16S / 18S / ITS Ikurikirana rya Amplicon-NGS
Inyungu za serivisi
ØKwigunga no kwihuta kumenyekanisha mikorobe mubidukikije
ØIgisubizo gihanitse mubice bike-byinshi mubidukikije
ØQIIME2 iheruka gusesengura imigendekere hamwe nisesengura ritandukanye mubijyanye na base, annotation, OTU / ASV.
ØByinshi-byinjira, byukuri
ØBirakoreshwa mubyiciro bitandukanye bya mikorobe
ØBMK ifite uburambe bunini hamwe nicyitegererezo kirenga 100.000 / umwaka ， gitwikira ubutaka, amazi, gaze, isuka, umwanda, amara, uruhu, umuyonga wa fermentation, udukoko, ibimera, nibindi.
ØBMKCloud yorohereje gusobanura amakuru arimo ibikoresho 45 byo gusesengura byihariye
Ibisobanuro bya serivisi
| UrukurikiraneIhuriro | Isomero | Basabwe gutanga amakuru | Bigereranijwe guhindukira |
| Illumina NovaSeq 6000 | PE250 | 50K / 100K / 300K Tagi | Iminsi 30 |
Isesengura rya bioinformatics
üKugenzura ubuziranenge bwamakuru
üIhuriro rya OTU / De-urusaku (ASV)
üIbisobanuro bya OTU
üAlpha itandukanye
üUbwoko bwa Beta
üIsesengura hagati yitsinda
üIsesengura ryishyirahamwe rirwanya ibintu byubushakashatsi
üImikorere ya gene
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
KuriIbikuramo ADN:
| Ubwoko bw'icyitegererezo | Umubare | Kwibanda | Isuku |
| Ibikuramo ADN | ＞ 30 ng | ＞ 1 ng / μl | OD260 / 280 = 1.6-2.5 |
Kubidukikije:
| Ubwoko bw'icyitegererezo | Basabwe uburyo bwo gutoranya |
| Ubutaka | Umubare w'icyitegererezo: hafi.5 g;Ibintu bisigaye byumye bigomba gukurwa hejuru;Gusya ibice binini hanyuma unyure muri mm 2 muyungurura;Icyitegererezo cya Aliquot muri sterile EP-tube cyangwa cyrotube yo kubika. |
| Umwanda | Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot ntangarugero muri sterile EP-tube cyangwa cryotube kugirango ubike. |
| Amara | Ingero zigomba gutunganywa mugihe cya aseptic.Koza imyenda yakusanyirijwe hamwe na PBS;Centrifuge PBS hanyuma ukusanyirize imvura muri EP-tubes. |
| Umuyoboro | Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot sludge sample muri sterile EP-tube cyangwa cryotube kugirango ubike |
| Amazi | Kurugero hamwe na mikorobe nkeya, nkamazi ya robine, amazi meza, nibindi, Kusanya byibuze amazi 1 L hanyuma unyure muri 0.22 μm muyungurura kugirango ukungahaye mikorobe kuri membrane.Bika ururenda muri sterile. |
| Uruhu | Witonze witonze hejuru yuruhu ukoresheje ipamba ya sterile cyangwa urubingo rwo kubaga hanyuma ubishyire mubitereko bya sterile. |
Basabwe Gutanga Icyitegererezo
Hagarika icyitegererezo muri azote yuzuye mumasaha 3-4 hanyuma ubike muri azote yuzuye cyangwa -80 kugirango ubike igihe kirekire.Icyitegererezo cyoherezwa hamwe na ice-ice irakenewe.
Akazi ka serivisi
Icyitegererezo
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
1.Gukwirakwiza ubwoko
2.Gushushanya ikarita: Ubwoko bukize
3.Ibice bidasanzwe
4.NMDS isesengura
5.Gusesengura
Urubanza rwa BMK
Umuntu ufite umubyibuho ukabije hamwe na Diyabete yo mu bwoko bwa 2 yerekana kandi idafite ubushobozi bwo gukora mikorobe zitandukanye
Byatangajwe:Akagari ka Cell & Microbe, 2019
Ingamba zikurikirana:
Kwishingikiriza kuri diyabete (n = 633);Umubyibuho ukabije utari diyabete (n = 494);Indwara ya diyabete yo mu bwoko bwa 2 (n = 153);
Agace kagenewe: 16S rDNA V1-V2
Ihuriro: Illumina Miseq (Urutonde rwa amplicon rushingiye kuri NGS)
Igice cya ADN cyakuweho na metagenomic kuri Illumina Hiseq
Ibisubizo by'ingenzi
Microbial profilings yizi ndwara ziterwa no gutandukana neza.
Mugereranije imiterere ya mikorobe ikorwa na 16S ikurikirana, umubyibuho ukabije wasangaga ujyanye nimpinduka ziterwa na mikorobe, ibintu byihariye, cyane cyane kugabanuka gukabije kwa Akkermansia, Faecalibacterium, Oscillibacter, Alistipes, nibindi. Wongeyeho, T2D wasangaga ifitanye isano no kwiyongera kwa Escherichia / shigella .
Reba
Thingholm, LB, n'abandi.“Abantu bafite umubyibuho ukabije bafite Diyabete yo mu bwoko bwa 2 kandi badafite Ububiko butandukanye bwa mikorobe ikora neza hamwe n'imiterere.”Akagari ka Cell & Microbe26.2 (2019).
