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DNBSEQ pre-made libraries
DNBSEQ, developed by MGI, is an innovative NGS technology that has managed to decrease further down the sequencing costs and increase throughput. Preparation of DNBSEQ libraries involves DNA fragmentation, preparation of ssDNA, and rolling circle amplification to obtain the DNA nanoballs (DNB). These are then loaded onto a solid surface and subsequently sequenced by combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ technology combines the advantages of having a low amplification error rate with using high density error patterns with nanoballs, resulting in sequencing with higher throughput and accuracy.
Our pre-made library sequencing service enables customers to prepare Illumina sequencing libraries from diverse sources (mRNA, whole genome, amplicon, 10x libraries, among others), which are converted to MGI libraries in our laboratories to be sequenced in DNBSEQ-T7, enabling high data amounts at lower costs.
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Illumina pre-made libraries
Illumina sequencing technology, based on Sequencing by Synthesis (SBS), is a globally embraced NGS innovation, responsible for generating over 90% of the world’s sequencing data. The principle of SBS involves imaging fluorescently labelled reversible terminators as each dNTP is added, and subsequently cleaved to allow the incorporation of the next base. With all four reversible terminator-bound dNTPs present in each sequencing cycle, natural competition minimizes incorporation bias. This versatile technology supports both single-read and paired-end libraries, catering to a range of genomic applications. Illumina sequencing’s high-throughput capabilities and precision position it as a cornerstone in genomics research, empowering scientists to unravel the intricacies of genomes with unmatched detail and efficiency.
Our pre-made library sequencing service enables customers to prepare sequencing libraries from diverse sources (mRNA, whole genome, amplicon, 10x libraries, among others). Subsequently, these libraries can be shipped to our sequencing centers for quality control and sequencing in Illumina platforms.
