Professional Design Dna Methylation Sequencing - DNA/RNA sequencing -PacBio Sequencer – Biomarker
Professional Design Dna Methylation Sequencing - DNA/RNA sequencing -PacBio Sequencer – Biomarker Detail:
Features
Two sequencing modes on PacBio sequencer: CLR and CCS.
| Mode | Library | Data yield | Single-base accuracy | Applications |
| CLR | 20Kb, 30Kb, etc. | 80 Gb to 130 Gb/cell | Approx. 85% | De novo, SV calling, etc. |
| CCS | 15-20 Kb | 14-40 Gb/cell (CCS) | Approx. 99% | De novo, Iso-Seq, SNP/Indel/SV calling |
Service Advantages
Sample Requirements
Nucleotides:
| Purity | Appearance | Electrophoresis Gel | Amount | Others |
| OD260/280=1.6-2.3; OD260/230=1.5-2.5； Clear peak at 260 nm |
Colourless, transparent liquid. | Clear main band, limited degradation observed, no or limited protein, poly-saccharides or RNA contaminations | Conc. ≥20 ng/μl; Volumn ≥15μl; Total amount depends on requirements in data yield. | Concentration needs to be measured by Qubit and Qubit/Nanopore = 0.8-2.0 |
*NC/QC: NanoDrop concentration/Qubit concentration
Service Work Flow
Sample preparation
Library construction
Sequencing
Data analysis
Project delivery
Product detail pictures:
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By Margaret
from Oman
- 2018.06.18 17:25
Perfect services, quality products and competitive prices, we have work many times, every time is delighted, wish continue to maintain!
By Muriel
from Greece
- 2017.03.07 13:42
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