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One of Hottest for Pacbio Sequel Ii Cost - Nanopore-based metagenome sequencing – Biomarker

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One of Hottest for Pacbio Sequel Ii Cost - Nanopore-based metagenome sequencing – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data quality control

ü Metagenome assembly

ü Non-redundant gene set and annotation

ü Species diversity analysis

ü Genetic function diversity analysis

ü Inter-group analysis

ü Association analysis against experimental factors

Sample Requirements and Delivery

Sample requirements and delivery

Sample Requirements:

For DNA extracts:

Sample Type	Amount	Concentration	Purity
DNA extracts	＞ 30 ng	＞ 1 ng/μl	OD260/280= 1.6-2.5

For environmental samples:

Sample type	Recommended sampling procedure
Soil	Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation.
Feces	Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation.
Intestinal contents	Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes.
Sludge	Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation
Waterbody	For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube.
Skin	Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube.

Recommended Sample Delivery

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

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We know that we only thrive if we can easily guarantee our combined cost competiveness and high-quality advantageous at the same time for One of Hottest for Pacbio Sequel Ii Cost - Nanopore-based metagenome sequencing – Biomarker , The product will supply to all over the world, such as: South Africa , Detroit , Palestine , We are proud to supply our products to every costumer all around the world with our flexible, fast efficient services and strictest quality control standard which has always approved and praised by customers.

16s Rrna Sequencing Method