Illumina sequencing technology, based on Sequencing by Synthesis (SBS), is a globally embraced NGS innovation, responsible for generating over 90% of the world’s sequencing data. The principle of SBS involves imaging fluorescently labelled reversible terminators as each dNTP is added, and subsequently cleaved to allow the incorporation of the next base. With all four reversible terminator-bound dNTPs present in each sequencing cycle, natural competition minimizes incorporation bias. This versatile technology supports both single-read and paired-end libraries, catering to a range of genomic applications. Illumina sequencing’s high-throughput capabilities and precision position it as a cornerstone in genomics research, empowering scientists to unravel the intricacies of genomes with unmatched detail and efficiency.
Our pre-made library sequencing service enables customers to prepare sequencing libraries from diverse sources (mRNA, whole genome, amplicon, 10x libraries, among others). Subsequently, these libraries can be shipped to our sequencing centers for quality control and sequencing in Illumina platforms.