenzi bwo kugenzura imiterere ya gene hamwe na poroteyine zitandukanye.Kumenya neza inyandiko-mvugo ni ishingiro ryokwiga byimbitse uburyo bwo kugenzura imiterere ya gene.Ikurikiranyabihe rya Nanopore ryazanye neza inyandiko-mvugo kuri isoform-urwego.Ihuriro ryisesengura ryakozwe kugirango dusesengure amakuru ya RNA-Seq yakozwe kuri platform ya Nanopore hashingiwe kuri genome yerekanwe, igera ku isesengura ryujuje ubuziranenge no mu rwego rwa gene ndetse no ku nyandiko-mvugo.
