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Online Exporter Whole Genome Sequencing Pricing -
Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
Library |
Sequencing strategy |
Data recommended |
Quality Control |
Poly A enriched |
Illumina PE150 |
6 Gb
|
Q30≥85% |
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Amount |
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation |
Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
Well-run equipment, expert income workforce, and far better after-sales expert services; We are also a unified large family, anyone stick to the corporate value "unification, dedication, tolerance" for
Online Exporter Whole Genome Sequencing Pricing -
Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Houston
, Pakistan
, Oslo
, With the technology as the core, develop and produce high-quality merchandise according to the diverse needs of the market. With this concept,the company will continue to develop merchandise with high added values and continuously improve items,and will present many customers with the best goods and services!