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Illumina Sequencing
, Qtl Mapping
, Long Read Sequencing
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Professional Design Dna Methylation Sequencing -
DNA/RNA sequencing -PacBio Sequencer – Biomarker Detail:
Features
Two sequencing modes on PacBio sequencer: CLR and CCS.
Mode |
Library |
Data yield |
Single-base accuracy |
Applications |
CLR |
20Kb, 30Kb, etc. |
80 Gb to 130 Gb/cell |
Approx. 85% |
De novo, SV calling, etc. |
CCS |
15-20 Kb |
14-40 Gb/cell (CCS) |
Approx. 99% |
De novo, Iso-Seq, SNP/Indel/SV calling |
Service Advantages
Sample Requirements
Nucleotides:
Purity |
Appearance |
Electrophoresis Gel |
Amount |
Others |
OD260/280=1.6-2.3;
OD260/230=1.5-2.5;
Clear peak at 260 nm |
Colourless, transparent liquid. |
Clear main band, limited degradation observed, no or limited protein, poly-saccharides or RNA contaminations |
Conc. ≥20 ng/μl; Volumn ≥15μl; Total amount depends on requirements in data yield. |
Concentration needs to be measured by Qubit and Qubit/Nanopore = 0.8-2.0 |
*NC/QC: NanoDrop concentration/Qubit concentration
Service Work Flow
Sample preparation
Library construction
Sequencing
Data analysis
Project delivery
Product detail pictures:
Related Product Guide:
No matter new customer or old customer, We believe in long term and trusted relationship for
Professional Design Dna Methylation Sequencing -
DNA/RNA sequencing -PacBio Sequencer – Biomarker , The product will supply to all over the world, such as: Ukraine
, Florence
, France
, We believe in establishing healthy customer relationships and positive interaction for business. Close cooperation with our customers has helped us to create strong supply chains and reap benefits. Our products have gained us widespread acceptance and the satisfaction of our worldwide valued clients.