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Taking Pacific Biosciences (PacBio) Isoform sequencing data as input, this App is able to identify full-length transcript sequences (without assembly). By mapping full-length sequences against reference genome, transcripts can be optimized by known genes, transcripts, coding regions, etc. In this case, more accurate identification of mRNA structures, such as alternative splicing, etc, can be achieved. Joint analysis with NGS transcriptome sequencing data enables more comprehensive annotation and more accurate quantification in expression at transcript level, which largely benefits downstream differential expression and functional analysis.