To be a result of ours specialty and repair consciousness, our corporation has won an excellent reputation amongst customers all around the entire world for
Shotgun Sequencing Service
, Evolutionary Bioinformatics
, Shotgun Sequencing Cost
, Our main objectives are to provide our customers worldwide with good quality, competitive price, satisfied delivery and excellent services.
Reasonable price Sequencing Data Analysis -
Eukaryotic mRNA sequencing-Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
Library |
Sequencing strategy |
Data recommended |
Quality Control |
Poly A enriched |
Illumina PE150 |
6 Gb
|
Q30≥85% |
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Amount |
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation |
Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
Well-run equipment, expert income workforce, and far better after-sales expert services; We are also a unified large family, anyone stick to the corporate value "unification, dedication, tolerance" for
Reasonable price Sequencing Data Analysis -
Eukaryotic mRNA sequencing-Illumina – Biomarker , The product will supply to all over the world, such as: Barcelona
, Orlando
, Manila
, Our company regards "reasonable prices, high quality,efficient production time and good after-sales service" as our tenet. We hope to cooperate with more customers for mutual development and benefits in future. Welcome to contact us.