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Ibicuruzwa

Suzuma kuri Transposase-Yagerwaho Chromatin hamwe na Byinshi Byakurikiranye (ATAC-seq)

Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq) Featured Image
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

ATAC-seq nuburyo bukurikira bwo gutondekanya uburyo bwo gusesengura genome-yagutse ya chromatine, ifite akamaro kanini mugukwirakwiza epigenetike kwisi yose.Adapteri zikurikirana zinjizwa mukarere ka chromatin zifunguye na hyperactive Tn5 transposase.Nyuma ya PCR yongerewe imbaraga, hubatswe isomero rikurikirana.Uturere twose twa chromatin dufunguye turashobora kuboneka mugihe cyumwanya-wihariye, ntabwo bigarukira gusa kumwanya uhuza ibintu byandikirwa, cyangwa akarere runaka kahinduwe.


Ibisobanuro bya serivisi

Bioinformatics

Ibisubizo bya Demo

Inyungu za serivisi

ØIsesengura Ryuzuye pip Umuyoboro wisesengura wabigize umwuga kugirango uhuze ibyifuzo bitandukanye byakorewe isesengura;

ØUbunararibonye Bwinshi projects Amajana yimishinga yarangiye neza kugeza ubu ， harimo inyamaswa n’ibiti.

Ibisobanuro bya serivisi

Ihuriro: NovaSeq 6000 platform, PE150

Ubwoko bw'isomero: 100-500 bp shyiramo isomero rya cDNA (biterwa no gukwirakwiza impinga)

Ingamba zikurikirana: Byombi-Impera 150 bp

Basabwe gusohora amakuru: 50M isoma

Icyitegererezo

Ubwoko bw'icyitegererezo: Tissue, selile nzima, cyangwa selile zahagaritswe nibindi

Inomero y'akagari: ≥ 5 × 105selile, umubare wimibare yukuri ni urufunguzo rwo gutsinda igeragezwa;

Uburemere bwinyama: ≥ 200mg tissue nshya;

Akazi ka serivisi

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Igishushanyo mbonera

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Icyitegererezo

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Gukuramo ADN

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Kubaka isomero

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Urukurikirane

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Isesengura ryamakuru

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Serivisi nyuma yo kugurisha

  • Mbere:
  • Ibikurikira:

    • Bioinformatics

    2（1）

    1. 1.Hatmap kuri ATAC isoma ikwirakwizwa kuri TSS no mukarere kegeranye (±3 kb)3（1）
    2. 2.Gusaranganya akarere ka chromatin gafunguye mubice bitandukanye bya genome4（1）3.Impinga itandukanye ihamagarira amatsinda5（1）

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